Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results

Alahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histologi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alsmman AH, Radwan G, Abozaid MA, Mohammed UA, Abd Elhaliem NG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	subconjunctival bevacizumab subconjunctival mitomycin C histolog-ical changes primary pterygium CD31. Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/c47a06f8c101479ca218918fa60a1633
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c47a06f8c101479ca218918fa60a1633
record_format	dspace
spelling	oai:doaj.org-article:c47a06f8c101479ca218918fa60a16332021-12-02T00:25:39ZPreoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results1177-5483https://doaj.org/article/c47a06f8c101479ca218918fa60a16332017-03-01T00:00:00Zhttps://www.dovepress.com/preoperative-subconjunctival-combined-injection-of-bevacizumab-and-mit-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Alahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histological effects of preoperative subconjunctival injection of both bevacizumab and mitomycin C (MMC) 1 month before the surgical excision of primary pterygium using a bare sclera technique. Patients and methods: A total of 20 patients with primary pterygium underwent subconjunctival combined injection of 0.1 mL of MMC (0.1 mg/mL) and 0.1 mL of bevacizumab (1.25 mg/0.1 mL) 1 month before bare sclera excision of the pterygium. The excised pterygium tissues were examined histologically and immunohistologically by CD31 staining, and the patients were followed up clinically for at least 2 years. The excised pterygia of two patients without preoperative injection were used for histological comparison. Results: Clinically, there were no intraoperative or postoperative complications. No recurrence was noted during the follow-up period. Histologically, the previously injected pterygia showed a decreased number of epithelial cells and stromal fibroblasts. The latter were rounded or oval and swollen rather than spindle shaped, and some were degenerating or apoptotic. Collagen and elastic fibers were degenerated, distorted, and decreased in density, while blood capillaries were obliterated. There was a significant decrease in CD31-positive cells in previously injected pterygia. Conclusion: Preoperative subpterygium combined injection of bevacizumab and MMC is safe and effective in reducing the postoperative recurrence of primary pterygium. Histological and immunohistological changes in the form of decreased fibrovascular activity and degeneration of the extracellular matrix and nerve axons were noted. Keywords: subconjunctival bevacizumab, subconjunctival mitomycin C, histological changes, primary pterygium, CD31Alsmman AHRadwan GAbozaid MAMohammed UAAbd Elhaliem NGDove Medical Pressarticlesubconjunctival bevacizumabsubconjunctival mitomycin Chistolog-ical changesprimary pterygiumCD31.OphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 493-501 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	subconjunctival bevacizumab subconjunctival mitomycin C histolog-ical changes primary pterygium CD31. Ophthalmology RE1-994
spellingShingle	subconjunctival bevacizumab subconjunctival mitomycin C histolog-ical changes primary pterygium CD31. Ophthalmology RE1-994 Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
description	Alahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histological effects of preoperative subconjunctival injection of both bevacizumab and mitomycin C (MMC) 1 month before the surgical excision of primary pterygium using a bare sclera technique. Patients and methods: A total of 20 patients with primary pterygium underwent subconjunctival combined injection of 0.1 mL of MMC (0.1 mg/mL) and 0.1 mL of bevacizumab (1.25 mg/0.1 mL) 1 month before bare sclera excision of the pterygium. The excised pterygium tissues were examined histologically and immunohistologically by CD31 staining, and the patients were followed up clinically for at least 2 years. The excised pterygia of two patients without preoperative injection were used for histological comparison. Results: Clinically, there were no intraoperative or postoperative complications. No recurrence was noted during the follow-up period. Histologically, the previously injected pterygia showed a decreased number of epithelial cells and stromal fibroblasts. The latter were rounded or oval and swollen rather than spindle shaped, and some were degenerating or apoptotic. Collagen and elastic fibers were degenerated, distorted, and decreased in density, while blood capillaries were obliterated. There was a significant decrease in CD31-positive cells in previously injected pterygia. Conclusion: Preoperative subpterygium combined injection of bevacizumab and MMC is safe and effective in reducing the postoperative recurrence of primary pterygium. Histological and immunohistological changes in the form of decreased fibrovascular activity and degeneration of the extracellular matrix and nerve axons were noted. Keywords: subconjunctival bevacizumab, subconjunctival mitomycin C, histological changes, primary pterygium, CD31
format	article
author	Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG
author_facet	Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG
author_sort	Alsmman AH
title	Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
title_short	Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
title_full	Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
title_fullStr	Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
title_full_unstemmed	Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
title_sort	preoperative subconjunctival combined injection of bevacizumab and mitomycin c before the surgical excision of primary pterygium: clinical and histological results
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/c47a06f8c101479ca218918fa60a1633
work_keys_str_mv	AT alsmmanah preoperativesubconjunctivalcombinedinjectionofbevacizumabandmitomycincbeforethesurgicalexcisionofprimarypterygiumclinicalandhistologicalresults AT radwang preoperativesubconjunctivalcombinedinjectionofbevacizumabandmitomycincbeforethesurgicalexcisionofprimarypterygiumclinicalandhistologicalresults AT abozaidma preoperativesubconjunctivalcombinedinjectionofbevacizumabandmitomycincbeforethesurgicalexcisionofprimarypterygiumclinicalandhistologicalresults AT mohammedua preoperativesubconjunctivalcombinedinjectionofbevacizumabandmitomycincbeforethesurgicalexcisionofprimarypterygiumclinicalandhistologicalresults AT abdelhaliemng preoperativesubconjunctivalcombinedinjectionofbevacizumabandmitomycincbeforethesurgicalexcisionofprimarypterygiumclinicalandhistologicalresults
_version_	1718403765418065920

Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results

Ejemplares similares