Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes
Abstract Carcinogenic effect of tobacco in oral cancer is through chewing and/or smoking. Significant differences exist in development of oral cancer between tobacco users and non-users. However, molecular alterations induced by different forms of tobacco are yet to be fully elucidated. We developed...
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2018
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oai:doaj.org-article:c4c56ad91ce94c10877b609c906321342021-12-02T15:07:55ZCigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes10.1038/s41598-018-25498-22045-2322https://doaj.org/article/c4c56ad91ce94c10877b609c906321342018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25498-2https://doaj.org/toc/2045-2322Abstract Carcinogenic effect of tobacco in oral cancer is through chewing and/or smoking. Significant differences exist in development of oral cancer between tobacco users and non-users. However, molecular alterations induced by different forms of tobacco are yet to be fully elucidated. We developed cellular models of chronic exposure to chewing tobacco and cigarette smoke using immortalized oral keratinocytes. Chronic exposure to tobacco resulted in increased cell scattering and invasiveness in immortalized oral keratinocytes. miRNA sequencing using Illumina HiSeq 2500 resulted in the identification of 10 significantly dysregulated miRNAs (4 fold; p ≤ 0.05) in chewing tobacco treated cells and 6 in cigarette smoke exposed cells. We integrated this data with global proteomic data and identified 36 protein targets that showed inverse expression pattern in chewing tobacco treated cells and 16 protein targets that showed inverse expression in smoke exposed cells. In addition, we identified 6 novel miRNAs in chewing tobacco treated cells and 18 novel miRNAs in smoke exposed cells. Integrative analysis of dysregulated miRNAs and their targets indicates that signaling mechanisms leading to oncogenic transformation are distinct between both forms of tobacco. Our study demonstrates alterations in miRNA expression in oral cells in response to two frequently used forms of tobacco.Mohd Younis BhatJayshree AdvaniPavithra RajagopalanKrishna PatelVishalakshi NanjappaHitendra S. SolankiArun H. PatilFirdous A. BhatPremendu P. MathurBipin NairT. S. Keshava PrasadJoseph A. CalifanoDavid SidranskyHarsha GowdaAditi ChatterjeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
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Medicine R Science Q Mohd Younis Bhat Jayshree Advani Pavithra Rajagopalan Krishna Patel Vishalakshi Nanjappa Hitendra S. Solanki Arun H. Patil Firdous A. Bhat Premendu P. Mathur Bipin Nair T. S. Keshava Prasad Joseph A. Califano David Sidransky Harsha Gowda Aditi Chatterjee Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| description |
Abstract Carcinogenic effect of tobacco in oral cancer is through chewing and/or smoking. Significant differences exist in development of oral cancer between tobacco users and non-users. However, molecular alterations induced by different forms of tobacco are yet to be fully elucidated. We developed cellular models of chronic exposure to chewing tobacco and cigarette smoke using immortalized oral keratinocytes. Chronic exposure to tobacco resulted in increased cell scattering and invasiveness in immortalized oral keratinocytes. miRNA sequencing using Illumina HiSeq 2500 resulted in the identification of 10 significantly dysregulated miRNAs (4 fold; p ≤ 0.05) in chewing tobacco treated cells and 6 in cigarette smoke exposed cells. We integrated this data with global proteomic data and identified 36 protein targets that showed inverse expression pattern in chewing tobacco treated cells and 16 protein targets that showed inverse expression in smoke exposed cells. In addition, we identified 6 novel miRNAs in chewing tobacco treated cells and 18 novel miRNAs in smoke exposed cells. Integrative analysis of dysregulated miRNAs and their targets indicates that signaling mechanisms leading to oncogenic transformation are distinct between both forms of tobacco. Our study demonstrates alterations in miRNA expression in oral cells in response to two frequently used forms of tobacco. |
| format |
article |
| author |
Mohd Younis Bhat Jayshree Advani Pavithra Rajagopalan Krishna Patel Vishalakshi Nanjappa Hitendra S. Solanki Arun H. Patil Firdous A. Bhat Premendu P. Mathur Bipin Nair T. S. Keshava Prasad Joseph A. Califano David Sidransky Harsha Gowda Aditi Chatterjee |
| author_facet |
Mohd Younis Bhat Jayshree Advani Pavithra Rajagopalan Krishna Patel Vishalakshi Nanjappa Hitendra S. Solanki Arun H. Patil Firdous A. Bhat Premendu P. Mathur Bipin Nair T. S. Keshava Prasad Joseph A. Califano David Sidransky Harsha Gowda Aditi Chatterjee |
| author_sort |
Mohd Younis Bhat |
| title |
Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| title_short |
Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| title_full |
Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| title_fullStr |
Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| title_full_unstemmed |
Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes |
| title_sort |
cigarette smoke and chewing tobacco alter expression of different sets of mirnas in oral keratinocytes |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/c4c56ad91ce94c10877b609c90632134 |
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