Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels

Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be transmitted to camel, sheep, and goat. Due to the absence of anti-camel conjugat...

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Autores principales:	Ramzy H. Hamouda, Marwa F. Mahmoud
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	anticamel fitc bohv-1 dromedary ibr snt. Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.85587 https://doaj.org/article/c4d03d0dfad74e469f89722f4f939250
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spelling	oai:doaj.org-article:c4d03d0dfad74e469f89722f4f9392502021-12-02T11:43:48ZRapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camelshttps://dx.doi.org/10.21608/javs.2020.855871687-40722090-3308https://doaj.org/article/c4d03d0dfad74e469f89722f4f9392502020-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_85587.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be transmitted to camel, sheep, and goat. Due to the absence of anti-camel conjugated with fluorescein isothiocyanate (FITC) in the market, we used protein-A conjugated with FITC which binds to the Fc region of IgG of many animal species. We, therefore, prepared rabbit anti-camel IgG conjugated with FITC and compared it with protein-A conjugated with FITC to the specificity and sensitivity of these compounds in IBR detection from 35 nasal swaps in imported Egyptian dromedary camels. The sensitivity and specificity of the prepared anti-camel IgG FITC and protein-A FITC were compared using Virus Neutralization Test. The labeled protein concentration in the prepared anti-camel conjugate was 2 mg/ml which was considered as an acceptable value. The degree of labeled protein (DOL) was 5.74 cm–1M–1 and optimal DOL usually fell between 2 and 10. The titer of the prepared anti-camel IgG-FITC was 3,125. The prepared anticamel IgG–FITC and protein-A-FITC showed a sensitivity of 93.75 and 90.9%, and a specificity of 71.43% and 62.5%, respectively. Our findings show no significant difference between protein-A conjugated FITC and prepared anti-camel IgG-conjugated FITC in the rapid diagnosis of BoHV-1 in Egyptian dromedary camels.Ramzy H. HamoudaMarwa F. MahmoudEgyptian Society for Animal Managementarticleanticamel fitcbohv-1dromedaryibrsnt.ZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 2, Pp 61-66 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	anticamel fitc bohv-1 dromedary ibr snt. Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	anticamel fitc bohv-1 dromedary ibr snt. Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ramzy H. Hamouda Marwa F. Mahmoud Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
description	Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be transmitted to camel, sheep, and goat. Due to the absence of anti-camel conjugated with fluorescein isothiocyanate (FITC) in the market, we used protein-A conjugated with FITC which binds to the Fc region of IgG of many animal species. We, therefore, prepared rabbit anti-camel IgG conjugated with FITC and compared it with protein-A conjugated with FITC to the specificity and sensitivity of these compounds in IBR detection from 35 nasal swaps in imported Egyptian dromedary camels. The sensitivity and specificity of the prepared anti-camel IgG FITC and protein-A FITC were compared using Virus Neutralization Test. The labeled protein concentration in the prepared anti-camel conjugate was 2 mg/ml which was considered as an acceptable value. The degree of labeled protein (DOL) was 5.74 cm–1M–1 and optimal DOL usually fell between 2 and 10. The titer of the prepared anti-camel IgG-FITC was 3,125. The prepared anticamel IgG–FITC and protein-A-FITC showed a sensitivity of 93.75 and 90.9%, and a specificity of 71.43% and 62.5%, respectively. Our findings show no significant difference between protein-A conjugated FITC and prepared anti-camel IgG-conjugated FITC in the rapid diagnosis of BoHV-1 in Egyptian dromedary camels.
format	article
author	Ramzy H. Hamouda Marwa F. Mahmoud
author_facet	Ramzy H. Hamouda Marwa F. Mahmoud
author_sort	Ramzy H. Hamouda
title	Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
title_short	Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
title_full	Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
title_fullStr	Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
title_full_unstemmed	Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels
title_sort	rapid detection of bovine herpes virus type 1 (bohv-1) in egyptian dromedary camels
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.85587 https://doaj.org/article/c4d03d0dfad74e469f89722f4f939250
work_keys_str_mv	AT ramzyhhamouda rapiddetectionofbovineherpesvirustype1bohv1inegyptiandromedarycamels AT marwafmahmoud rapiddetectionofbovineherpesvirustype1bohv1inegyptiandromedarycamels
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Rapid Detection of Bovine Herpes Virus Type 1 (BOHV-1) in Egyptian Dromedary Camels

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