Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.

<h4>Background</h4>Epidemiological case-control studies have revealed associations between mitochondrial haplogroups and the onset and/or progression of various multifactorial diseases. For instance, mitochondrial haplogroup T was previously shown to be associated with vascular diseases,...

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Autores principales:	Edith E Mueller, Susanne M Brunner, Johannes A Mayr, Olaf Stanger, Wolfgang Sperl, Barbara Kofler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c46161ec3d96432191cd0f22f0a21578
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spelling	oai:doaj.org-article:c46161ec3d96432191cd0f22f0a215782021-11-18T08:03:43ZFunctional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.1932-620310.1371/journal.pone.0052367https://doaj.org/article/c46161ec3d96432191cd0f22f0a215782012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300652/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Epidemiological case-control studies have revealed associations between mitochondrial haplogroups and the onset and/or progression of various multifactorial diseases. For instance, mitochondrial haplogroup T was previously shown to be associated with vascular diseases, including coronary artery disease and diabetic retinopathy. In contrast, haplogroup H, the most frequent haplogroup in Europe, is often found to be more prevalent in healthy control subjects than in patient study groups. However, justifications for the assumption that haplogroups are functionally distinct are rare. Therefore, we attempted to compare differences in mitochondrial function between haplogroup H and T cybrids.<h4>Methodology/principal findings</h4>Mitochondrial haplogroup H and T cybrids were generated by fusion of HEK293 cells devoid of mitochondrial DNA with isolated thrombocytes of individuals with the respective haplogroups. These cybrid cells were analyzed for oxidative phosphorylation (OXPHOS) enzyme activities, mitochondrial DNA (mtDNA) copy number, growth rate and susceptibility to reactive oxygen species (ROS). We observed that haplogroup T cybrids have higher survival rate when challenged with hydrogen peroxide, indicating a higher capability to cope with oxidative stress.<h4>Conclusions/significance</h4>The results of this study show that functional differences exist between HEK293 cybrid cells which differ in mitochondrial genomic background.Edith E MuellerSusanne M BrunnerJohannes A MayrOlaf StangerWolfgang SperlBarbara KoflerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52367 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edith E Mueller Susanne M Brunner Johannes A Mayr Olaf Stanger Wolfgang Sperl Barbara Kofler Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
description	<h4>Background</h4>Epidemiological case-control studies have revealed associations between mitochondrial haplogroups and the onset and/or progression of various multifactorial diseases. For instance, mitochondrial haplogroup T was previously shown to be associated with vascular diseases, including coronary artery disease and diabetic retinopathy. In contrast, haplogroup H, the most frequent haplogroup in Europe, is often found to be more prevalent in healthy control subjects than in patient study groups. However, justifications for the assumption that haplogroups are functionally distinct are rare. Therefore, we attempted to compare differences in mitochondrial function between haplogroup H and T cybrids.<h4>Methodology/principal findings</h4>Mitochondrial haplogroup H and T cybrids were generated by fusion of HEK293 cells devoid of mitochondrial DNA with isolated thrombocytes of individuals with the respective haplogroups. These cybrid cells were analyzed for oxidative phosphorylation (OXPHOS) enzyme activities, mitochondrial DNA (mtDNA) copy number, growth rate and susceptibility to reactive oxygen species (ROS). We observed that haplogroup T cybrids have higher survival rate when challenged with hydrogen peroxide, indicating a higher capability to cope with oxidative stress.<h4>Conclusions/significance</h4>The results of this study show that functional differences exist between HEK293 cybrid cells which differ in mitochondrial genomic background.
format	article
author	Edith E Mueller Susanne M Brunner Johannes A Mayr Olaf Stanger Wolfgang Sperl Barbara Kofler
author_facet	Edith E Mueller Susanne M Brunner Johannes A Mayr Olaf Stanger Wolfgang Sperl Barbara Kofler
author_sort	Edith E Mueller
title	Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
title_short	Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
title_full	Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
title_fullStr	Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
title_full_unstemmed	Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.
title_sort	functional differences between mitochondrial haplogroup t and haplogroup h in hek293 cybrid cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c46161ec3d96432191cd0f22f0a21578
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Functional differences between mitochondrial haplogroup T and haplogroup H in HEK293 cybrid cells.

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