Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

Abstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension...

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Autores principales:	Łukasz Richter, Karolina Księżarczyk, Karolina Paszkowska, Marta Janczuk-Richter, Joanna Niedziółka-Jönsson, Jacek Gapiński, Marcin Łoś, Robert Hołyst, Jan Paczesny
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c466dbe6c3284005b81ea654db2a385c
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spelling	oai:doaj.org-article:c466dbe6c3284005b81ea654db2a385c2021-12-02T14:23:32ZAdsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research10.1038/s41598-021-86571-x2045-2322https://doaj.org/article/c466dbe6c3284005b81ea654db2a385c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86571-xhttps://doaj.org/toc/2045-2322Abstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.Łukasz RichterKarolina KsiężarczykKarolina PaszkowskaMarta Janczuk-RichterJoanna Niedziółka-JönssonJacek GapińskiMarcin ŁośRobert HołystJan PaczesnyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
description	Abstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.
format	article
author	Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny
author_facet	Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny
author_sort	Łukasz Richter
title	Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_short	Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_full	Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_fullStr	Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_full_unstemmed	Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
title_sort	adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c466dbe6c3284005b81ea654db2a385c
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Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

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