Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

Single particle cryo-electron microscopy of membrane proteins is limited by their small size and difficulty to orient. Here the authors generate recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure dete...

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Autores principales:	Somnath Mukherjee, Satchal K. Erramilli, Mark Ammirati, Frances J. D. Alvarez, Kimberly F. Fennell, Michael D. Purdy, Blazej M. Skrobek, Katarzyna Radziwon, John Coukos, Yanyong Kang, Przemysław Dutka, Xiang Gao, Xiayang Qiu, Mark Yeager, H. Eric Xu, Seungil Han, Anthony A. Kossiakoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c4e601bd5c28456ba07689558d4667e5
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spelling	oai:doaj.org-article:c4e601bd5c28456ba07689558d4667e52021-12-02T17:31:11ZSynthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins10.1038/s41467-020-15363-02041-1723https://doaj.org/article/c4e601bd5c28456ba07689558d4667e52020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15363-0https://doaj.org/toc/2041-1723Single particle cryo-electron microscopy of membrane proteins is limited by their small size and difficulty to orient. Here the authors generate recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure determination of BRIL fused membrane proteins.Somnath MukherjeeSatchal K. ErramilliMark AmmiratiFrances J. D. AlvarezKimberly F. FennellMichael D. PurdyBlazej M. SkrobekKatarzyna RadziwonJohn CoukosYanyong KangPrzemysław DutkaXiang GaoXiayang QiuMark YeagerH. Eric XuSeungil HanAnthony A. KossiakoffNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Somnath Mukherjee Satchal K. Erramilli Mark Ammirati Frances J. D. Alvarez Kimberly F. Fennell Michael D. Purdy Blazej M. Skrobek Katarzyna Radziwon John Coukos Yanyong Kang Przemysław Dutka Xiang Gao Xiayang Qiu Mark Yeager H. Eric Xu Seungil Han Anthony A. Kossiakoff Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
description	Single particle cryo-electron microscopy of membrane proteins is limited by their small size and difficulty to orient. Here the authors generate recombinant antibodies against the 12 kDa fusion partner BRIL domain from apocytochrome b562 to use them as plug and play fiducial marks for structure determination of BRIL fused membrane proteins.
format	article
author	Somnath Mukherjee Satchal K. Erramilli Mark Ammirati Frances J. D. Alvarez Kimberly F. Fennell Michael D. Purdy Blazej M. Skrobek Katarzyna Radziwon John Coukos Yanyong Kang Przemysław Dutka Xiang Gao Xiayang Qiu Mark Yeager H. Eric Xu Seungil Han Anthony A. Kossiakoff
author_facet	Somnath Mukherjee Satchal K. Erramilli Mark Ammirati Frances J. D. Alvarez Kimberly F. Fennell Michael D. Purdy Blazej M. Skrobek Katarzyna Radziwon John Coukos Yanyong Kang Przemysław Dutka Xiang Gao Xiayang Qiu Mark Yeager H. Eric Xu Seungil Han Anthony A. Kossiakoff
author_sort	Somnath Mukherjee
title	Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
title_short	Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
title_full	Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
title_fullStr	Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
title_full_unstemmed	Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins
title_sort	synthetic antibodies against bril as universal fiducial marks for single−particle cryoem structure determination of membrane proteins
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c4e601bd5c28456ba07689558d4667e5
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Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

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