A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area
Abstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c532b10f3103458694357cf986f8996e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c532b10f3103458694357cf986f8996e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c532b10f3103458694357cf986f8996e2021-12-02T15:08:22ZA comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area10.1038/s41598-018-33937-32045-2322https://doaj.org/article/c532b10f3103458694357cf986f8996e2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33937-3https://doaj.org/toc/2045-2322Abstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships.Timothy P. JenkinsFabio FormentiCecilia CastroChiara PiubelliFrancesca PerandinDora BuonfrateDomenico OtrantoJulian L. GriffinLutz KrauseZeno BisoffiCinzia CantacessiNature PortfolioarticleStrongyloides StercoralisStercoralis InfectionMicrobial Alpha DiversityDecreased Beta DiversityFecal MetabolomeMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Strongyloides Stercoralis Stercoralis Infection Microbial Alpha Diversity Decreased Beta Diversity Fecal Metabolome Medicine R Science Q |
| spellingShingle |
Strongyloides Stercoralis Stercoralis Infection Microbial Alpha Diversity Decreased Beta Diversity Fecal Metabolome Medicine R Science Q Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| description |
Abstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships. |
| format |
article |
| author |
Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi |
| author_facet |
Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi |
| author_sort |
Timothy P. Jenkins |
| title |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| title_short |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| title_full |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| title_fullStr |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| title_full_unstemmed |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
| title_sort |
comprehensive analysis of the faecal microbiome and metabolome of strongyloides stercoralis infected volunteers from a non-endemic area |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/c532b10f3103458694357cf986f8996e |
| work_keys_str_mv |
AT timothypjenkins acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT fabioformenti acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT ceciliacastro acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT chiarapiubelli acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT francescaperandin acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT dorabuonfrate acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT domenicootranto acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT julianlgriffin acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT lutzkrause acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT zenobisoffi acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT cinziacantacessi acomprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT timothypjenkins comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT fabioformenti comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT ceciliacastro comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT chiarapiubelli comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT francescaperandin comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT dorabuonfrate comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT domenicootranto comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT julianlgriffin comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT lutzkrause comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT zenobisoffi comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea AT cinziacantacessi comprehensiveanalysisofthefaecalmicrobiomeandmetabolomeofstrongyloidesstercoralisinfectedvolunteersfromanonendemicarea |
| _version_ |
1718388212790984704 |