Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses

Limb wounds are common in horses and often develop complications. Intravenous multipotent mesenchymal stromal cell (MSC) therapy is promising but has risks associated with intravenous administration and unknown potential to improve cutaneous wound healing. The objectives were to determine the clinic...

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Autores principales:	Suzanne J. K. Mund, Daniel J. MacPhee, John Campbell, Ali Honaramooz, Bruce Wobeser, Spencer M. Barber
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	stem cells cytokines wound healing fibrosis exuberant granulation tissue equine Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c496adf2b90d4a54a44cbca6415c2c78
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spelling	oai:doaj.org-article:c496adf2b90d4a54a44cbca6415c2c782021-11-25T17:09:56ZMacroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses10.3390/cells101129722073-4409https://doaj.org/article/c496adf2b90d4a54a44cbca6415c2c782021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2972https://doaj.org/toc/2073-4409Limb wounds are common in horses and often develop complications. Intravenous multipotent mesenchymal stromal cell (MSC) therapy is promising but has risks associated with intravenous administration and unknown potential to improve cutaneous wound healing. The objectives were to determine the clinical safety of administering large numbers of allogeneic cord blood-derived MSCs intravenously, and if therapy causes clinically adverse reactions, accelerates wound closure, improves histologic healing, and alters mRNA expression of common wound cytokines. Wounds were created on the metacarpus of 12 horses. Treatment horses were administered 1.51–2.46 × 10<sup>8</sup> cells suspended in 50% HypoThermosol FRS, and control horses were administered 50% HypoThermosol FRS alone. Epithelialization, contraction, and wound closure rates were determined using planimetric analysis. Wounds were biopsied and evaluated for histologic healing characteristics and cytokine mRNA expression. Days until wound closure was also determined. The results indicate that 3/6 of treatment horses and 1/6 of control horses experienced minor transient reactions. Treatment did not accelerate wound closure or improve histologic healing. Treatment decreased wound size and decreased all measured cytokines except transforming growth factor-β3. MSC intravenous therapy has the potential to decrease limb wound size; however, further work is needed to understand the clinical relevance of adverse reactions.Suzanne J. K. MundDaniel J. MacPheeJohn CampbellAli HonaramoozBruce WobeserSpencer M. BarberMDPI AGarticlestem cellscytokineswound healingfibrosisexuberant granulation tissueequineBiology (General)QH301-705.5ENCells, Vol 10, Iss 2972, p 2972 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stem cells cytokines wound healing fibrosis exuberant granulation tissue equine Biology (General) QH301-705.5
spellingShingle	stem cells cytokines wound healing fibrosis exuberant granulation tissue equine Biology (General) QH301-705.5 Suzanne J. K. Mund Daniel J. MacPhee John Campbell Ali Honaramooz Bruce Wobeser Spencer M. Barber Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
description	Limb wounds are common in horses and often develop complications. Intravenous multipotent mesenchymal stromal cell (MSC) therapy is promising but has risks associated with intravenous administration and unknown potential to improve cutaneous wound healing. The objectives were to determine the clinical safety of administering large numbers of allogeneic cord blood-derived MSCs intravenously, and if therapy causes clinically adverse reactions, accelerates wound closure, improves histologic healing, and alters mRNA expression of common wound cytokines. Wounds were created on the metacarpus of 12 horses. Treatment horses were administered 1.51–2.46 × 10<sup>8</sup> cells suspended in 50% HypoThermosol FRS, and control horses were administered 50% HypoThermosol FRS alone. Epithelialization, contraction, and wound closure rates were determined using planimetric analysis. Wounds were biopsied and evaluated for histologic healing characteristics and cytokine mRNA expression. Days until wound closure was also determined. The results indicate that 3/6 of treatment horses and 1/6 of control horses experienced minor transient reactions. Treatment did not accelerate wound closure or improve histologic healing. Treatment decreased wound size and decreased all measured cytokines except transforming growth factor-β3. MSC intravenous therapy has the potential to decrease limb wound size; however, further work is needed to understand the clinical relevance of adverse reactions.
format	article
author	Suzanne J. K. Mund Daniel J. MacPhee John Campbell Ali Honaramooz Bruce Wobeser Spencer M. Barber
author_facet	Suzanne J. K. Mund Daniel J. MacPhee John Campbell Ali Honaramooz Bruce Wobeser Spencer M. Barber
author_sort	Suzanne J. K. Mund
title	Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
title_short	Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
title_full	Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
title_fullStr	Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
title_full_unstemmed	Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses
title_sort	macroscopic, histologic, and immunomodulatory response of limb wounds following intravenous allogeneic cord blood-derived multipotent mesenchymal stromal cell therapy in horses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c496adf2b90d4a54a44cbca6415c2c78
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Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses

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