Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype

Mycobacterium tuberculosis strains of different phylogenetic lineages and genetic families differ in biological properties that determine, to some extent, epidemiological features and clinical manifestation in tuberculosis (TB) patients.The aim of the study was to assess the risk of an adverse outco...

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Autores principales:	O. A. Pasechnik, A. A. Vyazovaya, M. A. Dymova, A. I. Blokh, V. L. Stasenko, M. P. Tatarintseva, I. V. Mokrousov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	mycobacterium tuberculosis genotype beijing drug resistance lethality outcome Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/c4e51f43f6ec4501b1f18591ad0b9851
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spelling	oai:doaj.org-article:c4e51f43f6ec4501b1f18591ad0b98512021-11-22T07:09:53ZTuberculosis outcomes related to the Mycobacterium tuberculosis genotype2220-76192313-739810.15789/2220-7619-2019-3-4-531-538https://doaj.org/article/c4e51f43f6ec4501b1f18591ad0b98512019-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/799https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Mycobacterium tuberculosis strains of different phylogenetic lineages and genetic families differ in biological properties that determine, to some extent, epidemiological features and clinical manifestation in tuberculosis (TB) patients.The aim of the study was to assess the risk of an adverse outcome of the disease in TB patients caused by various M. tuberculosis genotypes.Materials and methods. A total of 425 patients with respiratory TB were enrolled in this study. They were registered at phthisiatric facilities in the Omsk region from March 2015 to June 2017 period and included: males — 73.1%, mean age 39.9 years, females — 26.9%, mean age 42.0 years. M. tuberculosis culture and drug susceptibility testing and DNA extraction were performed in accordance with standard methods. Strains were assigned to the M. tuberculosis Beijing genotype and its epidemiologically relevant clusters B0/W148 and 94-32 by PCR based detection of specific markers. Non-Beijing strains were subjected to spoligotyping.Results. We found that 66.5% isolates belonged to the Beijing genotype, 12.8% — to LAM, 10.1% — to T, and 4.7% — to the Ural genotype. Multi-drug resistance (MDR) to anti-TB drugs was observed in 195 M. tuberculosis strains (45.9%). Moreover, Beijing genotype was more often isolated from patients with MDR-TB infection (PR = 2.09 (95% CI 1.6–2.74) and TB infection associated with HIV infection (PR = 1.14 (95% CI 1.01–1.31). Lethal outcome was double higher in patients infected with Beijing vs. non-Beijing strains, 28.6% vs. 14.0% (PR = 2.03; 95% CI 1.3–3.17). The risk factors were identified as follows: young age 18–44 years (RR = 1.7; 95% CI 1.18–2.7), co-morbidity with HIV (RR = 5.0; 95% CI 3.39–7.45), multiple (RR = 1.7; 95% CI 1.14–2.55) and extensive drug resistance (RR = 2.57; 95% CI 1.35–4.92), and association with the Beijing genotype (RR = 2.0, 95% CI 1.3–3.17).Conclusion. M. tuberculosis spread in the Omsk region is characterised by significant prevalence of the Beijing genotype, associated with multiple and extensive drug resistance. A significant association of adverse clinical outcomes and various factors, including association with the Beijing genotype, requires development of new approaches in the fight against tuberculosis.O. A. PasechnikA. A. VyazovayaM. A. DymovaA. I. BlokhV. L. StasenkoM. P. TatarintsevaI. V. MokrousovSankt-Peterburg : NIIÈM imeni Pasteraarticlemycobacterium tuberculosisgenotypebeijingdrug resistancelethality outcomeInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 3-4, Pp 531-538 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	mycobacterium tuberculosis genotype beijing drug resistance lethality outcome Infectious and parasitic diseases RC109-216
spellingShingle	mycobacterium tuberculosis genotype beijing drug resistance lethality outcome Infectious and parasitic diseases RC109-216 O. A. Pasechnik A. A. Vyazovaya M. A. Dymova A. I. Blokh V. L. Stasenko M. P. Tatarintseva I. V. Mokrousov Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
description	Mycobacterium tuberculosis strains of different phylogenetic lineages and genetic families differ in biological properties that determine, to some extent, epidemiological features and clinical manifestation in tuberculosis (TB) patients.The aim of the study was to assess the risk of an adverse outcome of the disease in TB patients caused by various M. tuberculosis genotypes.Materials and methods. A total of 425 patients with respiratory TB were enrolled in this study. They were registered at phthisiatric facilities in the Omsk region from March 2015 to June 2017 period and included: males — 73.1%, mean age 39.9 years, females — 26.9%, mean age 42.0 years. M. tuberculosis culture and drug susceptibility testing and DNA extraction were performed in accordance with standard methods. Strains were assigned to the M. tuberculosis Beijing genotype and its epidemiologically relevant clusters B0/W148 and 94-32 by PCR based detection of specific markers. Non-Beijing strains were subjected to spoligotyping.Results. We found that 66.5% isolates belonged to the Beijing genotype, 12.8% — to LAM, 10.1% — to T, and 4.7% — to the Ural genotype. Multi-drug resistance (MDR) to anti-TB drugs was observed in 195 M. tuberculosis strains (45.9%). Moreover, Beijing genotype was more often isolated from patients with MDR-TB infection (PR = 2.09 (95% CI 1.6–2.74) and TB infection associated with HIV infection (PR = 1.14 (95% CI 1.01–1.31). Lethal outcome was double higher in patients infected with Beijing vs. non-Beijing strains, 28.6% vs. 14.0% (PR = 2.03; 95% CI 1.3–3.17). The risk factors were identified as follows: young age 18–44 years (RR = 1.7; 95% CI 1.18–2.7), co-morbidity with HIV (RR = 5.0; 95% CI 3.39–7.45), multiple (RR = 1.7; 95% CI 1.14–2.55) and extensive drug resistance (RR = 2.57; 95% CI 1.35–4.92), and association with the Beijing genotype (RR = 2.0, 95% CI 1.3–3.17).Conclusion. M. tuberculosis spread in the Omsk region is characterised by significant prevalence of the Beijing genotype, associated with multiple and extensive drug resistance. A significant association of adverse clinical outcomes and various factors, including association with the Beijing genotype, requires development of new approaches in the fight against tuberculosis.
format	article
author	O. A. Pasechnik A. A. Vyazovaya M. A. Dymova A. I. Blokh V. L. Stasenko M. P. Tatarintseva I. V. Mokrousov
author_facet	O. A. Pasechnik A. A. Vyazovaya M. A. Dymova A. I. Blokh V. L. Stasenko M. P. Tatarintseva I. V. Mokrousov
author_sort	O. A. Pasechnik
title	Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
title_short	Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
title_full	Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
title_fullStr	Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
title_full_unstemmed	Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype
title_sort	tuberculosis outcomes related to the mycobacterium tuberculosis genotype
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/c4e51f43f6ec4501b1f18591ad0b9851
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Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype

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