A microtubule interactome: complexes with roles in cell cycle and mitosis.

The microtubule (MT) cytoskeleton is required for many aspects of cell function, including the transport of intracellular materials, the maintenance of cell polarity, and the regulation of mitosis. These functions are coordinated by MT-associated proteins (MAPs), which work in concert with each othe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julian R Hughes, Ana M Meireles, Katherine H Fisher, Angel Garcia, Philip R Antrobus, Alan Wainman, Nicole Zitzmann, Charlotte Deane, Hiroyuki Ohkura, James G Wakefield
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c4838029fa1c4a1d88aa8e3272f35393
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c4838029fa1c4a1d88aa8e3272f35393
record_format	dspace
spelling	oai:doaj.org-article:c4838029fa1c4a1d88aa8e3272f353932021-12-02T19:54:31ZA microtubule interactome: complexes with roles in cell cycle and mitosis.1544-91731545-788510.1371/journal.pbio.0060098https://doaj.org/article/c4838029fa1c4a1d88aa8e3272f353932008-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18433294/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The microtubule (MT) cytoskeleton is required for many aspects of cell function, including the transport of intracellular materials, the maintenance of cell polarity, and the regulation of mitosis. These functions are coordinated by MT-associated proteins (MAPs), which work in concert with each other, binding MTs and altering their properties. We have used a MT cosedimentation assay, combined with 1D and 2D PAGE and mass spectrometry, to identify over 250 MAPs from early Drosophila embryos. We have taken two complementary approaches to analyse the cellular function of novel MAPs isolated using this approach. First, we have carried out an RNA interference (RNAi) screen, identifying 21 previously uncharacterised genes involved in MT organisation. Second, we have undertaken a bioinformatics analysis based on binary protein interaction data to produce putative interaction networks of MAPs. By combining both approaches, we have identified and validated MAP complexes with potentially important roles in cell cycle regulation and mitosis. This study therefore demonstrates that biologically relevant data can be harvested using such a multidisciplinary approach, and identifies new MAPs, many of which appear to be important in cell division.Julian R HughesAna M MeirelesKatherine H FisherAngel GarciaPhilip R AntrobusAlan WainmanNicole ZitzmannCharlotte DeaneHiroyuki OhkuraJames G WakefieldPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 4, p e98 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Julian R Hughes Ana M Meireles Katherine H Fisher Angel Garcia Philip R Antrobus Alan Wainman Nicole Zitzmann Charlotte Deane Hiroyuki Ohkura James G Wakefield A microtubule interactome: complexes with roles in cell cycle and mitosis.
description	The microtubule (MT) cytoskeleton is required for many aspects of cell function, including the transport of intracellular materials, the maintenance of cell polarity, and the regulation of mitosis. These functions are coordinated by MT-associated proteins (MAPs), which work in concert with each other, binding MTs and altering their properties. We have used a MT cosedimentation assay, combined with 1D and 2D PAGE and mass spectrometry, to identify over 250 MAPs from early Drosophila embryos. We have taken two complementary approaches to analyse the cellular function of novel MAPs isolated using this approach. First, we have carried out an RNA interference (RNAi) screen, identifying 21 previously uncharacterised genes involved in MT organisation. Second, we have undertaken a bioinformatics analysis based on binary protein interaction data to produce putative interaction networks of MAPs. By combining both approaches, we have identified and validated MAP complexes with potentially important roles in cell cycle regulation and mitosis. This study therefore demonstrates that biologically relevant data can be harvested using such a multidisciplinary approach, and identifies new MAPs, many of which appear to be important in cell division.
format	article
author	Julian R Hughes Ana M Meireles Katherine H Fisher Angel Garcia Philip R Antrobus Alan Wainman Nicole Zitzmann Charlotte Deane Hiroyuki Ohkura James G Wakefield
author_facet	Julian R Hughes Ana M Meireles Katherine H Fisher Angel Garcia Philip R Antrobus Alan Wainman Nicole Zitzmann Charlotte Deane Hiroyuki Ohkura James G Wakefield
author_sort	Julian R Hughes
title	A microtubule interactome: complexes with roles in cell cycle and mitosis.
title_short	A microtubule interactome: complexes with roles in cell cycle and mitosis.
title_full	A microtubule interactome: complexes with roles in cell cycle and mitosis.
title_fullStr	A microtubule interactome: complexes with roles in cell cycle and mitosis.
title_full_unstemmed	A microtubule interactome: complexes with roles in cell cycle and mitosis.
title_sort	microtubule interactome: complexes with roles in cell cycle and mitosis.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/c4838029fa1c4a1d88aa8e3272f35393
work_keys_str_mv	AT julianrhughes amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT anammeireles amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT katherinehfisher amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT angelgarcia amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT philiprantrobus amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT alanwainman amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT nicolezitzmann amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT charlottedeane amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT hiroyukiohkura amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT jamesgwakefield amicrotubuleinteractomecomplexeswithrolesincellcycleandmitosis AT julianrhughes microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT anammeireles microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT katherinehfisher microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT angelgarcia microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT philiprantrobus microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT alanwainman microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT nicolezitzmann microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT charlottedeane microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT hiroyukiohkura microtubuleinteractomecomplexeswithrolesincellcycleandmitosis AT jamesgwakefield microtubuleinteractomecomplexeswithrolesincellcycleandmitosis
_version_	1718375925488287744

A microtubule interactome: complexes with roles in cell cycle and mitosis.

Ejemplares similares