Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.

Studies of female genital structures have generally lagged behind comparable studies of male genitalia, in part because of an assumption of a lower level of variability, but also because internal genitalia are much more difficult to study. Using multiple microscopy techniques, including video stereo...

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Autores principales:	Orlando Cómbita-Heredia, Connor J Gulbronson, Ronald Ochoa, Edwin Javier Quintero-Gutiérrez, Gary Bauchan, Hans Klompen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4b56458277e43c2b98dd6e95827cdb6
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spelling	oai:doaj.org-article:c4b56458277e43c2b98dd6e95827cdb62021-12-02T20:15:00ZSize, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.1932-620310.1371/journal.pone.0254974https://doaj.org/article/c4b56458277e43c2b98dd6e95827cdb62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254974https://doaj.org/toc/1932-6203Studies of female genital structures have generally lagged behind comparable studies of male genitalia, in part because of an assumption of a lower level of variability, but also because internal genitalia are much more difficult to study. Using multiple microscopy techniques, including video stereomicroscopy, fluorescence microscopy, low-temperature scanning electron microscopy (LT-SEM), and confocal laser scanning microscopy (CLSM) we examined whether the complex sperm transfer structures in males of Megalolaelaps colossus (Acari: Mesostigmata) are matched by similarly complex internal structures in the female. While both LT-SEM and CLSM are well suited for obtaining high-quality surface images, CLSM also proved to be a valuable technique for observing internal anatomical structures. The long and coiled sperm transfer organ on the chelicera of the males (spermatodactyl) largely matches an equally complex, but internal, spiral structure in the females in shape, size, and direction. This result strongly suggests some form of genital coevolution. A hypothesis of sexual conflict appears to provide the best fit for all available data (morphology and life history).Orlando Cómbita-HerediaConnor J GulbronsonRonald OchoaEdwin Javier Quintero-GutiérrezGary BauchanHans KlompenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254974 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Orlando Cómbita-Heredia Connor J Gulbronson Ronald Ochoa Edwin Javier Quintero-Gutiérrez Gary Bauchan Hans Klompen Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
description	Studies of female genital structures have generally lagged behind comparable studies of male genitalia, in part because of an assumption of a lower level of variability, but also because internal genitalia are much more difficult to study. Using multiple microscopy techniques, including video stereomicroscopy, fluorescence microscopy, low-temperature scanning electron microscopy (LT-SEM), and confocal laser scanning microscopy (CLSM) we examined whether the complex sperm transfer structures in males of Megalolaelaps colossus (Acari: Mesostigmata) are matched by similarly complex internal structures in the female. While both LT-SEM and CLSM are well suited for obtaining high-quality surface images, CLSM also proved to be a valuable technique for observing internal anatomical structures. The long and coiled sperm transfer organ on the chelicera of the males (spermatodactyl) largely matches an equally complex, but internal, spiral structure in the females in shape, size, and direction. This result strongly suggests some form of genital coevolution. A hypothesis of sexual conflict appears to provide the best fit for all available data (morphology and life history).
format	article
author	Orlando Cómbita-Heredia Connor J Gulbronson Ronald Ochoa Edwin Javier Quintero-Gutiérrez Gary Bauchan Hans Klompen
author_facet	Orlando Cómbita-Heredia Connor J Gulbronson Ronald Ochoa Edwin Javier Quintero-Gutiérrez Gary Bauchan Hans Klompen
author_sort	Orlando Cómbita-Heredia
title	Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
title_short	Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
title_full	Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
title_fullStr	Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
title_full_unstemmed	Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.
title_sort	size, shape, and direction matters: matching secondary genital structures in male and female mites using multiple microscopy techniques and 3d modeling.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c4b56458277e43c2b98dd6e95827cdb6
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Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling.

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