Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>

<i>Streptococcus mutans</i> releases membrane vesicles (MVs) and induces MV-dependent biofilm formation. Glucosyltransferases (Gtfs) are bound to MVs and contribute to the adhesion and glucans-dependent biofilm formation of early adherent bacteria on the tooth surface. The biofilm format...

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Autores principales:	Yusuke Iwabuchi, Tomoyo Nakamura, Yasuka Kusumoto, Ryoma Nakao, Tsutomu Iwamoto, Osamu Shinozuka, Hidenobu Senpuku
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biofilm membrane vesicle GtfC initial pH acetic acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c513a7a1a10940a4a812a251db67f873
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spelling	oai:doaj.org-article:c513a7a1a10940a4a812a251db67f8732021-11-25T18:25:00ZEffects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>10.3390/microorganisms91123082076-2607https://doaj.org/article/c513a7a1a10940a4a812a251db67f8732021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2308https://doaj.org/toc/2076-2607<i>Streptococcus mutans</i> releases membrane vesicles (MVs) and induces MV-dependent biofilm formation. Glucosyltransferases (Gtfs) are bound to MVs and contribute to the adhesion and glucans-dependent biofilm formation of early adherent bacteria on the tooth surface. The biofilm formation of <i>S. mutans</i> may be controlled depending on whether the initial pH tends to be acidic or alkaline. In this study, the characteristics and effects of MVs extracted from various conditions {(initial pH 6.0 and 8.0 media prepared with lactic acid (LA) and acetic acid (AA), and with NaOH (NO), respectively)} on the biofilm formation of <i>S. mutans</i> and early adherent bacteria were investigated. The quantitative changes in glucans between primary pH 6.0 and 8.0 conditions were observed, associated with different activities affecting MV-dependent biofilm formation. The decreased amount of Gtfs on MVs under the initial pH 6.0 conditions strongly guided low levels of MV-dependent biofilm formation. However, in the initial pH 6.0 and 8.0 solutions prepared with AA and NO, the MVs in the biofilm appeared to be formed by the expression of glucans and/or extracellular DNA. These results suggest that the environmental pH conditions established by acid and alkaline factors determine the differences in the local pathogenic activities of biofilm development in the oral cavity.Yusuke IwabuchiTomoyo NakamuraYasuka KusumotoRyoma NakaoTsutomu IwamotoOsamu ShinozukaHidenobu SenpukuMDPI AGarticlebiofilmmembrane vesicleGtfCinitial pHacetic acidBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2308, p 2308 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm membrane vesicle GtfC initial pH acetic acid Biology (General) QH301-705.5
spellingShingle	biofilm membrane vesicle GtfC initial pH acetic acid Biology (General) QH301-705.5 Yusuke Iwabuchi Tomoyo Nakamura Yasuka Kusumoto Ryoma Nakao Tsutomu Iwamoto Osamu Shinozuka Hidenobu Senpuku Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
description	<i>Streptococcus mutans</i> releases membrane vesicles (MVs) and induces MV-dependent biofilm formation. Glucosyltransferases (Gtfs) are bound to MVs and contribute to the adhesion and glucans-dependent biofilm formation of early adherent bacteria on the tooth surface. The biofilm formation of <i>S. mutans</i> may be controlled depending on whether the initial pH tends to be acidic or alkaline. In this study, the characteristics and effects of MVs extracted from various conditions {(initial pH 6.0 and 8.0 media prepared with lactic acid (LA) and acetic acid (AA), and with NaOH (NO), respectively)} on the biofilm formation of <i>S. mutans</i> and early adherent bacteria were investigated. The quantitative changes in glucans between primary pH 6.0 and 8.0 conditions were observed, associated with different activities affecting MV-dependent biofilm formation. The decreased amount of Gtfs on MVs under the initial pH 6.0 conditions strongly guided low levels of MV-dependent biofilm formation. However, in the initial pH 6.0 and 8.0 solutions prepared with AA and NO, the MVs in the biofilm appeared to be formed by the expression of glucans and/or extracellular DNA. These results suggest that the environmental pH conditions established by acid and alkaline factors determine the differences in the local pathogenic activities of biofilm development in the oral cavity.
format	article
author	Yusuke Iwabuchi Tomoyo Nakamura Yasuka Kusumoto Ryoma Nakao Tsutomu Iwamoto Osamu Shinozuka Hidenobu Senpuku
author_facet	Yusuke Iwabuchi Tomoyo Nakamura Yasuka Kusumoto Ryoma Nakao Tsutomu Iwamoto Osamu Shinozuka Hidenobu Senpuku
author_sort	Yusuke Iwabuchi
title	Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
title_short	Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
title_full	Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
title_fullStr	Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
title_full_unstemmed	Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>
title_sort	effects of ph on the properties of membrane vesicles including glucosyltransferase in <i>streptococcus mutans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c513a7a1a10940a4a812a251db67f873
work_keys_str_mv	AT yusukeiwabuchi effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT tomoyonakamura effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT yasukakusumoto effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT ryomanakao effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT tsutomuiwamoto effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT osamushinozuka effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi AT hidenobusenpuku effectsofphonthepropertiesofmembranevesiclesincludingglucosyltransferaseinistreptococcusmutansi
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Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase in <i>Streptococcus mutans</i>

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