Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3)
Ribosomal protein S3 (rpS3), a member of 40S small ribosomal subunit, is a multifunctional protein with various extra-ribosomal functions including DNA repair endonuclease activity and is secreted from cancer cells. Therefore, antibodies with high specificity against rpS3 protein could be useful can...
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Taylor & Francis Group
2021
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oai:doaj.org-article:c46a4749573c4aa58c8e0328226a25992021-11-04T15:00:42ZProduction, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3)1976-83542151-248510.1080/19768354.2021.1980100https://doaj.org/article/c46a4749573c4aa58c8e0328226a25992021-09-01T00:00:00Zhttp://dx.doi.org/10.1080/19768354.2021.1980100https://doaj.org/toc/1976-8354https://doaj.org/toc/2151-2485Ribosomal protein S3 (rpS3), a member of 40S small ribosomal subunit, is a multifunctional protein with various extra-ribosomal functions including DNA repair endonuclease activity and is secreted from cancer cells. Therefore, antibodies with high specificity against rpS3 protein could be useful cancer biomarkers. In this study, polyclonal antibody (pAb) and monoclonal antibodies (mAbs) were raised against rpS3 protein and epitope mapping was performed for each antibody; the amino acid residues of rpS3 were scanned from amino acid 185 to 243 through peptide scanning to reveal the epitopes of each mAb. Results showed that pAb R2 has an epitope from amino acid 203 to 230, mAb M7 has an epitope from amino acid 213 to 221, and mAb M8 has an epitope from amino acid 197 to 219. Taken together, novel mAbs and pAb against rpS3 were raised and mapped against rpS3 with different specific epitopes.Woo-Sung AhnTae-Sung KimYong Jun ParkYoung Kwang ParkHag Dong KimJoon KimTaylor & Francis Grouparticleribosomal protein subunit small 3epitope mappingmabpeptide synthesisdirect elisasandwich elisalinear form epitopeconformational epitopeimmunoprecipitationMedicine (General)R5-920Biology (General)QH301-705.5ENAnimal Cells and Systems, Vol 25, Iss 5, Pp 323-336 (2021) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
ribosomal protein subunit small 3 epitope mapping mab peptide synthesis direct elisa sandwich elisa linear form epitope conformational epitope immunoprecipitation Medicine (General) R5-920 Biology (General) QH301-705.5 |
| spellingShingle |
ribosomal protein subunit small 3 epitope mapping mab peptide synthesis direct elisa sandwich elisa linear form epitope conformational epitope immunoprecipitation Medicine (General) R5-920 Biology (General) QH301-705.5 Woo-Sung Ahn Tae-Sung Kim Yong Jun Park Young Kwang Park Hag Dong Kim Joon Kim Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| description |
Ribosomal protein S3 (rpS3), a member of 40S small ribosomal subunit, is a multifunctional protein with various extra-ribosomal functions including DNA repair endonuclease activity and is secreted from cancer cells. Therefore, antibodies with high specificity against rpS3 protein could be useful cancer biomarkers. In this study, polyclonal antibody (pAb) and monoclonal antibodies (mAbs) were raised against rpS3 protein and epitope mapping was performed for each antibody; the amino acid residues of rpS3 were scanned from amino acid 185 to 243 through peptide scanning to reveal the epitopes of each mAb. Results showed that pAb R2 has an epitope from amino acid 203 to 230, mAb M7 has an epitope from amino acid 213 to 221, and mAb M8 has an epitope from amino acid 197 to 219. Taken together, novel mAbs and pAb against rpS3 were raised and mapped against rpS3 with different specific epitopes. |
| format |
article |
| author |
Woo-Sung Ahn Tae-Sung Kim Yong Jun Park Young Kwang Park Hag Dong Kim Joon Kim |
| author_facet |
Woo-Sung Ahn Tae-Sung Kim Yong Jun Park Young Kwang Park Hag Dong Kim Joon Kim |
| author_sort |
Woo-Sung Ahn |
| title |
Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| title_short |
Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| title_full |
Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| title_fullStr |
Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| title_full_unstemmed |
Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3) |
| title_sort |
production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein s3 (rps3) |
| publisher |
Taylor & Francis Group |
| publishDate |
2021 |
| url |
https://doaj.org/article/c46a4749573c4aa58c8e0328226a2599 |
| work_keys_str_mv |
AT woosungahn productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 AT taesungkim productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 AT yongjunpark productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 AT youngkwangpark productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 AT hagdongkim productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 AT joonkim productioncharacterizationandepitopemappingofmonoclonalantibodiesofribosomalproteins3rps3 |
| _version_ |
1718444796833431552 |