Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study

Background: Background: Botulinum toxin A (BtxA) is a powerful neurotoxin reported to be effective as a cancer adjuvant therapy with fewer side effects. Previously we showed the apoptotic effects of BtxA on the GBM cell line (U87-MG). In order to confirm the positive neurotoxicity of BtxA on other c...

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Autores principales:	Mona Farhadi, Melikasadat Jameie, Bahareh Derakhshanmehr, Moghadam Tahmasebi, Maryam Soleimani, Seyed Behnamedin Jameie
Formato:	article
Lenguaje:	EN
Publicado:	Shahid Beheshti University of Medical Sciences 2020
Materias:	btxa neurotoxicity apoptosis mcf-7 pc-3 sk-ov3 cho cell lines Medicine R
Acceso en línea:	https://doaj.org/article/c516698f4799467abaf57aa46e18af28
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spelling	oai:doaj.org-article:c516698f4799467abaf57aa46e18af282021-11-16T11:06:27ZNeurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study2383-18712383-209610.34172/icnj.2020.24https://doaj.org/article/c516698f4799467abaf57aa46e18af282020-10-01T00:00:00Zhttps://journals.sbmu.ac.ir/neuroscience/article/view/32419/25813https://doaj.org/toc/2383-1871https://doaj.org/toc/2383-2096Background: Background: Botulinum toxin A (BtxA) is a powerful neurotoxin reported to be effective as a cancer adjuvant therapy with fewer side effects. Previously we showed the apoptotic effects of BtxA on the GBM cell line (U87-MG). In order to confirm the positive neurotoxicity of BtxA on other cancer cell lines, including SK-OV-3, CHO, MCF-7, and PC-3, the present research has designed. Methods: The cell lines prepared, cultured, and exposed to different concentrations of BtxA for 24 and 48 hours. Using MTT, Annexin V/PI assays and western blotting, cell viability, and apoptosis studied. Results: Our results showed that different BtxA Botox concentrations led to significant cell death in each cell line in a dose-dependent manner (P<0.001) but did not for PC-3 cells. The results of the Annexin V/PI staining indicated that BtxA induced apoptosis after 24 hours. The 1.45U, 1.75U, and 1.65U of BtxA respectively induced more than 50% apoptosis in MCF 7, SK-OV-3, and CHO cell lines. The results of caspase 3 showed more protein expression in the treated group compares to the control group. Conclusion: BtxA, as a neurotoxin, can insert therapeutic anti-cancer and apoptotic effects on various types of cancerous cells; further studies need to illuminate the possible mechanisms.Mona FarhadiMelikasadat JameieBahareh DerakhshanmehrMoghadam TahmasebiMaryam SoleimaniSeyed Behnamedin JameieShahid Beheshti University of Medical Sciencesarticlebtxa neurotoxicityapoptosismcf-7pc-3sk-ov3cho cell linesMedicineRENInternational Clinical Neuroscience Journal, Vol 7, Iss 4, Pp 190-195 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	btxa neurotoxicity apoptosis mcf-7 pc-3 sk-ov3 cho cell lines Medicine R
spellingShingle	btxa neurotoxicity apoptosis mcf-7 pc-3 sk-ov3 cho cell lines Medicine R Mona Farhadi Melikasadat Jameie Bahareh Derakhshanmehr Moghadam Tahmasebi Maryam Soleimani Seyed Behnamedin Jameie Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
description	Background: Background: Botulinum toxin A (BtxA) is a powerful neurotoxin reported to be effective as a cancer adjuvant therapy with fewer side effects. Previously we showed the apoptotic effects of BtxA on the GBM cell line (U87-MG). In order to confirm the positive neurotoxicity of BtxA on other cancer cell lines, including SK-OV-3, CHO, MCF-7, and PC-3, the present research has designed. Methods: The cell lines prepared, cultured, and exposed to different concentrations of BtxA for 24 and 48 hours. Using MTT, Annexin V/PI assays and western blotting, cell viability, and apoptosis studied. Results: Our results showed that different BtxA Botox concentrations led to significant cell death in each cell line in a dose-dependent manner (P<0.001) but did not for PC-3 cells. The results of the Annexin V/PI staining indicated that BtxA induced apoptosis after 24 hours. The 1.45U, 1.75U, and 1.65U of BtxA respectively induced more than 50% apoptosis in MCF 7, SK-OV-3, and CHO cell lines. The results of caspase 3 showed more protein expression in the treated group compares to the control group. Conclusion: BtxA, as a neurotoxin, can insert therapeutic anti-cancer and apoptotic effects on various types of cancerous cells; further studies need to illuminate the possible mechanisms.
format	article
author	Mona Farhadi Melikasadat Jameie Bahareh Derakhshanmehr Moghadam Tahmasebi Maryam Soleimani Seyed Behnamedin Jameie
author_facet	Mona Farhadi Melikasadat Jameie Bahareh Derakhshanmehr Moghadam Tahmasebi Maryam Soleimani Seyed Behnamedin Jameie
author_sort	Mona Farhadi
title	Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
title_short	Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
title_full	Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
title_fullStr	Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
title_full_unstemmed	Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study
title_sort	neurotoxin botulinum inserts apoptotic effects on certain cancer cell lines via neural niche of tumors: a molecular study
publisher	Shahid Beheshti University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/c516698f4799467abaf57aa46e18af28
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Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study

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