Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort
Abstract We examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self...
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Nature Portfolio
2017
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oai:doaj.org-article:c4c22f56af6e451cbed80f3da20d02082021-12-02T15:04:56ZDetermination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort10.1038/s41598-017-01212-62045-2322https://doaj.org/article/c4c22f56af6e451cbed80f3da20d02082017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01212-6https://doaj.org/toc/2045-2322Abstract We examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants’ satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1–V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.Manas K. AkmatovNadine KochMarius VitalWolfgang AhrensDieter Flesch-JanysJulia FrickeAnja GatzemeierHalina GreiserKathrin GüntherThomas IlligRudolf KaaksBastian KroneAndrea KühnJakob LinseisenChristine MeisingerKarin MichelsSusanne MoebusAlexandra NietersNadia ObiAnja SchultzeJulia Six-MerkerDietmar H. PieperFrank PesslerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Manas K. Akmatov Nadine Koch Marius Vital Wolfgang Ahrens Dieter Flesch-Janys Julia Fricke Anja Gatzemeier Halina Greiser Kathrin Günther Thomas Illig Rudolf Kaaks Bastian Krone Andrea Kühn Jakob Linseisen Christine Meisinger Karin Michels Susanne Moebus Alexandra Nieters Nadia Obi Anja Schultze Julia Six-Merker Dietmar H. Pieper Frank Pessler Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| description |
Abstract We examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants’ satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1–V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken. |
| format |
article |
| author |
Manas K. Akmatov Nadine Koch Marius Vital Wolfgang Ahrens Dieter Flesch-Janys Julia Fricke Anja Gatzemeier Halina Greiser Kathrin Günther Thomas Illig Rudolf Kaaks Bastian Krone Andrea Kühn Jakob Linseisen Christine Meisinger Karin Michels Susanne Moebus Alexandra Nieters Nadia Obi Anja Schultze Julia Six-Merker Dietmar H. Pieper Frank Pessler |
| author_facet |
Manas K. Akmatov Nadine Koch Marius Vital Wolfgang Ahrens Dieter Flesch-Janys Julia Fricke Anja Gatzemeier Halina Greiser Kathrin Günther Thomas Illig Rudolf Kaaks Bastian Krone Andrea Kühn Jakob Linseisen Christine Meisinger Karin Michels Susanne Moebus Alexandra Nieters Nadia Obi Anja Schultze Julia Six-Merker Dietmar H. Pieper Frank Pessler |
| author_sort |
Manas K. Akmatov |
| title |
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| title_short |
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| title_full |
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| title_fullStr |
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| title_full_unstemmed |
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from Pretest 1 of the German National Cohort |
| title_sort |
determination of nasal and oropharyngeal microbiomes in a multicenter population-based study – findings from pretest 1 of the german national cohort |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/c4c22f56af6e451cbed80f3da20d0208 |
| work_keys_str_mv |
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