Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.

Chloride intracellular Channel 1 (CLIC1) is a metamorphic protein that changes from a soluble cytoplasmic protein into a transmembrane protein. Once inserted into membranes, CLIC1 multimerises and is able to form chloride selective ion channels. Whilst CLIC1 behaves as an ion channel both in cells a...

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Autores principales:	Stefania Averaimo, Rosella Abeti, Nicoletta Savalli, Louise J Brown, Paul M G Curmi, Samuel N Breit, Michele Mazzanti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c46ef3aaca374bb39244bdde8d74f8bd
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spelling	oai:doaj.org-article:c46ef3aaca374bb39244bdde8d74f8bd2021-11-18T08:54:44ZPoint mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.1932-620310.1371/journal.pone.0074523https://doaj.org/article/c46ef3aaca374bb39244bdde8d74f8bd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24058583/?tool=EBIhttps://doaj.org/toc/1932-6203Chloride intracellular Channel 1 (CLIC1) is a metamorphic protein that changes from a soluble cytoplasmic protein into a transmembrane protein. Once inserted into membranes, CLIC1 multimerises and is able to form chloride selective ion channels. Whilst CLIC1 behaves as an ion channel both in cells and in artificial lipid bilayers, its structure in the soluble form has led to some uncertainty as to whether it really is an ion channel protein. CLIC1 has a single putative transmembrane region that contains only two charged residues: arginine 29 (Arg29) and lysine 37 (Lys37). As charged residues are likely to have a key role in ion channel function, we hypothesized that mutating them to neutral alanine to generate K37A and R29A CLIC1 would alter the electrophysiological characteristics of CLIC1. By using three different electrophysiological approaches: i) single channel Tip-Dip in artificial bilayers using soluble recombinant CLIC1, ii) cell-attached and iii) whole-cell patch clamp recordings in transiently transfected HEK cells, we determined that the K37A mutation altered the single-channel conductance while the R29A mutation affected the single-channel open probability in response to variation in membrane potential. Our results show that mutation of the two charged amino acids (K37 and R29) in the putative transmembrane region of CLIC1 alters the biophysical properties of the ion channel in both artificial bilayers and cells. Hence these charged residues are directly involved in regulating its ion channel activity. This strongly suggests that, despite its unusual structure, CLIC1 itself is able to form a chloride ion channel.Stefania AveraimoRosella AbetiNicoletta SavalliLouise J BrownPaul M G CurmiSamuel N BreitMichele MazzantiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74523 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefania Averaimo Rosella Abeti Nicoletta Savalli Louise J Brown Paul M G Curmi Samuel N Breit Michele Mazzanti Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
description	Chloride intracellular Channel 1 (CLIC1) is a metamorphic protein that changes from a soluble cytoplasmic protein into a transmembrane protein. Once inserted into membranes, CLIC1 multimerises and is able to form chloride selective ion channels. Whilst CLIC1 behaves as an ion channel both in cells and in artificial lipid bilayers, its structure in the soluble form has led to some uncertainty as to whether it really is an ion channel protein. CLIC1 has a single putative transmembrane region that contains only two charged residues: arginine 29 (Arg29) and lysine 37 (Lys37). As charged residues are likely to have a key role in ion channel function, we hypothesized that mutating them to neutral alanine to generate K37A and R29A CLIC1 would alter the electrophysiological characteristics of CLIC1. By using three different electrophysiological approaches: i) single channel Tip-Dip in artificial bilayers using soluble recombinant CLIC1, ii) cell-attached and iii) whole-cell patch clamp recordings in transiently transfected HEK cells, we determined that the K37A mutation altered the single-channel conductance while the R29A mutation affected the single-channel open probability in response to variation in membrane potential. Our results show that mutation of the two charged amino acids (K37 and R29) in the putative transmembrane region of CLIC1 alters the biophysical properties of the ion channel in both artificial bilayers and cells. Hence these charged residues are directly involved in regulating its ion channel activity. This strongly suggests that, despite its unusual structure, CLIC1 itself is able to form a chloride ion channel.
format	article
author	Stefania Averaimo Rosella Abeti Nicoletta Savalli Louise J Brown Paul M G Curmi Samuel N Breit Michele Mazzanti
author_facet	Stefania Averaimo Rosella Abeti Nicoletta Savalli Louise J Brown Paul M G Curmi Samuel N Breit Michele Mazzanti
author_sort	Stefania Averaimo
title	Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
title_short	Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
title_full	Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
title_fullStr	Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
title_full_unstemmed	Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
title_sort	point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c46ef3aaca374bb39244bdde8d74f8bd
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Point mutations in the transmembrane region of the clic1 ion channel selectively modify its biophysical properties.

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