Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity

Abstract This study evaluates the antioxidant activity of Ranunculus muricatus and isolation and structure elucidation of the active constituents. The aerial parts of the plants were shade dried at room temperature and powdered and extracted with methanol. The free radical scavenging activity was ev...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Farooq Azam, Bashir Ahmad Chaudhry, Hira Ijaz, Muhammad Imran Qadir
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
R
Q
Acceso en línea:https://doaj.org/article/c457d89c9d1a4829ad32986bc569ca48
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:c457d89c9d1a4829ad32986bc569ca48
record_format dspace
spelling oai:doaj.org-article:c457d89c9d1a4829ad32986bc569ca482021-12-02T15:09:56ZCaffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity10.1038/s41598-019-52166-w2045-2322https://doaj.org/article/c457d89c9d1a4829ad32986bc569ca482019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-52166-whttps://doaj.org/toc/2045-2322Abstract This study evaluates the antioxidant activity of Ranunculus muricatus and isolation and structure elucidation of the active constituents. The aerial parts of the plants were shade dried at room temperature and powdered and extracted with methanol. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. The percentage scavenging activity was determined based on the percentage of DPPH radical scavenged. Column chromatography was used in order to isolate the active compounds. Spectral techniques UV, IR, 1H NMR, 13CNMR and HREI-MS were used for the structure elucidation of the isolated compounds. Two isolated compounds, A (caffeoyl-β-d-glucopyranoside) and B (1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene), exibited a significant antioxidant activity as showed by DPPH radical scavenging method. Percentage inhibition for compound A (at 0.5 mM) was 82.67 ± 0.19 with IC50 of 93.25 ± 0.12 (μM), and for compound B (at 0.5 mM) was 69.23 ± 0.19 with IC50 of 183.34 ± 0.13 (μM). Quercetin was used as standard control. It was conclued from the present study that caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from methanol extract of aerial parts of Ranunculus muricatus posses antioxidant activity.Farooq AzamBashir Ahmad ChaudhryHira IjazMuhammad Imran QadirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-6 (2019)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Farooq Azam
Bashir Ahmad Chaudhry
Hira Ijaz
Muhammad Imran Qadir
Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
description Abstract This study evaluates the antioxidant activity of Ranunculus muricatus and isolation and structure elucidation of the active constituents. The aerial parts of the plants were shade dried at room temperature and powdered and extracted with methanol. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. The percentage scavenging activity was determined based on the percentage of DPPH radical scavenged. Column chromatography was used in order to isolate the active compounds. Spectral techniques UV, IR, 1H NMR, 13CNMR and HREI-MS were used for the structure elucidation of the isolated compounds. Two isolated compounds, A (caffeoyl-β-d-glucopyranoside) and B (1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene), exibited a significant antioxidant activity as showed by DPPH radical scavenging method. Percentage inhibition for compound A (at 0.5 mM) was 82.67 ± 0.19 with IC50 of 93.25 ± 0.12 (μM), and for compound B (at 0.5 mM) was 69.23 ± 0.19 with IC50 of 183.34 ± 0.13 (μM). Quercetin was used as standard control. It was conclued from the present study that caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from methanol extract of aerial parts of Ranunculus muricatus posses antioxidant activity.
format article
author Farooq Azam
Bashir Ahmad Chaudhry
Hira Ijaz
Muhammad Imran Qadir
author_facet Farooq Azam
Bashir Ahmad Chaudhry
Hira Ijaz
Muhammad Imran Qadir
author_sort Farooq Azam
title Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
title_short Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
title_full Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
title_fullStr Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
title_full_unstemmed Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity
title_sort caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(e)-nonadecene isolated from ranunculus muricatus exhibit antioxidant activity
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/c457d89c9d1a4829ad32986bc569ca48
work_keys_str_mv AT farooqazam caffeoylbdglucopyranosideand13dihydroxy2tetracosanoylamino4enonadeceneisolatedfromranunculusmuricatusexhibitantioxidantactivity
AT bashirahmadchaudhry caffeoylbdglucopyranosideand13dihydroxy2tetracosanoylamino4enonadeceneisolatedfromranunculusmuricatusexhibitantioxidantactivity
AT hiraijaz caffeoylbdglucopyranosideand13dihydroxy2tetracosanoylamino4enonadeceneisolatedfromranunculusmuricatusexhibitantioxidantactivity
AT muhammadimranqadir caffeoylbdglucopyranosideand13dihydroxy2tetracosanoylamino4enonadeceneisolatedfromranunculusmuricatusexhibitantioxidantactivity
_version_ 1718387777226145792