Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting

Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and qua...

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Autores principales:	Rod Chalk, William E. P. Greenland, Tiago Moreira, Jesse Coker, Shubhashish M. M. Mukhopadhyay, Eleanor Williams, Charlotte Manning, Tina Bohstedt, Rama McCrorie, Alejandra Fernandez-Cid, Nicola A. Burgess-Brown
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c526a34029cf4619809766a292866602
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spelling	oai:doaj.org-article:c526a34029cf4619809766a2928666022021-12-02T17:06:23ZIdentification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting10.1038/s42003-021-02455-w2399-3642https://doaj.org/article/c526a34029cf4619809766a2928666022021-08-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02455-whttps://doaj.org/toc/2399-3642Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.Rod ChalkWilliam E. P. GreenlandTiago MoreiraJesse CokerShubhashish M. M. MukhopadhyayEleanor WilliamsCharlotte ManningTina BohstedtRama McCrorieAlejandra Fernandez-CidNicola A. Burgess-BrownNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
description	Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.
format	article
author	Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown
author_facet	Rod Chalk William E. P. Greenland Tiago Moreira Jesse Coker Shubhashish M. M. Mukhopadhyay Eleanor Williams Charlotte Manning Tina Bohstedt Rama McCrorie Alejandra Fernandez-Cid Nicola A. Burgess-Brown
author_sort	Rod Chalk
title	Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
title_short	Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
title_full	Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
title_fullStr	Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
title_full_unstemmed	Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting
title_sort	identification, mapping and relative quantitation of sars-cov-2 spike glycopeptides by mass-retention time fingerprinting
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c526a34029cf4619809766a292866602
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Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting

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