Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

Abstract Streptococcus uberis is one of the most frequent mastitis-causing pathogens isolated from dairy cows. Further understanding of S. uberis genetics may help elucidate the disease pathogenesis. We compared the genomes of S. uberis isolates cultured from dairy cows located in distinctly differe...

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Autores principales:	Ben Vezina, Hulayyil Al-harbi, Hena R. Ramay, Martin Soust, Robert J. Moore, Timothy W. J. Olchowy, John I. Alawneh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c45cef55b6794fe38fcb3620dcc9bb6f
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spelling	oai:doaj.org-article:c45cef55b6794fe38fcb3620dcc9bb6f2021-12-02T14:06:31ZSequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds10.1038/s41598-021-82357-32045-2322https://doaj.org/article/c45cef55b6794fe38fcb3620dcc9bb6f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82357-3https://doaj.org/toc/2045-2322Abstract Streptococcus uberis is one of the most frequent mastitis-causing pathogens isolated from dairy cows. Further understanding of S. uberis genetics may help elucidate the disease pathogenesis. We compared the genomes of S. uberis isolates cultured from dairy cows located in distinctly different geographic regions of Australia. All isolates had novel multi locus sequence types (MLST) indicating a highly diverse population of S. uberis. Global clonal complexes (GCC) were more conserved. GCC ST86 and GCC ST143 represented 30% of the total isolates (n = 27) and were clustered within different geographic regions. Core genome phylogeny revealed low phylogenetic clustering by region, isolation source, and MLST. Identification of putative sortase (srtA) substrates and generation of a custom putative virulence factor database revealed genes which may explain the affinity of S. uberis for mammary tissue, evasion of antimicrobial efforts and disease pathogenesis. Of 27 isolates, four contained antibiotic resistance genes including an antimicrobial resistance cluster containing mel/mef(A), mrsE, vatD, lnuD, and transposon-mediated lnuC was also identified. These are novel genes for S. uberis, which suggests interspecies lateral gene transfer. The presence of resistance genes across the two geographic regions tested within one country supports the need for a careful, tailored, implementation and monitoring of antimicrobial stewardship.Ben VezinaHulayyil Al-harbiHena R. RamayMartin SoustRobert J. MooreTimothy W. J. OlchowyJohn I. AlawnehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ben Vezina Hulayyil Al-harbi Hena R. Ramay Martin Soust Robert J. Moore Timothy W. J. Olchowy John I. Alawneh Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
description	Abstract Streptococcus uberis is one of the most frequent mastitis-causing pathogens isolated from dairy cows. Further understanding of S. uberis genetics may help elucidate the disease pathogenesis. We compared the genomes of S. uberis isolates cultured from dairy cows located in distinctly different geographic regions of Australia. All isolates had novel multi locus sequence types (MLST) indicating a highly diverse population of S. uberis. Global clonal complexes (GCC) were more conserved. GCC ST86 and GCC ST143 represented 30% of the total isolates (n = 27) and were clustered within different geographic regions. Core genome phylogeny revealed low phylogenetic clustering by region, isolation source, and MLST. Identification of putative sortase (srtA) substrates and generation of a custom putative virulence factor database revealed genes which may explain the affinity of S. uberis for mammary tissue, evasion of antimicrobial efforts and disease pathogenesis. Of 27 isolates, four contained antibiotic resistance genes including an antimicrobial resistance cluster containing mel/mef(A), mrsE, vatD, lnuD, and transposon-mediated lnuC was also identified. These are novel genes for S. uberis, which suggests interspecies lateral gene transfer. The presence of resistance genes across the two geographic regions tested within one country supports the need for a careful, tailored, implementation and monitoring of antimicrobial stewardship.
format	article
author	Ben Vezina Hulayyil Al-harbi Hena R. Ramay Martin Soust Robert J. Moore Timothy W. J. Olchowy John I. Alawneh
author_facet	Ben Vezina Hulayyil Al-harbi Hena R. Ramay Martin Soust Robert J. Moore Timothy W. J. Olchowy John I. Alawneh
author_sort	Ben Vezina
title	Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
title_short	Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
title_full	Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
title_fullStr	Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
title_full_unstemmed	Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds
title_sort	sequence characterisation and novel insights into bovine mastitis-associated streptococcus uberis in dairy herds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c45cef55b6794fe38fcb3620dcc9bb6f
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Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds

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