Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery

Abstract Magnetic nanoparticles (MNPs, Fe3O4) incorporated into the complexes of cell penetrating peptides (CPPs)-oligonucleotides (ONs) promoted the cell transfection for plasmid transfection, splice correction, and gene silencing efficiencies. Six types of cell penetrating peptides (CPPs; PeptFect...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Moataz Dowaidar, Hani Nasser Abdelhamid, Mattias Hällbrink, Krista Freimann, Kaido Kurrikoff, Xiaodong Zou, Ülo Langel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4631fac3dd145529679391dcb43aa18
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c4631fac3dd145529679391dcb43aa18
record_format	dspace
spelling	oai:doaj.org-article:c4631fac3dd145529679391dcb43aa182021-12-02T16:06:40ZMagnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery10.1038/s41598-017-09803-z2045-2322https://doaj.org/article/c4631fac3dd145529679391dcb43aa182017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09803-zhttps://doaj.org/toc/2045-2322Abstract Magnetic nanoparticles (MNPs, Fe3O4) incorporated into the complexes of cell penetrating peptides (CPPs)-oligonucleotides (ONs) promoted the cell transfection for plasmid transfection, splice correction, and gene silencing efficiencies. Six types of cell penetrating peptides (CPPs; PeptFect220 (denoted PF220), PF221, PF222, PF223, PF224 and PF14) and three types of gene therapeutic agents (plasmid (pGL3), splicing correcting oligonucleotides (SCO), and small interfering RNA (siRNA) were investigated. Magnetic nanoparticles incorporated into the complexes of CPPs-pGL3, CPPs-SCO, and CPPs-siRNA showed high cell biocompatibility and efficiently transfected the investigated cells with pGL3, SCO, and siRNA, respectively. Gene transfer vectors formed among PF14, SCO, and MNPs (PF14-SCO-MNPs) showed a superior transfection efficiency (up to 4-fold) compared to the noncovalent PF14-SCO complex, which was previously reported with a higher efficiency compared to commercial vector called Lipofectamine™2000. The high transfection efficiency of the new complexes (CPPs-SCO-MNPs) may be attributed to the morphology, low cytotoxicity, and the synergistic effect of MNPs and CPPs. PF14-pDNA-MNPs is an efficient complex for in vivo gene delivery upon systemic administration. The conjugation of CPPs-ONs with inorganic magnetic nanoparticles (Fe3O4) may open new venues for selective and efficient gene therapy.Moataz DowaidarHani Nasser AbdelhamidMattias HällbrinkKrista FreimannKaido KurrikoffXiaodong ZouÜlo LangelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moataz Dowaidar Hani Nasser Abdelhamid Mattias Hällbrink Krista Freimann Kaido Kurrikoff Xiaodong Zou Ülo Langel Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
description	Abstract Magnetic nanoparticles (MNPs, Fe3O4) incorporated into the complexes of cell penetrating peptides (CPPs)-oligonucleotides (ONs) promoted the cell transfection for plasmid transfection, splice correction, and gene silencing efficiencies. Six types of cell penetrating peptides (CPPs; PeptFect220 (denoted PF220), PF221, PF222, PF223, PF224 and PF14) and three types of gene therapeutic agents (plasmid (pGL3), splicing correcting oligonucleotides (SCO), and small interfering RNA (siRNA) were investigated. Magnetic nanoparticles incorporated into the complexes of CPPs-pGL3, CPPs-SCO, and CPPs-siRNA showed high cell biocompatibility and efficiently transfected the investigated cells with pGL3, SCO, and siRNA, respectively. Gene transfer vectors formed among PF14, SCO, and MNPs (PF14-SCO-MNPs) showed a superior transfection efficiency (up to 4-fold) compared to the noncovalent PF14-SCO complex, which was previously reported with a higher efficiency compared to commercial vector called Lipofectamine™2000. The high transfection efficiency of the new complexes (CPPs-SCO-MNPs) may be attributed to the morphology, low cytotoxicity, and the synergistic effect of MNPs and CPPs. PF14-pDNA-MNPs is an efficient complex for in vivo gene delivery upon systemic administration. The conjugation of CPPs-ONs with inorganic magnetic nanoparticles (Fe3O4) may open new venues for selective and efficient gene therapy.
format	article
author	Moataz Dowaidar Hani Nasser Abdelhamid Mattias Hällbrink Krista Freimann Kaido Kurrikoff Xiaodong Zou Ülo Langel
author_facet	Moataz Dowaidar Hani Nasser Abdelhamid Mattias Hällbrink Krista Freimann Kaido Kurrikoff Xiaodong Zou Ülo Langel
author_sort	Moataz Dowaidar
title	Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
title_short	Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
title_full	Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
title_fullStr	Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
title_full_unstemmed	Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery
title_sort	magnetic nanoparticle assisted self-assembly of cell penetrating peptides-oligonucleotides complexes for gene delivery
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c4631fac3dd145529679391dcb43aa18
work_keys_str_mv	AT moatazdowaidar magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT haninasserabdelhamid magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT mattiashallbrink magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT kristafreimann magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT kaidokurrikoff magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT xiaodongzou magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery AT ulolangel magneticnanoparticleassistedselfassemblyofcellpenetratingpeptidesoligonucleotidescomplexesforgenedelivery
_version_	1718384929405927424

Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery

Ejemplares similares