Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease

CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.

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Autores principales:	Fernando Orden Rueda, Michal Bista, Matthew D. Newton, Anne U. Goeppert, M. Emanuela Cuomo, Euan Gordon, Felix Kröner, Jon A. Read, Jonathan D. Wrigley, David Rueda, Benjamin J. M. Taylor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c46a36e939634bf2b4da5347a251be8a
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spelling	oai:doaj.org-article:c46a36e939634bf2b4da5347a251be8a2021-12-02T16:51:52ZMapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease10.1038/s41467-017-01732-92041-1723https://doaj.org/article/c46a36e939634bf2b4da5347a251be8a2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01732-9https://doaj.org/toc/2041-1723CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.Fernando Orden RuedaMichal BistaMatthew D. NewtonAnne U. GoeppertM. Emanuela CuomoEuan GordonFelix KrönerJon A. ReadJonathan D. WrigleyDavid RuedaBenjamin J. M. TaylorNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fernando Orden Rueda Michal Bista Matthew D. Newton Anne U. Goeppert M. Emanuela Cuomo Euan Gordon Felix Kröner Jon A. Read Jonathan D. Wrigley David Rueda Benjamin J. M. Taylor Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
description	CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.
format	article
author	Fernando Orden Rueda Michal Bista Matthew D. Newton Anne U. Goeppert M. Emanuela Cuomo Euan Gordon Felix Kröner Jon A. Read Jonathan D. Wrigley David Rueda Benjamin J. M. Taylor
author_facet	Fernando Orden Rueda Michal Bista Matthew D. Newton Anne U. Goeppert M. Emanuela Cuomo Euan Gordon Felix Kröner Jon A. Read Jonathan D. Wrigley David Rueda Benjamin J. M. Taylor
author_sort	Fernando Orden Rueda
title	Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
title_short	Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
title_full	Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
title_fullStr	Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
title_full_unstemmed	Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
title_sort	mapping the sugar dependency for rational generation of a dna-rna hybrid-guided cas9 endonuclease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c46a36e939634bf2b4da5347a251be8a
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Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease

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