Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography

Streptococcus mutans (Gram-positive) and Shigella dysenteriae (Gram-negative) are two types of pathogen bacteria. The use of synthetic antibiotics against both bacteria is known to impact the bacteria's resistance. E. spinosum from Tasikmalaya is a potential macroalgae as a source of an antibac...

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Autores principales:	Indra Topik Maulana, Rifa Safira, Inge Aprianti, Livia Syafnir, Reza Abdul Kodir
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Ahmad Dahlan 2021
Materias:	eucheuma spinosum streptococcus mutans shigella dysenteriae tasikmalaya tlc-bioautography Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/c51a04792d0a45e893a44fd01fd840a6
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spelling	oai:doaj.org-article:c51a04792d0a45e893a44fd01fd840a62021-11-18T01:04:37ZAntibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography10.12928/pharmaciana.v11i3.181442088-45592477-0256https://doaj.org/article/c51a04792d0a45e893a44fd01fd840a62021-11-01T00:00:00Zhttp://journal.uad.ac.id/index.php/PHARMACIANA/article/view/18144/pdf_213https://doaj.org/toc/2088-4559https://doaj.org/toc/2477-0256Streptococcus mutans (Gram-positive) and Shigella dysenteriae (Gram-negative) are two types of pathogen bacteria. The use of synthetic antibiotics against both bacteria is known to impact the bacteria's resistance. E. spinosum from Tasikmalaya is a potential macroalgae as a source of an antibacterial compound for both bacteria. The research aims to determine the antibacterial metabolite compound from E. spinosum originated from Tasikmalaya against S. mutans and S. dysenteriae. The research was conducted through several stages, starting from phytochemical screening, gradual maceration using hexane, ethyl acetate, and methanol, determination of antibacterial activity, and TLC-bioautography. Phytochemical screening showed that both raw material and extracts contained alkaloids, flavonoids, and steroids. The result showed that hexane, ethyl acetate, and methanol extract could inhibit the growth of S. dysenteriae starting from a concentration of 400 μg/mL. However, only ethyl acetate extract can inhibit the growth of S. mutans, starting from a concentration of 20 μg/mL. The chromatogram of the hexane extract showed the presence of 6 spots, ethyl acetate extract showed 5, and the methanol extract showed only 4, resulted from the elution system, respectively. The TLC-bioautography against S. dysenteriae showed that there was the presence of three clear zones on the ethyl acetate extract, detected as flavonoid, and three clear zones on the methanol extract. The TLC-bioautography against S. mutans showed one clear zone on the chromatogram of ethyl acetate extract. According to the AlCl3 spray reagent confirmation test, the active compound was the flavonoid group.Indra Topik MaulanaRifa SafiraInge ApriantiLivia SyafnirReza Abdul KodirUniversitas Ahmad Dahlanarticleeucheuma spinosumstreptococcus mutansshigella dysenteriaetasikmalayatlc-bioautographyPharmacy and materia medicaRS1-441ENPharmaciana, Vol 11, Iss 3, Pp 416-426 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eucheuma spinosum streptococcus mutans shigella dysenteriae tasikmalaya tlc-bioautography Pharmacy and materia medica RS1-441
spellingShingle	eucheuma spinosum streptococcus mutans shigella dysenteriae tasikmalaya tlc-bioautography Pharmacy and materia medica RS1-441 Indra Topik Maulana Rifa Safira Inge Aprianti Livia Syafnir Reza Abdul Kodir Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
description	Streptococcus mutans (Gram-positive) and Shigella dysenteriae (Gram-negative) are two types of pathogen bacteria. The use of synthetic antibiotics against both bacteria is known to impact the bacteria's resistance. E. spinosum from Tasikmalaya is a potential macroalgae as a source of an antibacterial compound for both bacteria. The research aims to determine the antibacterial metabolite compound from E. spinosum originated from Tasikmalaya against S. mutans and S. dysenteriae. The research was conducted through several stages, starting from phytochemical screening, gradual maceration using hexane, ethyl acetate, and methanol, determination of antibacterial activity, and TLC-bioautography. Phytochemical screening showed that both raw material and extracts contained alkaloids, flavonoids, and steroids. The result showed that hexane, ethyl acetate, and methanol extract could inhibit the growth of S. dysenteriae starting from a concentration of 400 μg/mL. However, only ethyl acetate extract can inhibit the growth of S. mutans, starting from a concentration of 20 μg/mL. The chromatogram of the hexane extract showed the presence of 6 spots, ethyl acetate extract showed 5, and the methanol extract showed only 4, resulted from the elution system, respectively. The TLC-bioautography against S. dysenteriae showed that there was the presence of three clear zones on the ethyl acetate extract, detected as flavonoid, and three clear zones on the methanol extract. The TLC-bioautography against S. mutans showed one clear zone on the chromatogram of ethyl acetate extract. According to the AlCl3 spray reagent confirmation test, the active compound was the flavonoid group.
format	article
author	Indra Topik Maulana Rifa Safira Inge Aprianti Livia Syafnir Reza Abdul Kodir
author_facet	Indra Topik Maulana Rifa Safira Inge Aprianti Livia Syafnir Reza Abdul Kodir
author_sort	Indra Topik Maulana
title	Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
title_short	Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
title_full	Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
title_fullStr	Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
title_full_unstemmed	Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography
title_sort	antibacterial compound from euchema spinosum originated from tasikmalaya west java against pathogen bacteria with tlc-bioautography
publisher	Universitas Ahmad Dahlan
publishDate	2021
url	https://doaj.org/article/c51a04792d0a45e893a44fd01fd840a6
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Antibacterial compound from Euchema spinosum originated from Tasikmalaya West Java against pathogen bacteria with TLC-bioautography

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