Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.

CD8+ T cells can be primed by peptides derived from endogenous proteins (direct presentation), or exogenously acquired protein (cross-presentation). However, the relative ability of these two pathways to prime CD8+ T cells during a viral infection remains controversial. Cytomegaloviruses (CMVs) can...

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Autores principales:	Christopher M Snyder, Jane E Allan, Elizabeth L Bonnett, Carmen M Doom, Ann B Hill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c52bbda24960495c92f506ba0d98c985
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spelling	oai:doaj.org-article:c52bbda24960495c92f506ba0d98c9852021-11-25T06:25:28ZCross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.1932-620310.1371/journal.pone.0009681https://doaj.org/article/c52bbda24960495c92f506ba0d98c9852010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20300633/?tool=EBIhttps://doaj.org/toc/1932-6203CD8+ T cells can be primed by peptides derived from endogenous proteins (direct presentation), or exogenously acquired protein (cross-presentation). However, the relative ability of these two pathways to prime CD8+ T cells during a viral infection remains controversial. Cytomegaloviruses (CMVs) can infect professional antigen presenting cells (APCs), including dendritic cells, thus providing peptides for direct presentation. However, the viral immune evasion genes profoundly impair recognition of infected cells by CD8+ T cells. Nevertheless, CMV infection elicits a very strong CD8+ T cell response, prompting its recent use as a vaccine vector. We have shown previously that deleting the immune evasion genes from murine cytomegalovirus (MCMV) that target class I MHC presentation, has no impact on the size or breadth of the CD8+ T cell response elicited by infection, suggesting that the majority of MCMV-specific CD8+ T cells in vivo are not directly primed by infected professional APCs. Here we use a novel spread-defective mutant of MCMV, lacking the essential glycoprotein gL, to show that cross-presentation alone can account for the majority of MCMV-specific CD8+ T cell responses to the virus. Our data support the conclusion that cross-presentation is the primary mode of antigen presentation by which CD8+ T cells are primed during MCMV infection.Christopher M SnyderJane E AllanElizabeth L BonnettCarmen M DoomAnn B HillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 3, p e9681 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher M Snyder Jane E Allan Elizabeth L Bonnett Carmen M Doom Ann B Hill Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
description	CD8+ T cells can be primed by peptides derived from endogenous proteins (direct presentation), or exogenously acquired protein (cross-presentation). However, the relative ability of these two pathways to prime CD8+ T cells during a viral infection remains controversial. Cytomegaloviruses (CMVs) can infect professional antigen presenting cells (APCs), including dendritic cells, thus providing peptides for direct presentation. However, the viral immune evasion genes profoundly impair recognition of infected cells by CD8+ T cells. Nevertheless, CMV infection elicits a very strong CD8+ T cell response, prompting its recent use as a vaccine vector. We have shown previously that deleting the immune evasion genes from murine cytomegalovirus (MCMV) that target class I MHC presentation, has no impact on the size or breadth of the CD8+ T cell response elicited by infection, suggesting that the majority of MCMV-specific CD8+ T cells in vivo are not directly primed by infected professional APCs. Here we use a novel spread-defective mutant of MCMV, lacking the essential glycoprotein gL, to show that cross-presentation alone can account for the majority of MCMV-specific CD8+ T cell responses to the virus. Our data support the conclusion that cross-presentation is the primary mode of antigen presentation by which CD8+ T cells are primed during MCMV infection.
format	article
author	Christopher M Snyder Jane E Allan Elizabeth L Bonnett Carmen M Doom Ann B Hill
author_facet	Christopher M Snyder Jane E Allan Elizabeth L Bonnett Carmen M Doom Ann B Hill
author_sort	Christopher M Snyder
title	Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
title_short	Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
title_full	Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
title_fullStr	Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
title_full_unstemmed	Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.
title_sort	cross-presentation of a spread-defective mcmv is sufficient to prime the majority of virus-specific cd8+ t cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/c52bbda24960495c92f506ba0d98c985
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Cross-presentation of a spread-defective MCMV is sufficient to prime the majority of virus-specific CD8+ T cells.

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