The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

Kengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed...

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Autores principales:	Xiaoyan Tao, Bo Liu, Quanwen Dou
Formato:	article
Lenguaje:	EN
Publicado:	Pensoft Publishers 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c51df204325649c0a838af833f31b627
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spelling	oai:doaj.org-article:c51df204325649c0a838af833f31b6272021-11-05T04:30:20ZThe Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes10.3897/compcytogen.v15.i4.715251993-078Xhttps://doaj.org/article/c51df204325649c0a838af833f31b6272021-11-01T00:00:00Zhttps://compcytogen.pensoft.net/article/71525/download/pdf/https://compcytogen.pensoft.net/article/71525/download/xml/https://compcytogen.pensoft.net/article/71525/https://doaj.org/toc/1993-078XKengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed using 14 single-gene probes covering all seven homoeologous groups, and four repetitive sequence probes 45S rDNA, 5S rDNA, pAs1, and (AAG)10 by FISH. Each chromosome of K. hirsuta was well characterized by homoeological determination and repeats distribution patterns. The synteny of chromosomes was strongly conserved in the St genome, whereas synteny of the Y and P genomes was more distorted. The collinearity of 1Y, 2Y, 3Y and 7Y might be interrupted in the Y genome. A new 5S rDNA site on 2Y might be translocated from 1Y. The short arm of 3Y might involve translocated segments from 7Y. The 7 Y was identified as involving a pericentric inversion. A reciprocal translocation between 2P and 4P, and tentative structural aberrations in the subtelomeric region of 1PL and 4PL, were observed in the P genome. Chromosome polymorphisms, which were mostly characterized by repeats amplification and deletion, varied between chromosomes, genomes, and different populations. However, two translocations involving a P genome segmental in 3YL and a non-Robertsonial reciprocal translocation between 4Y and 3P were identified in two independent populations. Moreover, the proportion of heterozygous karyotypes reached almost 35% in all materials, and almost 80% in the specific population. These results provide new insights into the genome organization of K. hirsuta and will facilitate genome dissection and germplasm utilization of this species.Xiaoyan TaoBo LiuQuanwen DouPensoft PublishersarticleGeneticsQH426-470ENComparative Cytogenetics, Vol 15, Iss 4, Pp 375-392 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Xiaoyan Tao Bo Liu Quanwen Dou The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
description	Kengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed using 14 single-gene probes covering all seven homoeologous groups, and four repetitive sequence probes 45S rDNA, 5S rDNA, pAs1, and (AAG)10 by FISH. Each chromosome of K. hirsuta was well characterized by homoeological determination and repeats distribution patterns. The synteny of chromosomes was strongly conserved in the St genome, whereas synteny of the Y and P genomes was more distorted. The collinearity of 1Y, 2Y, 3Y and 7Y might be interrupted in the Y genome. A new 5S rDNA site on 2Y might be translocated from 1Y. The short arm of 3Y might involve translocated segments from 7Y. The 7 Y was identified as involving a pericentric inversion. A reciprocal translocation between 2P and 4P, and tentative structural aberrations in the subtelomeric region of 1PL and 4PL, were observed in the P genome. Chromosome polymorphisms, which were mostly characterized by repeats amplification and deletion, varied between chromosomes, genomes, and different populations. However, two translocations involving a P genome segmental in 3YL and a non-Robertsonial reciprocal translocation between 4Y and 3P were identified in two independent populations. Moreover, the proportion of heterozygous karyotypes reached almost 35% in all materials, and almost 80% in the specific population. These results provide new insights into the genome organization of K. hirsuta and will facilitate genome dissection and germplasm utilization of this species.
format	article
author	Xiaoyan Tao Bo Liu Quanwen Dou
author_facet	Xiaoyan Tao Bo Liu Quanwen Dou
author_sort	Xiaoyan Tao
title	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
title_short	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
title_full	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
title_fullStr	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
title_full_unstemmed	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
title_sort	kengyilia hirsuta karyotype polymorphisms as revealed by fish with tandem repeats and single-gene probes
publisher	Pensoft Publishers
publishDate	2021
url	https://doaj.org/article/c51df204325649c0a838af833f31b627
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The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes

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