Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface

Shanshan Ma,1,* Zibin Wang,2,* Yu Guo,1 Peng Wang,3 Zukun Yang,1 Liping Han,1 Jianfei Sun,4 Yang Xia1,4 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2Analysis and Test Center, Nanjing Medical University, Nanjing, Jiangsu 211166, Chin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ma S, Wang Z, Guo Y, Wang P, Yang Z, Han L, Sun J, Xia Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	layer-by-layer assembly osteogenesis nanotechnology bone tissue engineering interface. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c4e93e679ca64a19a05c77b3e5d23b1e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c4e93e679ca64a19a05c77b3e5d23b1e
record_format	dspace
spelling	oai:doaj.org-article:c4e93e679ca64a19a05c77b3e5d23b1e2021-12-02T03:59:23ZEnhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface1178-2013https://doaj.org/article/c4e93e679ca64a19a05c77b3e5d23b1e2019-02-01T00:00:00Zhttps://www.dovepress.com/enhanced-osteoinduction-of-electrospun-scaffolds-with-assemblies-of-he-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shanshan Ma,1,* Zibin Wang,2,* Yu Guo,1 Peng Wang,3 Zukun Yang,1 Liping Han,1 Jianfei Sun,4 Yang Xia1,4 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2Analysis and Test Center, Nanjing Medical University, Nanjing, Jiangsu 211166, China; 3Department of Sports Medicine and Adult Reconstructive Surgery, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, Jiangsu 210008, China; 4Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu 210096, China *These authors contributed equally to this work Purpose: Electrospun scaffolds have been studied extensively for their potential use in bone tissue engineering. However, their hydrophobicity and relatively low matrix stiffness constrain their osteoinduction capacities. In the present study, we studied polymer electrospun scaffolds coated with hydrophilic hematite nanoparticles (αFeNPs) constructed using layer-by-layer (LbL) assembly to construct a bioactive interface between the scaffolds and cells, to improve the osteoinduction capacities of the scaffolds.Materials and methods: The morphology of the αFeNPs was assessed. Surface properties of the scaffolds were tested by X-ray photoelectron spectroscopy (XPS), surface water contact angle, and in vitro protein adsorption test. The stiffness of the coating was tested using an atomic force microscope (AFM). In vitro cell assays were performed using rat adipose-derived stem cells (ADSCs).Results: Morphology characterizations showed that αFeNPs assembled on the surface of the scaffold, where the nano assemblies improved hydrophilicity and increased surface roughness, with increased surface stiffness. Enhanced initial ADSC cell spread was found in the nano assembled groups. Significant enhancements in osteogenic differentiation, represented by enhanced alkaline phosphatase (ALP) activities, elevated expression of osteogenic marker genes, and increased mineral synthesis by the seeded ADSCs, were detected. The influencing factors were attributed to the better hydrophilicity, rougher surface topography, and harder interface stiffness. In addition, the presence of nanoparticles was believed to provide better cell adhesion sites.Conclusion: The results suggested that the construction of a bioactive interface by LbL assembly using αFeNPs on traditional scaffolds should be a promising method for bone tissue engineering. Keywords: layer-by-layer assembly, osteogenesis, nanotechnology, bone tissue engineering, surface, αFeNPs  Ma SWang ZGuo YWang PYang ZHan LSun JXia YDove Medical Pressarticlelayer-by-layer assemblyosteogenesisnanotechnologybone tissue engineeringinterface.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1051-1068 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	layer-by-layer assembly osteogenesis nanotechnology bone tissue engineering interface. Medicine (General) R5-920
spellingShingle	layer-by-layer assembly osteogenesis nanotechnology bone tissue engineering interface. Medicine (General) R5-920 Ma S Wang Z Guo Y Wang P Yang Z Han L Sun J Xia Y Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
description	Shanshan Ma,1,* Zibin Wang,2,* Yu Guo,1 Peng Wang,3 Zukun Yang,1 Liping Han,1 Jianfei Sun,4 Yang Xia1,4 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China; 2Analysis and Test Center, Nanjing Medical University, Nanjing, Jiangsu 211166, China; 3Department of Sports Medicine and Adult Reconstructive Surgery, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, Jiangsu 210008, China; 4Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu 210096, China *These authors contributed equally to this work Purpose: Electrospun scaffolds have been studied extensively for their potential use in bone tissue engineering. However, their hydrophobicity and relatively low matrix stiffness constrain their osteoinduction capacities. In the present study, we studied polymer electrospun scaffolds coated with hydrophilic hematite nanoparticles (αFeNPs) constructed using layer-by-layer (LbL) assembly to construct a bioactive interface between the scaffolds and cells, to improve the osteoinduction capacities of the scaffolds.Materials and methods: The morphology of the αFeNPs was assessed. Surface properties of the scaffolds were tested by X-ray photoelectron spectroscopy (XPS), surface water contact angle, and in vitro protein adsorption test. The stiffness of the coating was tested using an atomic force microscope (AFM). In vitro cell assays were performed using rat adipose-derived stem cells (ADSCs).Results: Morphology characterizations showed that αFeNPs assembled on the surface of the scaffold, where the nano assemblies improved hydrophilicity and increased surface roughness, with increased surface stiffness. Enhanced initial ADSC cell spread was found in the nano assembled groups. Significant enhancements in osteogenic differentiation, represented by enhanced alkaline phosphatase (ALP) activities, elevated expression of osteogenic marker genes, and increased mineral synthesis by the seeded ADSCs, were detected. The influencing factors were attributed to the better hydrophilicity, rougher surface topography, and harder interface stiffness. In addition, the presence of nanoparticles was believed to provide better cell adhesion sites.Conclusion: The results suggested that the construction of a bioactive interface by LbL assembly using αFeNPs on traditional scaffolds should be a promising method for bone tissue engineering. Keywords: layer-by-layer assembly, osteogenesis, nanotechnology, bone tissue engineering, surface, αFeNPs
format	article
author	Ma S Wang Z Guo Y Wang P Yang Z Han L Sun J Xia Y
author_facet	Ma S Wang Z Guo Y Wang P Yang Z Han L Sun J Xia Y
author_sort	Ma S
title	Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
title_short	Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
title_full	Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
title_fullStr	Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
title_full_unstemmed	Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
title_sort	enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/c4e93e679ca64a19a05c77b3e5d23b1e
work_keys_str_mv	AT mas enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT wangz enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT guoy enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT wangp enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT yangz enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT hanl enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT sunj enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface AT xiay enhancedosteoinductionofelectrospunscaffoldswithassembliesofhematitenanoparticlesasabioactiveinterface
_version_	1718401515588157440

Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface

Ejemplares similares