Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR

Black ginseng exhibits numerous pharmacological activities due to higher and more diverse ginsenosides than unprocessed white ginseng. The ginsenoside derivatives have been investigated in order to determine their chemical structures and pharmacological activities. We found a peak which was increase...

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Autores principales:	Tae-Kyung Kim, Changsuk Lee, Taek-Hee Nam, Yong-Ki Seo, Kyeong-Soo Kim, Kimoon Kang, Seok-Hun Yun, Seok-Hwan Yoon, Jaeho Jeong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red ginseng black ginseng linoleic acid mass spectrometry nuclear magnetic resonance spectroscopy Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c493566779d740608019c364e571619c
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spelling	oai:doaj.org-article:c493566779d740608019c364e571619c2021-11-25T18:59:22ZIsolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR10.3390/separations81102082297-8739https://doaj.org/article/c493566779d740608019c364e571619c2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/208https://doaj.org/toc/2297-8739Black ginseng exhibits numerous pharmacological activities due to higher and more diverse ginsenosides than unprocessed white ginseng. The ginsenoside derivatives have been investigated in order to determine their chemical structures and pharmacological activities. We found a peak which was increased 10-fold but unidentified in the methanol extracts of a black ginseng product. The unknown peak was tracked and identified as linoleic acid rather than a ginsenoside derivative using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. NMR analysis confirmed no presence of conjugated linoleic acids. Ginsenoside profiles and linoleic acid contents in black ginseng products were quantified using LC-MS/MS. Linoleic acid content was more directly proportional to the number of applied thermal cycles in the manufacturing process than any ginsenosides.Tae-Kyung KimChangsuk LeeTaek-Hee NamYong-Ki SeoKyeong-Soo KimKimoon KangSeok-Hun YunSeok-Hwan YoonJaeho JeongMDPI AGarticlered ginsengblack ginsenglinoleic acidmass spectrometrynuclear magnetic resonance spectroscopyPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 208, p 208 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red ginseng black ginseng linoleic acid mass spectrometry nuclear magnetic resonance spectroscopy Physics QC1-999 Chemistry QD1-999
spellingShingle	red ginseng black ginseng linoleic acid mass spectrometry nuclear magnetic resonance spectroscopy Physics QC1-999 Chemistry QD1-999 Tae-Kyung Kim Changsuk Lee Taek-Hee Nam Yong-Ki Seo Kyeong-Soo Kim Kimoon Kang Seok-Hun Yun Seok-Hwan Yoon Jaeho Jeong Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
description	Black ginseng exhibits numerous pharmacological activities due to higher and more diverse ginsenosides than unprocessed white ginseng. The ginsenoside derivatives have been investigated in order to determine their chemical structures and pharmacological activities. We found a peak which was increased 10-fold but unidentified in the methanol extracts of a black ginseng product. The unknown peak was tracked and identified as linoleic acid rather than a ginsenoside derivative using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. NMR analysis confirmed no presence of conjugated linoleic acids. Ginsenoside profiles and linoleic acid contents in black ginseng products were quantified using LC-MS/MS. Linoleic acid content was more directly proportional to the number of applied thermal cycles in the manufacturing process than any ginsenosides.
format	article
author	Tae-Kyung Kim Changsuk Lee Taek-Hee Nam Yong-Ki Seo Kyeong-Soo Kim Kimoon Kang Seok-Hun Yun Seok-Hwan Yoon Jaeho Jeong
author_facet	Tae-Kyung Kim Changsuk Lee Taek-Hee Nam Yong-Ki Seo Kyeong-Soo Kim Kimoon Kang Seok-Hun Yun Seok-Hwan Yoon Jaeho Jeong
author_sort	Tae-Kyung Kim
title	Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
title_short	Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
title_full	Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
title_fullStr	Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
title_full_unstemmed	Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR
title_sort	isolation and identification of non-conjugated linoleic acid from processed <i>panax ginseng</i> using lc-ms/ms and <sup>1</sup>h-nmr
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c493566779d740608019c364e571619c
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Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR

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