Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats

BACKGROUND AND OBJECTIVE: Sour orange is known as an herbal plant in traditional medicine. Previous studies indicate a protective effect of citrus fruits or juices against risk of some chronic diseases such as diabetes. The aim of this study was to investigate the antioxidant and anti- hyperglycemic...

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Autores principales:	M Lakzaei, M Pouramir, AA Moghadamnia, H Mir
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	sour orange alloxan insulin hyperglycemia antioxidant Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c4f27e3c4dda42918e0932ddf0749c69
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spelling	oai:doaj.org-article:c4f27e3c4dda42918e0932ddf0749c692021-11-10T08:52:02ZEffect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats1561-41072251-7170https://doaj.org/article/c4f27e3c4dda42918e0932ddf0749c692012-01-01T00:00:00Zhttp://jbums.org/article-1-3978-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Sour orange is known as an herbal plant in traditional medicine. Previous studies indicate a protective effect of citrus fruits or juices against risk of some chronic diseases such as diabetes. The aim of this study was to investigate the antioxidant and anti- hyperglycemic effects of alcoholic extract of sour orange peel in rats with alloxan induced hyperglycemia.METHODS: In this experimental study, there were 120 Wistar rats weighing 150-200 gr that were divided into 6 groups (20 rats in each group) divided into two time groups (24 and 48 h). Ethanol extract of sour orange peel was prepared. Animals were pretreated for 4 days in 2 different doses 100 and 300 mg/kg. 100 mg/kg bw alloxan injected intraperitoneally. After 24, 48 h blood sample collected and glucose (via glucose kit), serum antioxidant levels (via FRAP) and serum insulin levels (via ELISA assay) were measured and the groups were compared.FINDINGS: Twenty four hours after alloxan injection rats who received 300 mg/kg/day showed a significant increase in total antioxidant content (p=0.000) and significant increase in glucose level after 48 hours (p=0.001). The administration of sour orange peel extract (100 and 300 mg/kg/day) decreased serum insulin level in alloxan-induced hyperglycemic rats at 24 and 48 hours after alloxan injection (p<0.03).CONCLUSION: This study demonstrated that treatment with sour orange peel extract not only brought a significant increase insulin concentration but also decreased the insulin level and also increased glucose concentration in alloxanized rats.M LakzaeiM PouramirAA MoghadamniaH MirBabol University of Medical Sciencesarticlesour orangealloxaninsulinhyperglycemiaantioxidantMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 1, Pp 19-24 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	sour orange alloxan insulin hyperglycemia antioxidant Medicine R Medicine (General) R5-920
spellingShingle	sour orange alloxan insulin hyperglycemia antioxidant Medicine R Medicine (General) R5-920 M Lakzaei M Pouramir AA Moghadamnia H Mir Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
description	BACKGROUND AND OBJECTIVE: Sour orange is known as an herbal plant in traditional medicine. Previous studies indicate a protective effect of citrus fruits or juices against risk of some chronic diseases such as diabetes. The aim of this study was to investigate the antioxidant and anti- hyperglycemic effects of alcoholic extract of sour orange peel in rats with alloxan induced hyperglycemia.METHODS: In this experimental study, there were 120 Wistar rats weighing 150-200 gr that were divided into 6 groups (20 rats in each group) divided into two time groups (24 and 48 h). Ethanol extract of sour orange peel was prepared. Animals were pretreated for 4 days in 2 different doses 100 and 300 mg/kg. 100 mg/kg bw alloxan injected intraperitoneally. After 24, 48 h blood sample collected and glucose (via glucose kit), serum antioxidant levels (via FRAP) and serum insulin levels (via ELISA assay) were measured and the groups were compared.FINDINGS: Twenty four hours after alloxan injection rats who received 300 mg/kg/day showed a significant increase in total antioxidant content (p=0.000) and significant increase in glucose level after 48 hours (p=0.001). The administration of sour orange peel extract (100 and 300 mg/kg/day) decreased serum insulin level in alloxan-induced hyperglycemic rats at 24 and 48 hours after alloxan injection (p<0.03).CONCLUSION: This study demonstrated that treatment with sour orange peel extract not only brought a significant increase insulin concentration but also decreased the insulin level and also increased glucose concentration in alloxanized rats.
format	article
author	M Lakzaei M Pouramir AA Moghadamnia H Mir
author_facet	M Lakzaei M Pouramir AA Moghadamnia H Mir
author_sort	M Lakzaei
title	Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
title_short	Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
title_full	Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
title_fullStr	Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
title_full_unstemmed	Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats
title_sort	effect of alcoholic extract of sour orange peel on total antioxidant, insulin level and hyperglycemia in alloxanized rats
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/c4f27e3c4dda42918e0932ddf0749c69
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Effect of Alcoholic Extract of Sour Orange Peel on Total Antioxidant, Insulin Level and Hyperglycemia in Alloxanized Rats

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