Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.

Gap junction (GJ) channels have been recognized as an important mechanism for synchronizing neuronal networks. Herein, we investigated the participation of GJ channels in the pilocarpine-induced status epilepticus (SE) by analyzing electrophysiological activity following the blockade of connexins (C...

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Autores principales:	Erika R Kinjo, Guilherme S V Higa, Edgard Morya, Angela C Valle, Alexandre H Kihara, Luiz R G Britto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4f93c7886684e7794ee56279b96dc8a
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spelling	oai:doaj.org-article:c4f93c7886684e7794ee56279b96dc8a2021-11-25T05:57:17ZReciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.1932-620310.1371/journal.pone.0109149https://doaj.org/article/c4f93c7886684e7794ee56279b96dc8a2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109149https://doaj.org/toc/1932-6203Gap junction (GJ) channels have been recognized as an important mechanism for synchronizing neuronal networks. Herein, we investigated the participation of GJ channels in the pilocarpine-induced status epilepticus (SE) by analyzing electrophysiological activity following the blockade of connexins (Cx)-mediated communication. In addition, we examined the regulation of gene expression, protein levels, phosphorylation profile and distribution of neuronal Cx36, Cx45 and glial Cx43 in the rat hippocampus during the acute and latent periods. Electrophysiological recordings revealed that the GJ blockade anticipates the occurrence of low voltage oscillations and promotes a marked reduction of power in all analyzed frequencies.Cx36 gene expression and protein levels remained stable in acute and latent periods, whereas upregulation of Cx45 gene expression and protein redistribution were detected in the latent period. We also observed upregulation of Cx43 mRNA levels followed by changes in the phosphorylation profile and protein accumulation. Taken together, our results indisputably revealed that GJ communication participates in the epileptiform activity induced by pilocarpine. Moreover, considering that specific Cxs undergo alterations through acute and latent periods, this study indicates that the control of GJ communication may represent a focus in reliable anti-epileptogenic strategies.Erika R KinjoGuilherme S V HigaEdgard MoryaAngela C ValleAlexandre H KiharaLuiz R G BrittoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109149 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erika R Kinjo Guilherme S V Higa Edgard Morya Angela C Valle Alexandre H Kihara Luiz R G Britto Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
description	Gap junction (GJ) channels have been recognized as an important mechanism for synchronizing neuronal networks. Herein, we investigated the participation of GJ channels in the pilocarpine-induced status epilepticus (SE) by analyzing electrophysiological activity following the blockade of connexins (Cx)-mediated communication. In addition, we examined the regulation of gene expression, protein levels, phosphorylation profile and distribution of neuronal Cx36, Cx45 and glial Cx43 in the rat hippocampus during the acute and latent periods. Electrophysiological recordings revealed that the GJ blockade anticipates the occurrence of low voltage oscillations and promotes a marked reduction of power in all analyzed frequencies.Cx36 gene expression and protein levels remained stable in acute and latent periods, whereas upregulation of Cx45 gene expression and protein redistribution were detected in the latent period. We also observed upregulation of Cx43 mRNA levels followed by changes in the phosphorylation profile and protein accumulation. Taken together, our results indisputably revealed that GJ communication participates in the epileptiform activity induced by pilocarpine. Moreover, considering that specific Cxs undergo alterations through acute and latent periods, this study indicates that the control of GJ communication may represent a focus in reliable anti-epileptogenic strategies.
format	article
author	Erika R Kinjo Guilherme S V Higa Edgard Morya Angela C Valle Alexandre H Kihara Luiz R G Britto
author_facet	Erika R Kinjo Guilherme S V Higa Edgard Morya Angela C Valle Alexandre H Kihara Luiz R G Britto
author_sort	Erika R Kinjo
title	Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
title_short	Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
title_full	Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
title_fullStr	Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
title_full_unstemmed	Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
title_sort	reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c4f93c7886684e7794ee56279b96dc8a
work_keys_str_mv	AT erikarkinjo reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus AT guilhermesvhiga reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus AT edgardmorya reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus AT angelacvalle reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus AT alexandrehkihara reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus AT luizrgbritto reciprocalregulationofepileptiformneuronaloscillationsandelectricalsynapsesintherathippocampus
_version_	1718414366600069120

Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus.

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