Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice

Qiangqiang Wang, Xiaoke Sun, Xin Huang, Jianmei Huang, Muhammad Waqqas Hasan, RuoFeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People&r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang Q, Sun X, Huang X, Huang J, Hasan MW, Yan R, Xu L, Song X, Li X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	haemonchus contortus rhca59 chitosan plga nanovaccines Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c50d583de29a46e39dec777277e7786e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c50d583de29a46e39dec777277e7786e
record_format	dspace
spelling	oai:doaj.org-article:c50d583de29a46e39dec777277e7786e2021-12-02T15:01:03ZNanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice1178-2013https://doaj.org/article/c50d583de29a46e39dec777277e7786e2021-05-01T00:00:00Zhttps://www.dovepress.com/nanoparticles-of-chitosanpolydl-lactide-co-glycolide-enhanced-the-immu-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Qiangqiang Wang, Xiaoke Sun, Xin Huang, Jianmei Huang, Muhammad Waqqas Hasan, RuoFeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People’s Republic of ChinaCorrespondence: Xiangrui LiMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu Province, People’s Republic of ChinaTel/Fax +86 25 84399000Email lixiangrui@njau.edu.cnBackground: Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of Haemonchus contortus is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood.Purpose: The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of Haemonchus contortus HCA59 in mice.Methods: Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund’s complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry.Results: On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4+ T cells (CD3+ CD4+), CD8+ T cells (CD3+ CD8+), and dendritic cells (CD11c+ CD83+, CD11c+ CD86+) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group.Conclusion: The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against Haemonchus contortus in future study.Keywords: Haemonchus contortus, rHCA59, Chitosan, PLGA, nanovaccinesWang QSun XHuang XHuang JHasan MWYan RXu LSong XLi XDove Medical Pressarticlehaemonchus contortusrhca59chitosanplgananovaccinesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3125-3139 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	haemonchus contortus rhca59 chitosan plga nanovaccines Medicine (General) R5-920
spellingShingle	haemonchus contortus rhca59 chitosan plga nanovaccines Medicine (General) R5-920 Wang Q Sun X Huang X Huang J Hasan MW Yan R Xu L Song X Li X Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
description	Qiangqiang Wang, Xiaoke Sun, Xin Huang, Jianmei Huang, Muhammad Waqqas Hasan, RuoFeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People’s Republic of ChinaCorrespondence: Xiangrui LiMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu Province, People’s Republic of ChinaTel/Fax +86 25 84399000Email lixiangrui@njau.edu.cnBackground: Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of Haemonchus contortus is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood.Purpose: The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of Haemonchus contortus HCA59 in mice.Methods: Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund’s complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry.Results: On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4+ T cells (CD3+ CD4+), CD8+ T cells (CD3+ CD8+), and dendritic cells (CD11c+ CD83+, CD11c+ CD86+) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group.Conclusion: The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against Haemonchus contortus in future study.Keywords: Haemonchus contortus, rHCA59, Chitosan, PLGA, nanovaccines
format	article
author	Wang Q Sun X Huang X Huang J Hasan MW Yan R Xu L Song X Li X
author_facet	Wang Q Sun X Huang X Huang J Hasan MW Yan R Xu L Song X Li X
author_sort	Wang Q
title	Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
title_short	Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
title_full	Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
title_fullStr	Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
title_full_unstemmed	Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
title_sort	nanoparticles of chitosan/poly(d,l-lactide-co-glycolide) enhanced the immune responses of haemonchus contortus hca59 antigen in model mice
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c50d583de29a46e39dec777277e7786e
work_keys_str_mv	AT wangq nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT sunx nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT huangx nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT huangj nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT hasanmw nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT yanr nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT xul nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT songx nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice AT lix nanoparticlesofchitosanpolydllactidecoglycolideenhancedtheimmuneresponsesofhaemonchuscontortushca59antigeninmodelmice
_version_	1718389163524358144

Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice

Ejemplares similares