Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.
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Main Authors: | , , , , , , , , , , |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2017
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Subjects: | |
Online Access: | https://doaj.org/article/c46a36e939634bf2b4da5347a251be8a |
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