Ethanol affects behavior and HPA axis activity during development in zebrafish larvae

Abstract Recent studies have shown that long-term alcohol intake from food can lead to numerous mental disorders in humans, and the social and health effects of excessive intake of alcohol currently represent serious problems for governments and families worldwide. However, to date, it has not been...

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Autores principales:	Wenxiao Du, Xiaoli Chen, Min Shi, Fuhua Bian, Zhenjun Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c480ddac92b34dba82da6c538c9c690a
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spelling	oai:doaj.org-article:c480ddac92b34dba82da6c538c9c690a2021-12-02T11:43:44ZEthanol affects behavior and HPA axis activity during development in zebrafish larvae10.1038/s41598-020-78573-y2045-2322https://doaj.org/article/c480ddac92b34dba82da6c538c9c690a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78573-yhttps://doaj.org/toc/2045-2322Abstract Recent studies have shown that long-term alcohol intake from food can lead to numerous mental disorders in humans, and the social and health effects of excessive intake of alcohol currently represent serious problems for governments and families worldwide. However, to date, it has not been determined how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. The zebrafish offers a good model for studying the toxicology of food-grade ethanol. In the present study, using zebrafish larvae exposed to 1% ethanol, we performed zebrafish behavioral analysis. Samples were collected for enzyme-linked immunosorbent assay (ELISA) and quantitative real time-polymerase chain reaction (qRT-PCR) experiments, and statistical analysis was performed. We found that ethanol decreased the locomotor activity of zebrafish larvae, which showed a more intense reaction to external stimuli. Ethanol also increased the level of HPA axis hormones in zebrafish larvae, influenced the level of neurotransmitters, and altered the expression of key genes in neurotransmitter metabolism. Ethanol exposure affects zebrafish behavior, increases the level of HPA axis hormones in zebrafish larvae, affects the level of neurotransmitters, and affects the expression of key genes in dopamine and serotonin metabolism. These findings may help to elucidate the effects of ethanol on HPA axis activity.Wenxiao DuXiaoli ChenMin ShiFuhua BianZhenjun ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenxiao Du Xiaoli Chen Min Shi Fuhua Bian Zhenjun Zhao Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
description	Abstract Recent studies have shown that long-term alcohol intake from food can lead to numerous mental disorders in humans, and the social and health effects of excessive intake of alcohol currently represent serious problems for governments and families worldwide. However, to date, it has not been determined how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. The zebrafish offers a good model for studying the toxicology of food-grade ethanol. In the present study, using zebrafish larvae exposed to 1% ethanol, we performed zebrafish behavioral analysis. Samples were collected for enzyme-linked immunosorbent assay (ELISA) and quantitative real time-polymerase chain reaction (qRT-PCR) experiments, and statistical analysis was performed. We found that ethanol decreased the locomotor activity of zebrafish larvae, which showed a more intense reaction to external stimuli. Ethanol also increased the level of HPA axis hormones in zebrafish larvae, influenced the level of neurotransmitters, and altered the expression of key genes in neurotransmitter metabolism. Ethanol exposure affects zebrafish behavior, increases the level of HPA axis hormones in zebrafish larvae, affects the level of neurotransmitters, and affects the expression of key genes in dopamine and serotonin metabolism. These findings may help to elucidate the effects of ethanol on HPA axis activity.
format	article
author	Wenxiao Du Xiaoli Chen Min Shi Fuhua Bian Zhenjun Zhao
author_facet	Wenxiao Du Xiaoli Chen Min Shi Fuhua Bian Zhenjun Zhao
author_sort	Wenxiao Du
title	Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
title_short	Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
title_full	Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
title_fullStr	Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
title_full_unstemmed	Ethanol affects behavior and HPA axis activity during development in zebrafish larvae
title_sort	ethanol affects behavior and hpa axis activity during development in zebrafish larvae
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c480ddac92b34dba82da6c538c9c690a
work_keys_str_mv	AT wenxiaodu ethanolaffectsbehaviorandhpaaxisactivityduringdevelopmentinzebrafishlarvae AT xiaolichen ethanolaffectsbehaviorandhpaaxisactivityduringdevelopmentinzebrafishlarvae AT minshi ethanolaffectsbehaviorandhpaaxisactivityduringdevelopmentinzebrafishlarvae AT fuhuabian ethanolaffectsbehaviorandhpaaxisactivityduringdevelopmentinzebrafishlarvae AT zhenjunzhao ethanolaffectsbehaviorandhpaaxisactivityduringdevelopmentinzebrafishlarvae
_version_	1718395368145682432

Ethanol affects behavior and HPA axis activity during development in zebrafish larvae

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