Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling

Abstract Sesbania cannabina, a multipurpose leguminous crop, is highly resistant to waterlogging stress. However, the scant genomic resources in the genus Sesbania have greatly hindered further exploration of the mechanisms underlying its waterlogging tolerance. Here, the genetic basis of flooding t...

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Autores principales:	Cheng-Gang Ren, Cun-Cui Kong, Kun Yan, Hua Zhang, Yong-Ming Luo, Zhi-Hong Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c50864fc5eb9443886760eb60e949476
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spelling	oai:doaj.org-article:c50864fc5eb9443886760eb60e9494762021-12-02T15:05:43ZElucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling10.1038/s41598-017-07740-52045-2322https://doaj.org/article/c50864fc5eb9443886760eb60e9494762017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07740-5https://doaj.org/toc/2045-2322Abstract Sesbania cannabina, a multipurpose leguminous crop, is highly resistant to waterlogging stress. However, the scant genomic resources in the genus Sesbania have greatly hindered further exploration of the mechanisms underlying its waterlogging tolerance. Here, the genetic basis of flooding tolerance in S. cannabina was examined by transcriptome-wide gene expression changes using RNA-Seq in seedlings exposed to short-term (3 h) and long-term (27 h) waterlogging. After de- novo assembly, 213990 unigenes were identified, of which 145162 (79.6%) were annotated. Gene Ontology and pathway enrichment analyses revealed that the glycolysis and fermentation pathways were stimulated to produce ATP under hypoxic stress conditions. Energy-consuming biosynthetic processes were dramatically repressed by short and long term waterlogging, while amino acid metabolism was greatly induced to maintain ATP levels. The expression pattern of 10 unigenes involved in phenylpropanoid biosynthesis, glycolysis, and amino acid metabolism revealed by qRT-PCR confirmed the RNA-Seq data. The present study is a large-scale assessment of genomic resources of Sesbania and provides guidelines for probing the molecular mechanisms underlying S. cannabina waterlogging tolerance.Cheng-Gang RenCun-Cui KongKun YanHua ZhangYong-Ming LuoZhi-Hong XieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng-Gang Ren Cun-Cui Kong Kun Yan Hua Zhang Yong-Ming Luo Zhi-Hong Xie Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
description	Abstract Sesbania cannabina, a multipurpose leguminous crop, is highly resistant to waterlogging stress. However, the scant genomic resources in the genus Sesbania have greatly hindered further exploration of the mechanisms underlying its waterlogging tolerance. Here, the genetic basis of flooding tolerance in S. cannabina was examined by transcriptome-wide gene expression changes using RNA-Seq in seedlings exposed to short-term (3 h) and long-term (27 h) waterlogging. After de- novo assembly, 213990 unigenes were identified, of which 145162 (79.6%) were annotated. Gene Ontology and pathway enrichment analyses revealed that the glycolysis and fermentation pathways were stimulated to produce ATP under hypoxic stress conditions. Energy-consuming biosynthetic processes were dramatically repressed by short and long term waterlogging, while amino acid metabolism was greatly induced to maintain ATP levels. The expression pattern of 10 unigenes involved in phenylpropanoid biosynthesis, glycolysis, and amino acid metabolism revealed by qRT-PCR confirmed the RNA-Seq data. The present study is a large-scale assessment of genomic resources of Sesbania and provides guidelines for probing the molecular mechanisms underlying S. cannabina waterlogging tolerance.
format	article
author	Cheng-Gang Ren Cun-Cui Kong Kun Yan Hua Zhang Yong-Ming Luo Zhi-Hong Xie
author_facet	Cheng-Gang Ren Cun-Cui Kong Kun Yan Hua Zhang Yong-Ming Luo Zhi-Hong Xie
author_sort	Cheng-Gang Ren
title	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
title_short	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
title_full	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
title_fullStr	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
title_full_unstemmed	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
title_sort	elucidation of the molecular responses to waterlogging in sesbania cannabina roots by transcriptome profiling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c50864fc5eb9443886760eb60e949476
work_keys_str_mv	AT chenggangren elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling AT cuncuikong elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling AT kunyan elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling AT huazhang elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling AT yongmingluo elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling AT zhihongxie elucidationofthemolecularresponsestowaterlogginginsesbaniacannabinarootsbytranscriptomeprofiling
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Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling

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