Spatially patterned excitatory neuron subtypes and projections of the claustrum

The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can sugge...

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Autores principales:	Sarah R Erwin, Brianna N Bristow, Kaitlin E Sullivan, Rennie M Kendrick, Brian Marriott, Lihua Wang, Jody Clements, Andrew L Lemire, Jesse Jackson, Mark S Cembrowski
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Claustrum transcriptome cell type Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c5118d08bf64455d8181eaea3b55aa16
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id	oai:doaj.org-article:c5118d08bf64455d8181eaea3b55aa16
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spelling	oai:doaj.org-article:c5118d08bf64455d8181eaea3b55aa162021-11-26T07:07:34ZSpatially patterned excitatory neuron subtypes and projections of the claustrum10.7554/eLife.689672050-084Xe68967https://doaj.org/article/c5118d08bf64455d8181eaea3b55aa162021-08-01T00:00:00Zhttps://elifesciences.org/articles/68967https://doaj.org/toc/2050-084XThe claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.Sarah R ErwinBrianna N BristowKaitlin E SullivanRennie M KendrickBrian MarriottLihua WangJody ClementsAndrew L LemireJesse JacksonMark S CembrowskieLife Sciences Publications LtdarticleClaustrumtranscriptomecell typeMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Claustrum transcriptome cell type Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Claustrum transcriptome cell type Medicine R Science Q Biology (General) QH301-705.5 Sarah R Erwin Brianna N Bristow Kaitlin E Sullivan Rennie M Kendrick Brian Marriott Lihua Wang Jody Clements Andrew L Lemire Jesse Jackson Mark S Cembrowski Spatially patterned excitatory neuron subtypes and projections of the claustrum
description	The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.
format	article
author	Sarah R Erwin Brianna N Bristow Kaitlin E Sullivan Rennie M Kendrick Brian Marriott Lihua Wang Jody Clements Andrew L Lemire Jesse Jackson Mark S Cembrowski
author_facet	Sarah R Erwin Brianna N Bristow Kaitlin E Sullivan Rennie M Kendrick Brian Marriott Lihua Wang Jody Clements Andrew L Lemire Jesse Jackson Mark S Cembrowski
author_sort	Sarah R Erwin
title	Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_short	Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_full	Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_fullStr	Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_full_unstemmed	Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_sort	spatially patterned excitatory neuron subtypes and projections of the claustrum
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/c5118d08bf64455d8181eaea3b55aa16
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Spatially patterned excitatory neuron subtypes and projections of the claustrum

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