Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats

Existing studies show that CNS oxytocin (OT) signaling is important in the control of energy balance, but it is unclear which neurons may contribute to these effects. Our goals were to examine (1) the dose-response effects of acute OT administration into the third (3V; forebrain) and fourth (4V; hin...

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Autores principales:	Vishwanath T. Anekonda, Benjamin W. Thompson, Jacqueline M. Ho, Zachary S. Roberts, Melise M. Edwards, Ha K. Nguyen, Andrew D. Dodson, Tami Wolden-Hanson, Daniel W. Chukri, Adam J. Herbertson, James L. Graham, Peter J. Havel, Tomasz A. Wietecha, Kevin D. O’Brien, James E. Blevins
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	satiety meal size oxytocin hindbrain Medicine R
Acceso en línea:	https://doaj.org/article/c4b875872ecd4b8ca5893429a933acdb
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spelling	oai:doaj.org-article:c4b875872ecd4b8ca5893429a933acdb2021-11-11T17:41:57ZHindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats10.3390/jcm102150782077-0383https://doaj.org/article/c4b875872ecd4b8ca5893429a933acdb2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5078https://doaj.org/toc/2077-0383Existing studies show that CNS oxytocin (OT) signaling is important in the control of energy balance, but it is unclear which neurons may contribute to these effects. Our goals were to examine (1) the dose-response effects of acute OT administration into the third (3V; forebrain) and fourth (4V; hindbrain) ventricles to assess sensitivity to OT in forebrain and hindbrain sites, (2) the extent to which chronic 4V administration of OT reduces weight gain associated with the progression of diet-induced obesity, and (3) whether nucleus tractus solitarius (NTS) catecholamine neurons are downstream targets of 4V OT. Initially, we examined the dose-response effects of 3V and 4V OT (0.04, 0.2, 1, or 5 μg). 3V and 4V OT (5 μg) suppressed 0.5-h food intake by 71.7 ± 6.0% and 60 ± 12.9%, respectively. 4V OT (0.04, 0.2, 1 μg) reduced food intake by 30.9 ± 12.9, 42.1 ± 9.4, and 56.4 ± 9.0%, respectively, whereas 3V administration of OT (1 μg) was only effective at reducing 0.5-h food intake by 38.3 ± 10.9%. We subsequently found that chronic 4V OT infusion, as with chronic 3V infusion, reduced body weight gain (specific to fat mass) and tended to reduce plasma leptin in high-fat diet (HFD)-fed rats, in part, through a reduction in energy intake. Lastly, we determined that 4V OT increased the number of hindbrain caudal NTS Fos (+) neurons (156 ± 25) relative to vehicle (12 ± 3). The 4V OT also induced Fos in tyrosine hydroxylase (TH; marker of catecholamine neurons) (+) neurons (25 ± 7%) relative to vehicle (0.8 ± 0.3%). Collectively, these findings support the hypothesis that OT within the hindbrain is effective at reducing food intake, weight gain, and adiposity and that NTS catecholamine neurons in addition to non-catecholaminergic neurons are downstream targets of CNS OT.Vishwanath T. AnekondaBenjamin W. ThompsonJacqueline M. HoZachary S. RobertsMelise M. EdwardsHa K. NguyenAndrew D. DodsonTami Wolden-HansonDaniel W. ChukriAdam J. HerbertsonJames L. GrahamPeter J. HavelTomasz A. WietechaKevin D. O’BrienJames E. BlevinsMDPI AGarticlesatietymeal sizeoxytocinhindbrainMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5078, p 5078 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	satiety meal size oxytocin hindbrain Medicine R
spellingShingle	satiety meal size oxytocin hindbrain Medicine R Vishwanath T. Anekonda Benjamin W. Thompson Jacqueline M. Ho Zachary S. Roberts Melise M. Edwards Ha K. Nguyen Andrew D. Dodson Tami Wolden-Hanson Daniel W. Chukri Adam J. Herbertson James L. Graham Peter J. Havel Tomasz A. Wietecha Kevin D. O’Brien James E. Blevins Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
description	Existing studies show that CNS oxytocin (OT) signaling is important in the control of energy balance, but it is unclear which neurons may contribute to these effects. Our goals were to examine (1) the dose-response effects of acute OT administration into the third (3V; forebrain) and fourth (4V; hindbrain) ventricles to assess sensitivity to OT in forebrain and hindbrain sites, (2) the extent to which chronic 4V administration of OT reduces weight gain associated with the progression of diet-induced obesity, and (3) whether nucleus tractus solitarius (NTS) catecholamine neurons are downstream targets of 4V OT. Initially, we examined the dose-response effects of 3V and 4V OT (0.04, 0.2, 1, or 5 μg). 3V and 4V OT (5 μg) suppressed 0.5-h food intake by 71.7 ± 6.0% and 60 ± 12.9%, respectively. 4V OT (0.04, 0.2, 1 μg) reduced food intake by 30.9 ± 12.9, 42.1 ± 9.4, and 56.4 ± 9.0%, respectively, whereas 3V administration of OT (1 μg) was only effective at reducing 0.5-h food intake by 38.3 ± 10.9%. We subsequently found that chronic 4V OT infusion, as with chronic 3V infusion, reduced body weight gain (specific to fat mass) and tended to reduce plasma leptin in high-fat diet (HFD)-fed rats, in part, through a reduction in energy intake. Lastly, we determined that 4V OT increased the number of hindbrain caudal NTS Fos (+) neurons (156 ± 25) relative to vehicle (12 ± 3). The 4V OT also induced Fos in tyrosine hydroxylase (TH; marker of catecholamine neurons) (+) neurons (25 ± 7%) relative to vehicle (0.8 ± 0.3%). Collectively, these findings support the hypothesis that OT within the hindbrain is effective at reducing food intake, weight gain, and adiposity and that NTS catecholamine neurons in addition to non-catecholaminergic neurons are downstream targets of CNS OT.
format	article
author	Vishwanath T. Anekonda Benjamin W. Thompson Jacqueline M. Ho Zachary S. Roberts Melise M. Edwards Ha K. Nguyen Andrew D. Dodson Tami Wolden-Hanson Daniel W. Chukri Adam J. Herbertson James L. Graham Peter J. Havel Tomasz A. Wietecha Kevin D. O’Brien James E. Blevins
author_facet	Vishwanath T. Anekonda Benjamin W. Thompson Jacqueline M. Ho Zachary S. Roberts Melise M. Edwards Ha K. Nguyen Andrew D. Dodson Tami Wolden-Hanson Daniel W. Chukri Adam J. Herbertson James L. Graham Peter J. Havel Tomasz A. Wietecha Kevin D. O’Brien James E. Blevins
author_sort	Vishwanath T. Anekonda
title	Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
title_short	Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
title_full	Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
title_fullStr	Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
title_full_unstemmed	Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats
title_sort	hindbrain administration of oxytocin reduces food intake, weight gain and activates catecholamine neurons in the hindbrain nucleus of the solitary tract in rats
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c4b875872ecd4b8ca5893429a933acdb
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Hindbrain Administration of Oxytocin Reduces Food Intake, Weight Gain and Activates Catecholamine Neurons in the Hindbrain Nucleus of the Solitary Tract in Rats

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