Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses

Abstract Fasciola hepatica, a global worm parasite of humans and their livestock, regulates host innate immune responses within hours of infection. Host macrophages, essential to the first-line defence mechanisms, are quickly restricted in their ability to initiate a classic protective pro-inflammat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nham Tran, Alison Ricafrente, Joyce To, Maria Lund, Tania M. Marques, Margarida Gama-Carvalho, Krystyna Cwiklinski, John P. Dalton, Sheila Donnelly
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c491f3abaaee4078b8355327c7eadde9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c491f3abaaee4078b8355327c7eadde9
record_format	dspace
spelling	oai:doaj.org-article:c491f3abaaee4078b8355327c7eadde92021-12-02T14:02:53ZFasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses10.1038/s41598-021-86125-12045-2322https://doaj.org/article/c491f3abaaee4078b8355327c7eadde92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86125-1https://doaj.org/toc/2045-2322Abstract Fasciola hepatica, a global worm parasite of humans and their livestock, regulates host innate immune responses within hours of infection. Host macrophages, essential to the first-line defence mechanisms, are quickly restricted in their ability to initiate a classic protective pro-inflammatory immune response. We found that macrophages from infected animals are enriched with parasite-derived micro(mi)RNAs. The most abundant of these miRNAs, fhe-miR-125b, is released by the parasite via exosomes and is homologous to a mammalian miRNA, hsa-miR-125b, that is known to regulate the activation of pro-inflammatory M1 macrophages. We show that the parasite fhe-miR-125b loads onto the mammalian Argonaut protein (Ago-2) within macrophages during infection and, therefore, propose that it mimics host miR-125b to negatively regulate the production of inflammatory cytokines. The hijacking of the miRNA machinery controlling innate cell function could be a fundamental mechanism by which worm parasites disarm the early immune responses of their host to ensure successful infection.Nham TranAlison RicafrenteJoyce ToMaria LundTania M. MarquesMargarida Gama-CarvalhoKrystyna CwiklinskiJohn P. DaltonSheila DonnellyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nham Tran Alison Ricafrente Joyce To Maria Lund Tania M. Marques Margarida Gama-Carvalho Krystyna Cwiklinski John P. Dalton Sheila Donnelly Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
description	Abstract Fasciola hepatica, a global worm parasite of humans and their livestock, regulates host innate immune responses within hours of infection. Host macrophages, essential to the first-line defence mechanisms, are quickly restricted in their ability to initiate a classic protective pro-inflammatory immune response. We found that macrophages from infected animals are enriched with parasite-derived micro(mi)RNAs. The most abundant of these miRNAs, fhe-miR-125b, is released by the parasite via exosomes and is homologous to a mammalian miRNA, hsa-miR-125b, that is known to regulate the activation of pro-inflammatory M1 macrophages. We show that the parasite fhe-miR-125b loads onto the mammalian Argonaut protein (Ago-2) within macrophages during infection and, therefore, propose that it mimics host miR-125b to negatively regulate the production of inflammatory cytokines. The hijacking of the miRNA machinery controlling innate cell function could be a fundamental mechanism by which worm parasites disarm the early immune responses of their host to ensure successful infection.
format	article
author	Nham Tran Alison Ricafrente Joyce To Maria Lund Tania M. Marques Margarida Gama-Carvalho Krystyna Cwiklinski John P. Dalton Sheila Donnelly
author_facet	Nham Tran Alison Ricafrente Joyce To Maria Lund Tania M. Marques Margarida Gama-Carvalho Krystyna Cwiklinski John P. Dalton Sheila Donnelly
author_sort	Nham Tran
title	Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
title_short	Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
title_full	Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
title_fullStr	Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
title_full_unstemmed	Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses
title_sort	fasciola hepatica hijacks host macrophage mirna machinery to modulate early innate immune responses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c491f3abaaee4078b8355327c7eadde9
work_keys_str_mv	AT nhamtran fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT alisonricafrente fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT joyceto fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT marialund fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT taniammarques fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT margaridagamacarvalho fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT krystynacwiklinski fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT johnpdalton fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses AT sheiladonnelly fasciolahepaticahijackshostmacrophagemirnamachinerytomodulateearlyinnateimmuneresponses
_version_	1718392107580784640

Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses

Ejemplares similares