Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.

Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a...

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Autores principales:	Eamonn P Culligan, Roy D Sleator, Julian R Marchesi, Colin Hill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4840a0fbf3d42abba2f57ec97d9c346
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spelling	oai:doaj.org-article:c4840a0fbf3d42abba2f57ec97d9c3462021-11-18T08:42:11ZFunctional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.1932-620310.1371/journal.pone.0082985https://doaj.org/article/c4840a0fbf3d42abba2f57ec97d9c3462013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349412/?tool=EBIhttps://doaj.org/toc/1932-6203Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment.Eamonn P CulliganRoy D SleatorJulian R MarchesiColin HillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82985 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eamonn P Culligan Roy D Sleator Julian R Marchesi Colin Hill Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
description	Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment.
format	article
author	Eamonn P Culligan Roy D Sleator Julian R Marchesi Colin Hill
author_facet	Eamonn P Culligan Roy D Sleator Julian R Marchesi Colin Hill
author_sort	Eamonn P Culligan
title	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
title_short	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
title_full	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
title_fullStr	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
title_full_unstemmed	Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.
title_sort	functional environmental screening of a metagenomic library identifies stla; a unique salt tolerance locus from the human gut microbiome.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c4840a0fbf3d42abba2f57ec97d9c346
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Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome.

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