MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus

Abstract Embryos of the annual killifish Austrofundulus limnaeus are the most anoxia-tolerant vertebrate. Annual killifish inhabit ephemeral ponds, producing drought and anoxia-tolerant embryos, which allows the species to persist generation after generation. Anoxia tolerance and physiology vary by...

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Autores principales:	Claire L. Riggs, Steven Cody Woll, Jason E. Podrabsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c52e2f2c4f7c47cfa565a45c2cc1c45a
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spelling	oai:doaj.org-article:c52e2f2c4f7c47cfa565a45c2cc1c45a2021-12-02T13:56:50ZMitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus10.1038/s41598-019-56231-22045-2322https://doaj.org/article/c52e2f2c4f7c47cfa565a45c2cc1c45a2019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56231-2https://doaj.org/toc/2045-2322Abstract Embryos of the annual killifish Austrofundulus limnaeus are the most anoxia-tolerant vertebrate. Annual killifish inhabit ephemeral ponds, producing drought and anoxia-tolerant embryos, which allows the species to persist generation after generation. Anoxia tolerance and physiology vary by developmental stage, creating a unique opportunity for comparative study within the species. A recent study of small ncRNA expression in A. limnaeus embryos in response to anoxia and aerobic recovery revealed small ncRNAs with expression patterns that suggest a role in supporting anoxia tolerance. MitosRNAs, small ncRNAs derived from the mitochondrial genome, emerged as an interesting group of these sequences. MitosRNAs derived from mitochondrial tRNAs were differentially expressed in developing embryos and isolated cells exhibiting extreme anoxia tolerance. In this study we focus on expression of mitosRNAs derived from tRNA-cysteine, and their subcellular and organismal localization in order to consider possible function. These tRNA-cys mitosRNAs appear enriched in the mitochondria, particularly near the nucleus, and also appear to be present in the cytoplasm. We provide evidence that mitosRNAs are generated in the mitochondria in response to anoxia, though the precise mechanism of biosynthesis remains unclear. MitosRNAs derived from tRNA-cys localize to numerous tissues, and increase in the anterior brain during anoxia. We hypothesize that these RNAs may play a role in regulating gene expression that supports extreme anoxia tolerance.Claire L. RiggsSteven Cody WollJason E. PodrabskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-17 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire L. Riggs Steven Cody Woll Jason E. Podrabsky MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
description	Abstract Embryos of the annual killifish Austrofundulus limnaeus are the most anoxia-tolerant vertebrate. Annual killifish inhabit ephemeral ponds, producing drought and anoxia-tolerant embryos, which allows the species to persist generation after generation. Anoxia tolerance and physiology vary by developmental stage, creating a unique opportunity for comparative study within the species. A recent study of small ncRNA expression in A. limnaeus embryos in response to anoxia and aerobic recovery revealed small ncRNAs with expression patterns that suggest a role in supporting anoxia tolerance. MitosRNAs, small ncRNAs derived from the mitochondrial genome, emerged as an interesting group of these sequences. MitosRNAs derived from mitochondrial tRNAs were differentially expressed in developing embryos and isolated cells exhibiting extreme anoxia tolerance. In this study we focus on expression of mitosRNAs derived from tRNA-cysteine, and their subcellular and organismal localization in order to consider possible function. These tRNA-cys mitosRNAs appear enriched in the mitochondria, particularly near the nucleus, and also appear to be present in the cytoplasm. We provide evidence that mitosRNAs are generated in the mitochondria in response to anoxia, though the precise mechanism of biosynthesis remains unclear. MitosRNAs derived from tRNA-cys localize to numerous tissues, and increase in the anterior brain during anoxia. We hypothesize that these RNAs may play a role in regulating gene expression that supports extreme anoxia tolerance.
format	article
author	Claire L. Riggs Steven Cody Woll Jason E. Podrabsky
author_facet	Claire L. Riggs Steven Cody Woll Jason E. Podrabsky
author_sort	Claire L. Riggs
title	MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
title_short	MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
title_full	MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
title_fullStr	MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
title_full_unstemmed	MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus
title_sort	mitosrnas and extreme anoxia tolerance in embryos of the annual killifish austrofundulus limnaeus
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c52e2f2c4f7c47cfa565a45c2cc1c45a
work_keys_str_mv	AT clairelriggs mitosrnasandextremeanoxiatoleranceinembryosoftheannualkillifishaustrofunduluslimnaeus AT stevencodywoll mitosrnasandextremeanoxiatoleranceinembryosoftheannualkillifishaustrofunduluslimnaeus AT jasonepodrabsky mitosrnasandextremeanoxiatoleranceinembryosoftheannualkillifishaustrofunduluslimnaeus
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MitosRNAs and extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus

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