Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.

In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Denis S Grouzdev, Marina V Dziuba, Denis V Kurek, Alexander I Ovchinnikov, Nadezhda A Zhigalova, Boris B Kuznetsov, Konstantin G Skryabin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4bfaff759a34201af328af66a601784
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c4bfaff759a34201af328af66a601784
record_format	dspace
spelling	oai:doaj.org-article:c4bfaff759a34201af328af66a6017842021-11-25T05:56:26ZOptimized method for preparation of IgG-binding bacterial magnetic nanoparticles.1932-620310.1371/journal.pone.0109914https://doaj.org/article/c4bfaff759a34201af328af66a6017842014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109914https://doaj.org/toc/1932-6203In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.Denis S GrouzdevMarina V DziubaDenis V KurekAlexander I OvchinnikovNadezhda A ZhigalovaBoris B KuznetsovKonstantin G SkryabinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109914 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Denis S Grouzdev Marina V Dziuba Denis V Kurek Alexander I Ovchinnikov Nadezhda A Zhigalova Boris B Kuznetsov Konstantin G Skryabin Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
description	In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.
format	article
author	Denis S Grouzdev Marina V Dziuba Denis V Kurek Alexander I Ovchinnikov Nadezhda A Zhigalova Boris B Kuznetsov Konstantin G Skryabin
author_facet	Denis S Grouzdev Marina V Dziuba Denis V Kurek Alexander I Ovchinnikov Nadezhda A Zhigalova Boris B Kuznetsov Konstantin G Skryabin
author_sort	Denis S Grouzdev
title	Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
title_short	Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
title_full	Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
title_fullStr	Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
title_full_unstemmed	Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.
title_sort	optimized method for preparation of igg-binding bacterial magnetic nanoparticles.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c4bfaff759a34201af328af66a601784
work_keys_str_mv	AT denissgrouzdev optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT marinavdziuba optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT denisvkurek optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT alexanderiovchinnikov optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT nadezhdaazhigalova optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT borisbkuznetsov optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles AT konstantingskryabin optimizedmethodforpreparationofiggbindingbacterialmagneticnanoparticles
_version_	1718414366349459456

Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.

Ejemplares similares