Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells

Abstract Due to their immunomodulatory and regenerative properties, Mesenchymal stromal cells (MSC) have generated major interests in several clinical settings including transplantation and inflammatory diseases. MSC functions can be influenced by their tissue origin. Their microenvironment strongly...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gordana Raicevic, Mehdi Najar, Hélène Busser, Emerence Crompot, Dominique Bron, Michel Toungouz, Laurence Lagneaux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4f45acc6d314b89b970f6886741dffd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c4f45acc6d314b89b970f6886741dffd
record_format	dspace
spelling	oai:doaj.org-article:c4f45acc6d314b89b970f6886741dffd2021-12-02T16:06:21ZComparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells10.1038/s41598-017-02850-62045-2322https://doaj.org/article/c4f45acc6d314b89b970f6886741dffd2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02850-6https://doaj.org/toc/2045-2322Abstract Due to their immunomodulatory and regenerative properties, Mesenchymal stromal cells (MSC) have generated major interests in several clinical settings including transplantation and inflammatory diseases. MSC functions can be influenced by their tissue origin. Their microenvironment strongly affects their biology notably through TLR sensing. In this study, we show that MSC isolated from four different sources express another type of cytosolic pathogen recognition receptors known as retinoic acid inducible gene-I (RIG-I)-like receptors (RLR). RLR activation in MSC induces the production of Type I IFN (IFN-β) and Type III IFN (IFN-λ1). The highest producers are adipose tissue(AT)-MSC. We further show that Interferon production is induced through TBK1/IKK-ε signaling and IRF7 phosphorylation. Depending on MSC source, the knockdown of TLR3 and/or RIG-I decreases the MSC response to RLR ligand poly(I:C)/Lyovec. Among the different MSC types, AT-MSCs display the highest sensitivity to viral stimuli as shown by the alteration of their viability after prolonged stimulation. Our work indicates that this could be linked to an increase of pro-apoptotic Noxa expression. Finally, the expression of IDO1 and LIF upon RLR activation indicate the increase of MSC immunomodulatory potential, especially in AT-MSCs. Altogether, these data should be considered when designing MSC-based therapy in clinical settings where inflammation or infection are present.Gordana RaicevicMehdi NajarHélène BusserEmerence CrompotDominique BronMichel ToungouzLaurence LagneauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gordana Raicevic Mehdi Najar Hélène Busser Emerence Crompot Dominique Bron Michel Toungouz Laurence Lagneaux Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
description	Abstract Due to their immunomodulatory and regenerative properties, Mesenchymal stromal cells (MSC) have generated major interests in several clinical settings including transplantation and inflammatory diseases. MSC functions can be influenced by their tissue origin. Their microenvironment strongly affects their biology notably through TLR sensing. In this study, we show that MSC isolated from four different sources express another type of cytosolic pathogen recognition receptors known as retinoic acid inducible gene-I (RIG-I)-like receptors (RLR). RLR activation in MSC induces the production of Type I IFN (IFN-β) and Type III IFN (IFN-λ1). The highest producers are adipose tissue(AT)-MSC. We further show that Interferon production is induced through TBK1/IKK-ε signaling and IRF7 phosphorylation. Depending on MSC source, the knockdown of TLR3 and/or RIG-I decreases the MSC response to RLR ligand poly(I:C)/Lyovec. Among the different MSC types, AT-MSCs display the highest sensitivity to viral stimuli as shown by the alteration of their viability after prolonged stimulation. Our work indicates that this could be linked to an increase of pro-apoptotic Noxa expression. Finally, the expression of IDO1 and LIF upon RLR activation indicate the increase of MSC immunomodulatory potential, especially in AT-MSCs. Altogether, these data should be considered when designing MSC-based therapy in clinical settings where inflammation or infection are present.
format	article
author	Gordana Raicevic Mehdi Najar Hélène Busser Emerence Crompot Dominique Bron Michel Toungouz Laurence Lagneaux
author_facet	Gordana Raicevic Mehdi Najar Hélène Busser Emerence Crompot Dominique Bron Michel Toungouz Laurence Lagneaux
author_sort	Gordana Raicevic
title	Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
title_short	Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
title_full	Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
title_fullStr	Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
title_full_unstemmed	Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells
title_sort	comparison and immunobiological characterization of retinoic acid inducible gene-i-like receptor expression in mesenchymal stromal cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c4f45acc6d314b89b970f6886741dffd
work_keys_str_mv	AT gordanaraicevic comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT mehdinajar comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT helenebusser comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT emerencecrompot comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT dominiquebron comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT micheltoungouz comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells AT laurencelagneaux comparisonandimmunobiologicalcharacterizationofretinoicacidinduciblegeneilikereceptorexpressioninmesenchymalstromalcells
_version_	1718385052687007744

Comparison and immunobiological characterization of retinoic acid inducible gene-I-like receptor expression in mesenchymal stromal cells

Ejemplares similares