A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis

Abstract Label-free optical biosensors have received tremendous attention in point-of-care testing, especially in the emerging pandemic, COVID-19, since they advance toward early-detection, rapid, real-time, ease-of-use, and low-cost paradigms. Protein biomarkers testings require less sample modific...

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Autores principales:	Noori Kim, Kyungsup Han, Pei-Chen Su, Insup Kim, Yong-Jin Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4ce30494eb74b0baa04188e716f0aad
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spelling	oai:doaj.org-article:c4ce30494eb74b0baa04188e716f0aad2021-12-02T17:14:58ZA rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis10.1038/s41598-021-88647-02045-2322https://doaj.org/article/c4ce30494eb74b0baa04188e716f0aad2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88647-0https://doaj.org/toc/2045-2322Abstract Label-free optical biosensors have received tremendous attention in point-of-care testing, especially in the emerging pandemic, COVID-19, since they advance toward early-detection, rapid, real-time, ease-of-use, and low-cost paradigms. Protein biomarkers testings require less sample modification process compared to nucleic-acid biomarkers’. However, challenges always are in detecting low-concentration for early-stage diagnosis. Here we present a Rotationally Focused Flow (RFF) method to enhance sensitivity(wavelength shift) of label-free optical sensors by increasing the detection probability of protein-based molecules. The RFF is structured by adding a less-dense fluid to focus the target-fluid in a T-shaped microchannel. It is integrated with label-free silicon microring resonators interacting with biotin-streptavidin. The suggested mechanism has demonstrated 0.19 fM concentration detection along with a significant magnitudes sensitivity enhancement compared to single flow methods. Verified by both CFD simulations and fluorescent flow-experiments, this study provides a promising proof-of-concept platform for next-generation lab-on-a-chip bioanalytics such as ultrafast and early-detection of COVID-19.Noori KimKyungsup HanPei-Chen SuInsup KimYong-Jin YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noori Kim Kyungsup Han Pei-Chen Su Insup Kim Yong-Jin Yoon A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
description	Abstract Label-free optical biosensors have received tremendous attention in point-of-care testing, especially in the emerging pandemic, COVID-19, since they advance toward early-detection, rapid, real-time, ease-of-use, and low-cost paradigms. Protein biomarkers testings require less sample modification process compared to nucleic-acid biomarkers’. However, challenges always are in detecting low-concentration for early-stage diagnosis. Here we present a Rotationally Focused Flow (RFF) method to enhance sensitivity(wavelength shift) of label-free optical sensors by increasing the detection probability of protein-based molecules. The RFF is structured by adding a less-dense fluid to focus the target-fluid in a T-shaped microchannel. It is integrated with label-free silicon microring resonators interacting with biotin-streptavidin. The suggested mechanism has demonstrated 0.19 fM concentration detection along with a significant magnitudes sensitivity enhancement compared to single flow methods. Verified by both CFD simulations and fluorescent flow-experiments, this study provides a promising proof-of-concept platform for next-generation lab-on-a-chip bioanalytics such as ultrafast and early-detection of COVID-19.
format	article
author	Noori Kim Kyungsup Han Pei-Chen Su Insup Kim Yong-Jin Yoon
author_facet	Noori Kim Kyungsup Han Pei-Chen Su Insup Kim Yong-Jin Yoon
author_sort	Noori Kim
title	A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
title_short	A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
title_full	A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
title_fullStr	A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
title_full_unstemmed	A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis
title_sort	rotationally focused flow (rff) microfluidic biosensor by density difference for early-stage detectable diagnosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c4ce30494eb74b0baa04188e716f0aad
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A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis

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