Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>

Single-stranded DNA aptamers as affinity molecules for the rapid, reliable detection of intestinal bacteria are of particular interest to equip health systems with novel robust and cheap diagnostic tools for monitoring the success of supplementation strategies with selected probiotic gut bacteria in...

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Autores principales:	Hu Xing, Ann-Kathrin Kissmann, Heinz Fabian Raber, Markus Krämer, Valerie Amann, Kathrin Kohn, Tanja Weil, Frank Rosenau
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aptamer <i>Parabacteroides distasonis</i> FluCell-SELEX biosensor development Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c52f967533f74a57b469bf0f6fef56d1
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spelling	oai:doaj.org-article:c52f967533f74a57b469bf0f6fef56d12021-11-25T18:24:51ZPolyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>10.3390/microorganisms91122842076-2607https://doaj.org/article/c52f967533f74a57b469bf0f6fef56d12021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2284https://doaj.org/toc/2076-2607Single-stranded DNA aptamers as affinity molecules for the rapid, reliable detection of intestinal bacteria are of particular interest to equip health systems with novel robust and cheap diagnostic tools for monitoring the success of supplementation strategies with selected probiotic gut bacteria in the fight against major widespread threats, such as obesity and neurodegenerative diseases. The human gut bacterium <i>Parabacteroides distasonis</i> (<i>P. distasonis</i>) is positively associated with diseases such as obesity, non-alcoholic fatty liver disease and multiple sclerosis with reduced cell counts in these diseases and is thus a promising potential probiotic bacterium for future microbial supplementation. In this paper we report on the evolution of a specific polyclonal aptamer library by the fluorescence based FluCell-SELEX directed against whole cells of <i>P. distasonis</i> that specifically and efficiently binds and labels <i>P. distasonis</i>. The aptamer library showed high binding affinity and was suited to quantitatively discriminate <i>P. distasonis</i> from other prominent gut bacteria also in mixtures. We believe that this library against a promising probiotic bacterium as a prototype may open new routes towards the development of novel biosensors for the easy and efficient quantitative monitoring of microbial abundance in human microbiomes in general.Hu XingAnn-Kathrin KissmannHeinz Fabian RaberMarkus KrämerValerie AmannKathrin KohnTanja WeilFrank RosenauMDPI AGarticleaptamer<i>Parabacteroides distasonis</i>FluCell-SELEXbiosensor developmentBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2284, p 2284 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aptamer <i>Parabacteroides distasonis</i> FluCell-SELEX biosensor development Biology (General) QH301-705.5
spellingShingle	aptamer <i>Parabacteroides distasonis</i> FluCell-SELEX biosensor development Biology (General) QH301-705.5 Hu Xing Ann-Kathrin Kissmann Heinz Fabian Raber Markus Krämer Valerie Amann Kathrin Kohn Tanja Weil Frank Rosenau Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
description	Single-stranded DNA aptamers as affinity molecules for the rapid, reliable detection of intestinal bacteria are of particular interest to equip health systems with novel robust and cheap diagnostic tools for monitoring the success of supplementation strategies with selected probiotic gut bacteria in the fight against major widespread threats, such as obesity and neurodegenerative diseases. The human gut bacterium <i>Parabacteroides distasonis</i> (<i>P. distasonis</i>) is positively associated with diseases such as obesity, non-alcoholic fatty liver disease and multiple sclerosis with reduced cell counts in these diseases and is thus a promising potential probiotic bacterium for future microbial supplementation. In this paper we report on the evolution of a specific polyclonal aptamer library by the fluorescence based FluCell-SELEX directed against whole cells of <i>P. distasonis</i> that specifically and efficiently binds and labels <i>P. distasonis</i>. The aptamer library showed high binding affinity and was suited to quantitatively discriminate <i>P. distasonis</i> from other prominent gut bacteria also in mixtures. We believe that this library against a promising probiotic bacterium as a prototype may open new routes towards the development of novel biosensors for the easy and efficient quantitative monitoring of microbial abundance in human microbiomes in general.
format	article
author	Hu Xing Ann-Kathrin Kissmann Heinz Fabian Raber Markus Krämer Valerie Amann Kathrin Kohn Tanja Weil Frank Rosenau
author_facet	Hu Xing Ann-Kathrin Kissmann Heinz Fabian Raber Markus Krämer Valerie Amann Kathrin Kohn Tanja Weil Frank Rosenau
author_sort	Hu Xing
title	Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
title_short	Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
title_full	Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
title_fullStr	Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
title_full_unstemmed	Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>
title_sort	polyclonal aptamers for specific fluorescence labeling and quantification of the health relevant human gut bacterium <i>parabacteroides distasonis</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c52f967533f74a57b469bf0f6fef56d1
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Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium <i>Parabacteroides distasonis</i>

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