Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors

Many studies have found that gold nanoparticles with branched surfaces (nanoflowers) are markers for immunosensors that provide higher sensitivity gains than the commonly used spherical gold nanoparticles. Although the analytical characteristics of nanoparticle-using systems vary significantly depen...

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Autores principales:	Nadezhda A. Taranova, Nadezhda A. Byzova, Svetlana M. Pridvorova, Anatoly V. Zherdev, Boris B. Dzantiev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoparticles nanoflowers antibodies immunochromatography test strips cardiac biomarkers Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/c4fbb24c76b646ca89de07525bc0794c
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spelling	oai:doaj.org-article:c4fbb24c76b646ca89de07525bc0794c2021-11-11T19:06:39ZComparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors10.3390/s212170981424-8220https://doaj.org/article/c4fbb24c76b646ca89de07525bc0794c2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/21/7098https://doaj.org/toc/1424-8220Many studies have found that gold nanoparticles with branched surfaces (nanoflowers) are markers for immunosensors that provide higher sensitivity gains than the commonly used spherical gold nanoparticles. Although the analytical characteristics of nanoparticle-using systems vary significantly depending on their size and shape, the question of choosing the best gold nanoflowers remains open. This work presents a comparative study of a panel of 33 preparations of gold nanoflowers formed by varying several parameters: the size of spherical nanoparticles-nuclei, the concentrations of nuclei, and tetrachloroauric acid during growth. The sizes of the resulting particles, their sorption capacity under antibody immobilization, mobility along membranes for lateral flow assays, and the effects of these parameters on the limits of detection of lateral flow immunoassay are characterized. The optimality of preparations obtained by growing a 0.2% <i>v</i>/<i>v</i> solution of nuclei with a diameter of 10 or 20 nm with tetrachloroauric acid at a concentration of 0.12 mM was shown. With their use, lateral flow immune tests were developed to determine markers of acute myocardial infarction—fatty acids binding protein and troponins I and T. The use of gold nanoflowers obtained under the proposed protocols led to significant gains in the limits of detection—3 to 10 times under visual detection and over 100 times under instrumental detection—compared to spherical gold nanoparticles. The significant increase under instrumental detection is due to the label’s low nonspecific binding.Nadezhda A. TaranovaNadezhda A. ByzovaSvetlana M. PridvorovaAnatoly V. ZherdevBoris B. DzantievMDPI AGarticlenanoparticlesnanoflowersantibodiesimmunochromatographytest stripscardiac biomarkersChemical technologyTP1-1185ENSensors, Vol 21, Iss 7098, p 7098 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles nanoflowers antibodies immunochromatography test strips cardiac biomarkers Chemical technology TP1-1185
spellingShingle	nanoparticles nanoflowers antibodies immunochromatography test strips cardiac biomarkers Chemical technology TP1-1185 Nadezhda A. Taranova Nadezhda A. Byzova Svetlana M. Pridvorova Anatoly V. Zherdev Boris B. Dzantiev Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
description	Many studies have found that gold nanoparticles with branched surfaces (nanoflowers) are markers for immunosensors that provide higher sensitivity gains than the commonly used spherical gold nanoparticles. Although the analytical characteristics of nanoparticle-using systems vary significantly depending on their size and shape, the question of choosing the best gold nanoflowers remains open. This work presents a comparative study of a panel of 33 preparations of gold nanoflowers formed by varying several parameters: the size of spherical nanoparticles-nuclei, the concentrations of nuclei, and tetrachloroauric acid during growth. The sizes of the resulting particles, their sorption capacity under antibody immobilization, mobility along membranes for lateral flow assays, and the effects of these parameters on the limits of detection of lateral flow immunoassay are characterized. The optimality of preparations obtained by growing a 0.2% <i>v</i>/<i>v</i> solution of nuclei with a diameter of 10 or 20 nm with tetrachloroauric acid at a concentration of 0.12 mM was shown. With their use, lateral flow immune tests were developed to determine markers of acute myocardial infarction—fatty acids binding protein and troponins I and T. The use of gold nanoflowers obtained under the proposed protocols led to significant gains in the limits of detection—3 to 10 times under visual detection and over 100 times under instrumental detection—compared to spherical gold nanoparticles. The significant increase under instrumental detection is due to the label’s low nonspecific binding.
format	article
author	Nadezhda A. Taranova Nadezhda A. Byzova Svetlana M. Pridvorova Anatoly V. Zherdev Boris B. Dzantiev
author_facet	Nadezhda A. Taranova Nadezhda A. Byzova Svetlana M. Pridvorova Anatoly V. Zherdev Boris B. Dzantiev
author_sort	Nadezhda A. Taranova
title	Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
title_short	Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
title_full	Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
title_fullStr	Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
title_full_unstemmed	Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors
title_sort	comparative assessment of different gold nanoflowers as labels for lateral flow immunosensors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c4fbb24c76b646ca89de07525bc0794c
work_keys_str_mv	AT nadezhdaataranova comparativeassessmentofdifferentgoldnanoflowersaslabelsforlateralflowimmunosensors AT nadezhdaabyzova comparativeassessmentofdifferentgoldnanoflowersaslabelsforlateralflowimmunosensors AT svetlanampridvorova comparativeassessmentofdifferentgoldnanoflowersaslabelsforlateralflowimmunosensors AT anatolyvzherdev comparativeassessmentofdifferentgoldnanoflowersaslabelsforlateralflowimmunosensors AT borisbdzantiev comparativeassessmentofdifferentgoldnanoflowersaslabelsforlateralflowimmunosensors
_version_	1718431610039173120

Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors

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