Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3

Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmenta...

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Autores principales:	Olga Radulović, Slaviša Stanković, Olja Stanojević, Zoran Vujčić, Biljana Dojnov, Milana Trifunović-Momčilov, Marija Marković
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phenol bacteria duckweed antioxidative stress Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c4d7819913f145b289987588fd0a1f11
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spelling	oai:doaj.org-article:c4d7819913f145b289987588fd0a1f112021-11-25T16:27:09ZAntioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/310.3390/antiox101117192076-3921https://doaj.org/article/c4d7819913f145b289987588fd0a1f112021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1719https://doaj.org/toc/2076-3921Duckweed (<i>L. minor</i>) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an <i>H. paralvei</i> bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with <i>H. paralvei</i> in vitro and cultivated in the presence or absence of phenol (500 mg L<sup>−1</sup>), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate <i>H. paralvei’s</i> influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in <i>L. minor</i> except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. <i>H. paralvei</i> is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.Olga RadulovićSlaviša StankovićOlja StanojevićZoran VujčićBiljana DojnovMilana Trifunović-MomčilovMarija MarkovićMDPI AGarticlephenolbacteriaduckweedantioxidativestressTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1719, p 1719 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phenol bacteria duckweed antioxidative stress Therapeutics. Pharmacology RM1-950
spellingShingle	phenol bacteria duckweed antioxidative stress Therapeutics. Pharmacology RM1-950 Olga Radulović Slaviša Stanković Olja Stanojević Zoran Vujčić Biljana Dojnov Milana Trifunović-Momčilov Marija Marković Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
description	Duckweed (<i>L. minor</i>) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an <i>H. paralvei</i> bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with <i>H. paralvei</i> in vitro and cultivated in the presence or absence of phenol (500 mg L<sup>−1</sup>), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate <i>H. paralvei’s</i> influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in <i>L. minor</i> except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. <i>H. paralvei</i> is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
format	article
author	Olga Radulović Slaviša Stanković Olja Stanojević Zoran Vujčić Biljana Dojnov Milana Trifunović-Momčilov Marija Marković
author_facet	Olga Radulović Slaviša Stanković Olja Stanojević Zoran Vujčić Biljana Dojnov Milana Trifunović-Momčilov Marija Marković
author_sort	Olga Radulović
title	Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
title_short	Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
title_full	Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
title_fullStr	Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
title_full_unstemmed	Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3
title_sort	antioxidative responses of duckweed (<i>lemna minor</i> l.) to phenol and rhizosphere-associated bacterial strain <i>hafnia paralvei</i> c32-106/3
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c4d7819913f145b289987588fd0a1f11
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Antioxidative Responses of Duckweed (<i>Lemna minor</i> L.) to Phenol and Rhizosphere-Associated Bacterial Strain <i>Hafnia paralvei</i> C32-106/3

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