Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.

<h4>Background</h4>The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97ratio3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1ratio99 ratio of the Z and E isomers. Males...

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Autores principales:	Kevin W Wanner, Andrew S Nichols, Jean E Allen, Peggy L Bunger, Stephen F Garczynski, Charles E Linn, Hugh M Robertson, Charles W Luetje
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4485fdb5f4d4644a03d8951d83d7f88
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spelling	oai:doaj.org-article:c4485fdb5f4d4644a03d8951d83d7f882021-11-25T06:26:45ZSex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.1932-620310.1371/journal.pone.0008685https://doaj.org/article/c4485fdb5f4d4644a03d8951d83d7f882010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20084285/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97ratio3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1ratio99 ratio of the Z and E isomers. Males of each race respond specifically to their conspecific female's blend. A closely related species, the Asian corn borer (ACB), O. furnacalis, uses a 3ratio2 blend of Z12- and E12-tetradecenyl acetate, and is believed to have evolved from an ECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection and its evolution among closely related species we identified and characterized sex pheromone receptors from ECB(Z).<h4>Methodology</h4>Homology-dependent (degenerate PCR primers designed to conserved amino acid motifs) and homology-independent (pyrophosphate sequencing of antennal cDNA) approaches were used to identify candidate sex pheromone transcripts. Expression in male and female antennae was assayed by quantitative real-time PCR. Two-electrode voltage clamp electrophysiology was used to functionally characterize candidate receptors expressed in Xenopus oocytes.<h4>Conclusion</h4>We characterized five sex pheromone receptors, OnOrs1 and 3-6. Their transcripts were 14-100 times more abundant in male compared to female antennae. OnOr6 was highly selective for Z11-tetradecenyl acetate (EC(50) = 0.86+/-0.27 microM) and was at least three orders of magnitude less responsive to E11-tetradecenyl acetate. Surprisingly, OnOr1, 3 and 5 responded to all four pheromones tested (Z11- and E11-tetradecenyl acetate, and Z12- and E12-tetradecenyl acetate) and to Z9-tetradecenyl acetate, a behavioral antagonist. OnOr1 was selective for E12-tetradecenyl acetate based on an efficacy that was at least 5-fold greater compared to the other four components. This combination of specifically- and broadly-responsive pheromone receptors corresponds to published results of sensory neuron activity in vivo. Receptors broadly-responsive to a class of pheromone components may provide a mechanism for variation in the male moth response that enables population level shifts in pheromone blend use.Kevin W WannerAndrew S NicholsJean E AllenPeggy L BungerStephen F GarczynskiCharles E LinnHugh M RobertsonCharles W LuetjePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8685 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kevin W Wanner Andrew S Nichols Jean E Allen Peggy L Bunger Stephen F Garczynski Charles E Linn Hugh M Robertson Charles W Luetje Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
description	<h4>Background</h4>The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97ratio3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1ratio99 ratio of the Z and E isomers. Males of each race respond specifically to their conspecific female's blend. A closely related species, the Asian corn borer (ACB), O. furnacalis, uses a 3ratio2 blend of Z12- and E12-tetradecenyl acetate, and is believed to have evolved from an ECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection and its evolution among closely related species we identified and characterized sex pheromone receptors from ECB(Z).<h4>Methodology</h4>Homology-dependent (degenerate PCR primers designed to conserved amino acid motifs) and homology-independent (pyrophosphate sequencing of antennal cDNA) approaches were used to identify candidate sex pheromone transcripts. Expression in male and female antennae was assayed by quantitative real-time PCR. Two-electrode voltage clamp electrophysiology was used to functionally characterize candidate receptors expressed in Xenopus oocytes.<h4>Conclusion</h4>We characterized five sex pheromone receptors, OnOrs1 and 3-6. Their transcripts were 14-100 times more abundant in male compared to female antennae. OnOr6 was highly selective for Z11-tetradecenyl acetate (EC(50) = 0.86+/-0.27 microM) and was at least three orders of magnitude less responsive to E11-tetradecenyl acetate. Surprisingly, OnOr1, 3 and 5 responded to all four pheromones tested (Z11- and E11-tetradecenyl acetate, and Z12- and E12-tetradecenyl acetate) and to Z9-tetradecenyl acetate, a behavioral antagonist. OnOr1 was selective for E12-tetradecenyl acetate based on an efficacy that was at least 5-fold greater compared to the other four components. This combination of specifically- and broadly-responsive pheromone receptors corresponds to published results of sensory neuron activity in vivo. Receptors broadly-responsive to a class of pheromone components may provide a mechanism for variation in the male moth response that enables population level shifts in pheromone blend use.
format	article
author	Kevin W Wanner Andrew S Nichols Jean E Allen Peggy L Bunger Stephen F Garczynski Charles E Linn Hugh M Robertson Charles W Luetje
author_facet	Kevin W Wanner Andrew S Nichols Jean E Allen Peggy L Bunger Stephen F Garczynski Charles E Linn Hugh M Robertson Charles W Luetje
author_sort	Kevin W Wanner
title	Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
title_short	Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
title_full	Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
title_fullStr	Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
title_full_unstemmed	Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
title_sort	sex pheromone receptor specificity in the european corn borer moth, ostrinia nubilalis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/c4485fdb5f4d4644a03d8951d83d7f88
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Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.

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