Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice

Arsenic can cause neurodegenerative diseases of the brain, but the definite mechanism is still unknown. In this study, to discuss the disturbances on brain metabolome and lipidome under subchronic arsenic exposure, we treated mice with the arsenic-containing feed (concentration of total arsenic = 30...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chenfei Wang, Hongyu Deng, Dongbin Wang, Jiating Wang, Hairong Huang, Jiayi Qiu, Yinfei Li, Tangbin Zou, Lianxian Guo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Metabolomics Lipidomics Brain Gut microbiome Arsenic Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/c52c2875e5934f81a15297a0d14fc821
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c52c2875e5934f81a15297a0d14fc821
record_format	dspace
spelling	oai:doaj.org-article:c52c2875e5934f81a15297a0d14fc8212021-11-20T04:55:36ZChanges in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice0147-651310.1016/j.ecoenv.2021.112935https://doaj.org/article/c52c2875e5934f81a15297a0d14fc8212021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010472https://doaj.org/toc/0147-6513Arsenic can cause neurodegenerative diseases of the brain, but the definite mechanism is still unknown. In this study, to discuss the disturbances on brain metabolome and lipidome under subchronic arsenic exposure, we treated mice with the arsenic-containing feed (concentration of total arsenic = 30 mg/kg) prepared in accordance with the proportion of rice arsenicals for 16 weeks and performed metabolomics and lipidomics studies respectively using UHPLC-Triple-TOF-MS/MS and UHPLC-Q Exactive Focus MS/MS on mice brain. In addition, the distributions of arsenical metabolites along the feed-gut-blood-brain chain were analyzed by ICP-MS and HPLC-ICP-MS, and fecal microbial variations were investigated by 16 s sequencing. The data showed that although only a tiny amount of arsenic (DMA=0.101 mg/kg, uAs=0.071 mg/kg) enters the brain through the blood-brain barrier, there were significant changes in brain metabolism, including 118 metabolites and 17 lipids. These different metabolites were involved in 30 distinct pathways, including glycometabolism, and metabolisms of lipid, nucleic acid, and amino acid were previously reported to be correlated with neurodegenerative diseases. Additionally, these different metabolites were significantly correlated with 12 gut bacterial OTUs, among which Lachnospiraceae, Muribaculaceae, Ruminococcaceae, and Erysipelotrichaceae were also previously reported to be related to the distortion of metabolism, indicating that the disturbance of metabolism in the brain may be associated with the disturbance of gut microbes induced by arsenic. Thus, the current study demonstrated that the brain metabolome and lipidome were significantly disturbed under subchronic arsenic exposure, and the disturbances also significantly correlated with some gut microbiome and may be associated with neurodegenerative diseases. Although preliminary, the results shed some light on the pathophysiology of arsenic-caused neurodegenerative diseases.Chenfei WangHongyu DengDongbin WangJiating WangHairong HuangJiayi QiuYinfei LiTangbin ZouLianxian GuoElsevierarticleMetabolomicsLipidomicsBrainGut microbiomeArsenicEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112935- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Metabolomics Lipidomics Brain Gut microbiome Arsenic Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Metabolomics Lipidomics Brain Gut microbiome Arsenic Environmental pollution TD172-193.5 Environmental sciences GE1-350 Chenfei Wang Hongyu Deng Dongbin Wang Jiating Wang Hairong Huang Jiayi Qiu Yinfei Li Tangbin Zou Lianxian Guo Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
description	Arsenic can cause neurodegenerative diseases of the brain, but the definite mechanism is still unknown. In this study, to discuss the disturbances on brain metabolome and lipidome under subchronic arsenic exposure, we treated mice with the arsenic-containing feed (concentration of total arsenic = 30 mg/kg) prepared in accordance with the proportion of rice arsenicals for 16 weeks and performed metabolomics and lipidomics studies respectively using UHPLC-Triple-TOF-MS/MS and UHPLC-Q Exactive Focus MS/MS on mice brain. In addition, the distributions of arsenical metabolites along the feed-gut-blood-brain chain were analyzed by ICP-MS and HPLC-ICP-MS, and fecal microbial variations were investigated by 16 s sequencing. The data showed that although only a tiny amount of arsenic (DMA=0.101 mg/kg, uAs=0.071 mg/kg) enters the brain through the blood-brain barrier, there were significant changes in brain metabolism, including 118 metabolites and 17 lipids. These different metabolites were involved in 30 distinct pathways, including glycometabolism, and metabolisms of lipid, nucleic acid, and amino acid were previously reported to be correlated with neurodegenerative diseases. Additionally, these different metabolites were significantly correlated with 12 gut bacterial OTUs, among which Lachnospiraceae, Muribaculaceae, Ruminococcaceae, and Erysipelotrichaceae were also previously reported to be related to the distortion of metabolism, indicating that the disturbance of metabolism in the brain may be associated with the disturbance of gut microbes induced by arsenic. Thus, the current study demonstrated that the brain metabolome and lipidome were significantly disturbed under subchronic arsenic exposure, and the disturbances also significantly correlated with some gut microbiome and may be associated with neurodegenerative diseases. Although preliminary, the results shed some light on the pathophysiology of arsenic-caused neurodegenerative diseases.
format	article
author	Chenfei Wang Hongyu Deng Dongbin Wang Jiating Wang Hairong Huang Jiayi Qiu Yinfei Li Tangbin Zou Lianxian Guo
author_facet	Chenfei Wang Hongyu Deng Dongbin Wang Jiating Wang Hairong Huang Jiayi Qiu Yinfei Li Tangbin Zou Lianxian Guo
author_sort	Chenfei Wang
title	Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
title_short	Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
title_full	Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
title_fullStr	Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
title_full_unstemmed	Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
title_sort	changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c52c2875e5934f81a15297a0d14fc821
work_keys_str_mv	AT chenfeiwang changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT hongyudeng changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT dongbinwang changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT jiatingwang changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT haironghuang changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT jiayiqiu changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT yinfeili changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT tangbinzou changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice AT lianxianguo changesinmetabolomicsandlipidomicsinbraintissueandtheircorrelationswiththegutmicrobiomeafterchronicfoodderivedarsenicexposureinmice
_version_	1718419738621640704

Changes in metabolomics and lipidomics in brain tissue and their correlations with the gut microbiome after chronic food-derived arsenic exposure in mice

Ejemplares similares