Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses

Abstract Water quality is critical for egg production and animal health in commercial layer housing systems. To investigate microbial contamination in nipple drinking system in layer houses, the bacterial abundance and communities in water pipes and V-troughs on different tiers (e.g., 1st, 3rd, 5th,...

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Autores principales:	Yi Wan, Ruiyu Ma, Lilong Chai, Qiang Du, Rongbin Yang, Renrong Qi, Wei Liu, Junying Li, Yan Li, Kai Zhan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4e6e46cd70d447d9b1301d031a71fdc
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spelling	oai:doaj.org-article:c4e6e46cd70d447d9b1301d031a71fdc2021-12-02T19:17:05ZDetermination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses10.1038/s41598-021-98330-z2045-2322https://doaj.org/article/c4e6e46cd70d447d9b1301d031a71fdc2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98330-zhttps://doaj.org/toc/2045-2322Abstract Water quality is critical for egg production and animal health in commercial layer housing systems. To investigate microbial contamination in nipple drinking system in layer houses, the bacterial abundance and communities in water pipes and V-troughs on different tiers (e.g., 1st, 3rd, 5th, and 7th tiers) of a layer house with 8 overlapping cage tiers were determined using qRT-PCR and 16S rRNA sequencing. The water bacterial abundance (i.e., genome 16S rDNA copy number, WBCN) in water pipes and V-troughs did not significantly differ among tiers, but they were 46.77 to 1905.46 times higher in V-troughs than that in water pipes (P < 0.05) for each tier. Illumina sequencing obtained 1,746,303 effective reads from 24 water samples in V-troughs of 4 tiers (six samples from each tier). Taxonomic analysis indicated that the 1st and 5th tiers were predominated by Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, while the 3rd and 7th tiers were predominated by Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. The top four genera were Acinetobacter, Streptococcus, Rothia and Comamonas among measured tiers. The high bacterial abundance and bacterial OTUs of water in the V-troughs reflect poor water quality, which may adversely affect growth and health of laying hens. Therefore, it is suggested that water quality in the V-tough should be checked more frequently in commercial layer houses.Yi WanRuiyu MaLilong ChaiQiang DuRongbin YangRenrong QiWei LiuJunying LiYan LiKai ZhanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Wan Ruiyu Ma Lilong Chai Qiang Du Rongbin Yang Renrong Qi Wei Liu Junying Li Yan Li Kai Zhan Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
description	Abstract Water quality is critical for egg production and animal health in commercial layer housing systems. To investigate microbial contamination in nipple drinking system in layer houses, the bacterial abundance and communities in water pipes and V-troughs on different tiers (e.g., 1st, 3rd, 5th, and 7th tiers) of a layer house with 8 overlapping cage tiers were determined using qRT-PCR and 16S rRNA sequencing. The water bacterial abundance (i.e., genome 16S rDNA copy number, WBCN) in water pipes and V-troughs did not significantly differ among tiers, but they were 46.77 to 1905.46 times higher in V-troughs than that in water pipes (P < 0.05) for each tier. Illumina sequencing obtained 1,746,303 effective reads from 24 water samples in V-troughs of 4 tiers (six samples from each tier). Taxonomic analysis indicated that the 1st and 5th tiers were predominated by Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, while the 3rd and 7th tiers were predominated by Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. The top four genera were Acinetobacter, Streptococcus, Rothia and Comamonas among measured tiers. The high bacterial abundance and bacterial OTUs of water in the V-troughs reflect poor water quality, which may adversely affect growth and health of laying hens. Therefore, it is suggested that water quality in the V-tough should be checked more frequently in commercial layer houses.
format	article
author	Yi Wan Ruiyu Ma Lilong Chai Qiang Du Rongbin Yang Renrong Qi Wei Liu Junying Li Yan Li Kai Zhan
author_facet	Yi Wan Ruiyu Ma Lilong Chai Qiang Du Rongbin Yang Renrong Qi Wei Liu Junying Li Yan Li Kai Zhan
author_sort	Yi Wan
title	Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
title_short	Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
title_full	Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
title_fullStr	Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
title_full_unstemmed	Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
title_sort	determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c4e6e46cd70d447d9b1301d031a71fdc
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Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses

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