In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles

Abstract Capable of generating plasmonic and other effects, gold nanostructures can offer a variety of diagnostic and therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements for toxicity levels and surfa...

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Autores principales:	Anne-Laure Bailly, Florian Correard, Anton Popov, Gleb Tselikov, Florence Chaspoul, Romain Appay, Ahmed Al-Kattan, Andrei V. Kabashin, Diane Braguer, Marie-Anne Esteve
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4a199e1faa94f019304bc64197a408a
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spelling	oai:doaj.org-article:c4a199e1faa94f019304bc64197a408a2021-12-02T15:09:53ZIn vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles10.1038/s41598-019-48748-32045-2322https://doaj.org/article/c4a199e1faa94f019304bc64197a408a2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48748-3https://doaj.org/toc/2045-2322Abstract Capable of generating plasmonic and other effects, gold nanostructures can offer a variety of diagnostic and therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements for toxicity levels and surface conditioning. Laser-synthesized Au nanoparticles (AuNP) present a viable alternative to chemical counterparts and can offer exceptional purity (no trace of contaminants) and unusual surface chemistry making possible direct conjugation with biocompatible polymers (dextran, polyethylene glycol). This work presents the first pharmacokinetics, biodistribution and safety study of laser-ablated dextran-coated AuNP (AuNPd) under intravenous administration in small animal model. Our data show that AuNPd are rapidly eliminated from the blood circulation and accumulated preferentially in liver and spleen, without inducing liver or kidney toxicity, as confirmed by the plasmatic ALAT and ASAT activities, and creatininemia values. Despite certain residual accumulation in tissues, we did not detect any sign of histological damage or inflammation in tissues, while IL-6 level confirmed the absence of any chronic inflammation. The safety of AuNPd was confirmed by healthy behavior of animals and the absence of acute and chronic toxicities in liver, spleen and kidneys. Our results demonstrate that laser-synthesized AuNP are safe for biological systems, which promises their successful biomedical applications.Anne-Laure BaillyFlorian CorreardAnton PopovGleb TselikovFlorence ChaspoulRomain AppayAhmed Al-KattanAndrei V. KabashinDiane BraguerMarie-Anne EsteveNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne-Laure Bailly Florian Correard Anton Popov Gleb Tselikov Florence Chaspoul Romain Appay Ahmed Al-Kattan Andrei V. Kabashin Diane Braguer Marie-Anne Esteve In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
description	Abstract Capable of generating plasmonic and other effects, gold nanostructures can offer a variety of diagnostic and therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements for toxicity levels and surface conditioning. Laser-synthesized Au nanoparticles (AuNP) present a viable alternative to chemical counterparts and can offer exceptional purity (no trace of contaminants) and unusual surface chemistry making possible direct conjugation with biocompatible polymers (dextran, polyethylene glycol). This work presents the first pharmacokinetics, biodistribution and safety study of laser-ablated dextran-coated AuNP (AuNPd) under intravenous administration in small animal model. Our data show that AuNPd are rapidly eliminated from the blood circulation and accumulated preferentially in liver and spleen, without inducing liver or kidney toxicity, as confirmed by the plasmatic ALAT and ASAT activities, and creatininemia values. Despite certain residual accumulation in tissues, we did not detect any sign of histological damage or inflammation in tissues, while IL-6 level confirmed the absence of any chronic inflammation. The safety of AuNPd was confirmed by healthy behavior of animals and the absence of acute and chronic toxicities in liver, spleen and kidneys. Our results demonstrate that laser-synthesized AuNP are safe for biological systems, which promises their successful biomedical applications.
format	article
author	Anne-Laure Bailly Florian Correard Anton Popov Gleb Tselikov Florence Chaspoul Romain Appay Ahmed Al-Kattan Andrei V. Kabashin Diane Braguer Marie-Anne Esteve
author_facet	Anne-Laure Bailly Florian Correard Anton Popov Gleb Tselikov Florence Chaspoul Romain Appay Ahmed Al-Kattan Andrei V. Kabashin Diane Braguer Marie-Anne Esteve
author_sort	Anne-Laure Bailly
title	In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
title_short	In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
title_full	In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
title_fullStr	In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
title_full_unstemmed	In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
title_sort	in vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c4a199e1faa94f019304bc64197a408a
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In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles

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