Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station

ABSTRACT Two isolates of Fusarium oxysporum, ISS-F3 and ISS-F4, were cultured from the dining table on the International Space Station (ISS). Genomic analyses using EF-1α sequences, presence/absence of effector proteins, k-mer comparisons, and single nucleotide polymorphisms indicate that these two...

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Autores principales:	Camilla Urbaniak, Peter van Dam, Alexander Zaborin, Olga Zaborina, Jack A. Gilbert, Tamas Torok, Clay C. C. Wang, Kasthuri Venkateswaran
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Fusarium International Space Station fungi genomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c50eb746343e4b93bfd6d95226d69402
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spelling	oai:doaj.org-article:c50eb746343e4b93bfd6d95226d694022021-12-02T18:39:16ZGenomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station10.1128/mSystems.00345-182379-5077https://doaj.org/article/c50eb746343e4b93bfd6d95226d694022019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00345-18https://doaj.org/toc/2379-5077ABSTRACT Two isolates of Fusarium oxysporum, ISS-F3 and ISS-F4, were cultured from the dining table on the International Space Station (ISS). Genomic analyses using EF-1α sequences, presence/absence of effector proteins, k-mer comparisons, and single nucleotide polymorphisms indicate that these two strains are genomically different from 65 known sequenced strains. Functional analysis revealed that ISS-F3/F4 had higher relative abundances of polyketide synthase domains than a non-plant-pathogenic soil isolate, used for biocontrol properties (Fo47), and a clinical isolate (FOSC-3a). Putative secondary metabolite analysis indicates that ISS-F3/F4 may produce yet-unreported polyketides and nonribosomal peptides. While genomic analysis showed that these ISS strains are unlikely to be plant pathogens, a virulence assay using an immunocompromised Caenorhabditis elegans model of fusariosis revealed that they were virulent and may represent opportunistic pathogens in animals, including humans. However, its effects on the health of immunocompromised humans warrant further study. IMPORTANCE This is the first study to isolate and characterize F. oxysporum isolates from a built environment, as well as one that has been exposed to space. The characterization and analysis of these two genomes may have important implications for the medical, agricultural, and food industries as well as for the health of the crew who coinhabit the ISS with these strains.Camilla UrbaniakPeter van DamAlexander ZaborinOlga ZaborinaJack A. GilbertTamas TorokClay C. C. WangKasthuri VenkateswaranAmerican Society for MicrobiologyarticleFusariumInternational Space StationfungigenomicsMicrobiologyQR1-502ENmSystems, Vol 4, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fusarium International Space Station fungi genomics Microbiology QR1-502
spellingShingle	Fusarium International Space Station fungi genomics Microbiology QR1-502 Camilla Urbaniak Peter van Dam Alexander Zaborin Olga Zaborina Jack A. Gilbert Tamas Torok Clay C. C. Wang Kasthuri Venkateswaran Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
description	ABSTRACT Two isolates of Fusarium oxysporum, ISS-F3 and ISS-F4, were cultured from the dining table on the International Space Station (ISS). Genomic analyses using EF-1α sequences, presence/absence of effector proteins, k-mer comparisons, and single nucleotide polymorphisms indicate that these two strains are genomically different from 65 known sequenced strains. Functional analysis revealed that ISS-F3/F4 had higher relative abundances of polyketide synthase domains than a non-plant-pathogenic soil isolate, used for biocontrol properties (Fo47), and a clinical isolate (FOSC-3a). Putative secondary metabolite analysis indicates that ISS-F3/F4 may produce yet-unreported polyketides and nonribosomal peptides. While genomic analysis showed that these ISS strains are unlikely to be plant pathogens, a virulence assay using an immunocompromised Caenorhabditis elegans model of fusariosis revealed that they were virulent and may represent opportunistic pathogens in animals, including humans. However, its effects on the health of immunocompromised humans warrant further study. IMPORTANCE This is the first study to isolate and characterize F. oxysporum isolates from a built environment, as well as one that has been exposed to space. The characterization and analysis of these two genomes may have important implications for the medical, agricultural, and food industries as well as for the health of the crew who coinhabit the ISS with these strains.
format	article
author	Camilla Urbaniak Peter van Dam Alexander Zaborin Olga Zaborina Jack A. Gilbert Tamas Torok Clay C. C. Wang Kasthuri Venkateswaran
author_facet	Camilla Urbaniak Peter van Dam Alexander Zaborin Olga Zaborina Jack A. Gilbert Tamas Torok Clay C. C. Wang Kasthuri Venkateswaran
author_sort	Camilla Urbaniak
title	Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
title_short	Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
title_full	Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
title_fullStr	Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
title_full_unstemmed	Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station
title_sort	genomic characterization and virulence potential of two <italic toggle="yes">fusarium oxysporum</italic> isolates cultured from the international space station
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/c50eb746343e4b93bfd6d95226d69402
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Genomic Characterization and Virulence Potential of Two <italic toggle="yes">Fusarium oxysporum</italic> Isolates Cultured from the International Space Station

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