Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice

Abstract Background Several studies have shown that probiotics have beneficial effects on weight control and metabolic health. In addition to probiotics, recent studies have investigated the effects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive effects of live and pasteur...

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Autores principales:	Fatemeh Ashrafian, Shahrbanoo Keshavarz Azizi Raftar, Arezou Lari, Arefeh Shahryari, Sara Abdollahiyan, Hamid Reza Moradi, Morteza Masoumi, Mehdi Davari, Shohreh khatami, Mir Davood Omrani, Farzam Vaziri, Andrea Masotti, Seyed Davar Siadat
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Akkermansia muciniphila Extracellular vesicles Obesity Pasteurization Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c51139714d5e48c188446dfa4a3dd47d
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spelling	oai:doaj.org-article:c51139714d5e48c188446dfa4a3dd47d2021-12-05T12:24:49ZExtracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice10.1186/s12934-021-01709-w1475-2859https://doaj.org/article/c51139714d5e48c188446dfa4a3dd47d2021-12-01T00:00:00Zhttps://doi.org/10.1186/s12934-021-01709-whttps://doaj.org/toc/1475-2859Abstract Background Several studies have shown that probiotics have beneficial effects on weight control and metabolic health. In addition to probiotics, recent studies have investigated the effects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive effects of live and pasteurized Akkermansia muciniphila MucT (A. muciniphila) and its extracellular vesicles (EVs) on HFD-induced obesity. Results The results showed that body weight, metabolic tissues weight, food consumption, and plasma metabolic parameters were increased in the HFD group, whereas A. muciniphila preventive treatments inhibited these HFD. The effects of pasteurized A. muciniphila and its extracellular vesicles were more noticeable than its active form. The HFD led to an increase in the colonic, adipose tissue, and liver inflammations and increased the expression of genes involved in lipid metabolism and homeostasis. Nevertheless, these effects were inhibited in mice that were administered A. muciniphila and its EVs. The assessment of the gut microbiota revealed significant differences in the microbiota composition after feeding with HFD. However, all treatments restored the alterations in some bacterial genera and closely resemble the control group. Also, the correlation analysis indicated that some gut microbiota might be associated with obesity-related indices. Conclusions Pasteurized A. muciniphila and its EVs, as paraprobiotic and postbiotic agents, were found to play a key role in the regulation of metabolic functions to prevent obesity, probably by affecting the gut-adipose-liver axis. Graphical AbstractFatemeh AshrafianShahrbanoo Keshavarz Azizi RaftarArezou LariArefeh ShahryariSara AbdollahiyanHamid Reza MoradiMorteza MasoumiMehdi DavariShohreh khatamiMir Davood OmraniFarzam VaziriAndrea MasottiSeyed Davar SiadatBMCarticleAkkermansia muciniphilaExtracellular vesiclesObesityPasteurizationMicrobiologyQR1-502ENMicrobial Cell Factories, Vol 20, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Akkermansia muciniphila Extracellular vesicles Obesity Pasteurization Microbiology QR1-502
spellingShingle	Akkermansia muciniphila Extracellular vesicles Obesity Pasteurization Microbiology QR1-502 Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Arefeh Shahryari Sara Abdollahiyan Hamid Reza Moradi Morteza Masoumi Mehdi Davari Shohreh khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
description	Abstract Background Several studies have shown that probiotics have beneficial effects on weight control and metabolic health. In addition to probiotics, recent studies have investigated the effects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive effects of live and pasteurized Akkermansia muciniphila MucT (A. muciniphila) and its extracellular vesicles (EVs) on HFD-induced obesity. Results The results showed that body weight, metabolic tissues weight, food consumption, and plasma metabolic parameters were increased in the HFD group, whereas A. muciniphila preventive treatments inhibited these HFD. The effects of pasteurized A. muciniphila and its extracellular vesicles were more noticeable than its active form. The HFD led to an increase in the colonic, adipose tissue, and liver inflammations and increased the expression of genes involved in lipid metabolism and homeostasis. Nevertheless, these effects were inhibited in mice that were administered A. muciniphila and its EVs. The assessment of the gut microbiota revealed significant differences in the microbiota composition after feeding with HFD. However, all treatments restored the alterations in some bacterial genera and closely resemble the control group. Also, the correlation analysis indicated that some gut microbiota might be associated with obesity-related indices. Conclusions Pasteurized A. muciniphila and its EVs, as paraprobiotic and postbiotic agents, were found to play a key role in the regulation of metabolic functions to prevent obesity, probably by affecting the gut-adipose-liver axis. Graphical Abstract
format	article
author	Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Arefeh Shahryari Sara Abdollahiyan Hamid Reza Moradi Morteza Masoumi Mehdi Davari Shohreh khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat
author_facet	Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Arefeh Shahryari Sara Abdollahiyan Hamid Reza Moradi Morteza Masoumi Mehdi Davari Shohreh khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat
author_sort	Fatemeh Ashrafian
title	Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
title_short	Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
title_full	Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
title_fullStr	Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
title_full_unstemmed	Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice
title_sort	extracellular vesicles and pasteurized cells derived from akkermansia muciniphila protect against high-fat induced obesity in mice
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c51139714d5e48c188446dfa4a3dd47d
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Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice

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