The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells

Background: Staurosporine-dependent single and collective cell migration patterns of breast carcinoma cells MDA-MB-231, MCF-7, and SK-BR-3 were analysed to characterise the presence of drug-dependent migration promoting and inhibiting yin-yang effects. Methods: Migration patterns of various breast c...

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Autores principales:	Frank A. H. Meyer, Dominik Kraus, Alexander Glassmann, Nadine Veit, Jochen Winter, Rainer Probstmeier
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell migration breast carcinoma invasion staurosporine yin-yang effect Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c514d252f7db4ca0820d558d422662cd
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spelling	oai:doaj.org-article:c514d252f7db4ca0820d558d422662cd2021-11-11T17:22:52ZThe Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells10.3390/ijms2221119611422-00671661-6596https://doaj.org/article/c514d252f7db4ca0820d558d422662cd2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11961https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Background: Staurosporine-dependent single and collective cell migration patterns of breast carcinoma cells MDA-MB-231, MCF-7, and SK-BR-3 were analysed to characterise the presence of drug-dependent migration promoting and inhibiting yin-yang effects. Methods: Migration patterns of various breast cancer cells after staurosporine treatment were investigated using Western blot, cell toxicity assays, single and collective cell migration assays, and video time-lapse. Statistical analyses were performed with Kruskal–Wallis and Fligner–Killeen tests. Results: Application of staurosporine induced the migration of single MCF-7 cells but inhibited collective cell migration. With the exception of low-density SK-BR-3 cells, staurosporine induced the generation of immobile flattened giant cells. Video time-lapse analysis revealed that within the borderline of cell collectives, staurosporine reduced the velocity of individual MDA-MB-231 and SK-BR-3, but not of MCF-7 cells. In individual MCF-7 cells, mainly the directionality of migration became disturbed, which led to an increased migration rate parallel to the borderline, and hereby to an inhibition of the migration of the cell collective as a total. Moreover, the application of staurosporine led to a transient activation of ERK1/2 in all cell lines. Conclusion: Dependent on the context (single versus collective cells), a drug may induce opposite effects in the same cell line.Frank A. H. MeyerDominik KrausAlexander GlassmannNadine VeitJochen WinterRainer ProbstmeierMDPI AGarticlecell migrationbreast carcinomainvasionstaurosporineyin-yang effectBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11961, p 11961 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell migration breast carcinoma invasion staurosporine yin-yang effect Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cell migration breast carcinoma invasion staurosporine yin-yang effect Biology (General) QH301-705.5 Chemistry QD1-999 Frank A. H. Meyer Dominik Kraus Alexander Glassmann Nadine Veit Jochen Winter Rainer Probstmeier The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
description	Background: Staurosporine-dependent single and collective cell migration patterns of breast carcinoma cells MDA-MB-231, MCF-7, and SK-BR-3 were analysed to characterise the presence of drug-dependent migration promoting and inhibiting yin-yang effects. Methods: Migration patterns of various breast cancer cells after staurosporine treatment were investigated using Western blot, cell toxicity assays, single and collective cell migration assays, and video time-lapse. Statistical analyses were performed with Kruskal–Wallis and Fligner–Killeen tests. Results: Application of staurosporine induced the migration of single MCF-7 cells but inhibited collective cell migration. With the exception of low-density SK-BR-3 cells, staurosporine induced the generation of immobile flattened giant cells. Video time-lapse analysis revealed that within the borderline of cell collectives, staurosporine reduced the velocity of individual MDA-MB-231 and SK-BR-3, but not of MCF-7 cells. In individual MCF-7 cells, mainly the directionality of migration became disturbed, which led to an increased migration rate parallel to the borderline, and hereby to an inhibition of the migration of the cell collective as a total. Moreover, the application of staurosporine led to a transient activation of ERK1/2 in all cell lines. Conclusion: Dependent on the context (single versus collective cells), a drug may induce opposite effects in the same cell line.
format	article
author	Frank A. H. Meyer Dominik Kraus Alexander Glassmann Nadine Veit Jochen Winter Rainer Probstmeier
author_facet	Frank A. H. Meyer Dominik Kraus Alexander Glassmann Nadine Veit Jochen Winter Rainer Probstmeier
author_sort	Frank A. H. Meyer
title	The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
title_short	The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
title_full	The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
title_fullStr	The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
title_full_unstemmed	The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells
title_sort	presence of yin-yang effects in the migration pattern of staurosporine-treated single versus collective breast carcinoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c514d252f7db4ca0820d558d422662cd
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The Presence of Yin-Yang Effects in the Migration Pattern of Staurosporine-Treated Single versus Collective Breast Carcinoma Cells

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